FastQCFastQC Report
Thu 26 May 2016
SRR2094052_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094052_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5083214
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC99030.19481768817917167RNA PCR Primer, Index 27 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC69370.13646877743097183RNA PCR Primer, Index 27 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69070.13587859964188012No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51050.1004285871104384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42300.028.1711731
GTATCAA82200.021.3479061
ATCAACG112250.015.1320863
TATCAAC113750.015.0512112
TCAACGC114600.014.8021554
CAACGCA117100.014.4477145
ACGCCCG2052.185152E-814.26725821
TATACCG1605.0396247E-614.0610645
AACGCAG120750.014.0296226
ATCTCCG36500.013.68353410
GTACCGT2959.640644E-1112.9647786
CCGTCTT29000.012.493299544
TATCTCG40150.012.27352836
CGTGCGC4800.012.18625510
CGAGACG41050.012.16732323
CTCGTAT40500.012.11094739
TCTCGTA40650.012.06685138
ACGGACT40100.012.06330927
ACGCAGA141200.011.9817797
CGCAGAG141800.011.9786798
Produced by FastQC (version 0.11.5)