FastQCFastQC Report
Thu 26 May 2016
SRR2094056_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094056_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13117
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA350.2668293054814363RNA PCR Primer, Index 31 (96% over 29bp)
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTA220.16772127773118853No Hit
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAA200.152473888846535RNA PCR Primer, Index 31 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC190.1448501944042083No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.12960280551955478No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC150.11435541663490127TruSeq Adapter, Index 3 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.11435541663490127No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCG300.004965814329.9999987
ATACACA300.004965814329.9999981
Produced by FastQC (version 0.11.5)