FastQCFastQC Report
Thu 26 May 2016
SRR2094055_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094055_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2793649
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56340.20167172039150227No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC54650.19562228468930778RNA PCR Primer, Index 33 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC53000.18971603089722439No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52030.1862438695770299No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43770.15667680513908513No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA33300.11919894016750136RNA PCR Primer, Index 33 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG29910.10706427328558456RNA PCR Primer, Index 33 (96% over 25bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23400.030.6976551
GTATCAA50450.023.1652281
CATTCCG2900.018.6192689
TAGTCGG1902.3646862E-1117.76402740
CCCCGTA3350.017.46354125
GCGTCGT3900.017.30853827
GGTCGGT2105.456968E-1217.14154811
CCGTATT3550.017.11351427
CCCGTAT3550.017.11351426
ATCAACG83150.017.073262
TATCAAC83450.016.9728471
CGTATTA3450.016.95735228
TCAACGC84000.016.9272793
CAACGCA85650.016.5749154
AACGCAG87600.016.3086875
TTAGTCG2101.07320375E-1016.07135239
CGTCGTG4250.015.8831328
CGAAATC3850.015.77801513
GTCGGTT2009.313226E-1015.74879812
CGGTTTG2453.6379788E-1215.61105314
Produced by FastQC (version 0.11.5)