FastQCFastQC Report
Thu 26 May 2016
SRR2094054_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094054_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5367858
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84380.1571949183454555No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC73150.13627409666947227No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68210.1270711706606248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52450.030.4643271
GTATCAA102700.021.4834021
CGCTATC22000.018.10158733
CGCTACG21850.017.9169428
CTCGTAT21650.017.77118739
GACGCTA22250.017.49437126
ACGCTAC22400.017.47766927
TACGCTA23200.017.45623431
TATCTCG22600.017.4220736
CGAGACG22900.017.39033123
TATGCCG22350.017.21507643
ACGCTAT23200.017.16529732
TCTCGTA22350.017.11392438
GCTACGC23750.016.86283329
ATGCCGT23600.016.59020844
ACGAGAC24500.016.5301422
TGCCGTC23900.016.4759645
TCGTATG23500.016.46792240
CTACGCT24700.016.39644630
ATCTCCG24350.016.35400610
Produced by FastQC (version 0.11.5)