FastQCFastQC Report
Thu 26 May 2016
SRR2094053_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094053_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645053
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC49310.299747181397803RNA PCR Primer, Index 39 (95% over 24bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA38010.2310563854173695RNA PCR Primer, Index 39 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC30400.18479647768187407RNA PCR Primer, Index 39 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG28650.17415852255216094RNA PCR Primer, Index 39 (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25040.1522139408274384No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20430.12419052760002261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12800.028.3250581
GTATCAA26200.021.9176431
CGTCGTA550.00393849620.45342610
TATCAAC47600.019.6807371
ATCAACG48500.019.3906922
TGCTTGA11650.019.31635745
TCAACGC50050.018.7002753
GAATGCG1102.2583627E-618.40808511
CAACGCA51800.018.1553784
AACGCAG53100.017.6261545
CTGCTTG14100.017.55596744
GTACCGT1156.788519E-515.6513176
TCGCGTC1156.788519E-515.65131717
ACAGTGC6250.014.7591928
TAGGACC2456.91216E-1114.6930734
ACGCAGA65150.014.4351276
TATACCG950.005379269414.2097495
TCTGCTT17400.014.09619943
CGCAGAG66400.014.0278477
ACTGCGC1453.107558E-513.9647538
Produced by FastQC (version 0.11.5)