FastQCFastQC Report
Thu 26 May 2016
SRR2094051_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094051_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726909
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC55290.7606179040292527RNA PCR Primer, Index 26 (100% over 23bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA38540.5301901613544474RNA PCR Primer, Index 26 (100% over 29bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG33610.4623687421671764RNA PCR Primer, Index 26 (100% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC22940.3155828308632855No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTT16000.22011008255503786RNA PCR Primer, Index 26 (100% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15720.21625815611032465No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAA10720.14747375531187534RNA PCR Primer, Index 26 (100% over 29bp)
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC9360.12876439829469713RNA PCR Primer, Index 26 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9190.12642572866754986No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGC250.002106852635.9991238
GGTATCA4350.028.4593261
TGCTTGA7200.026.56367745
TATCAAC22200.023.928211
CTGCTTG8650.022.6310644
TCGCGTC602.4667065E-422.4994517
ATCAACG24000.022.4057032
GTATCAA10300.022.2902071
TCAACGC23950.022.1706493
CAACGCA24350.021.8988484
AACGCAG25900.020.9357815
CTTATAC8500.020.9198861
CGCGTCC654.236865E-420.76872418
TTATACA9850.019.1873472
TGTACCG600.006509165718.7495425
TCTGCTT11200.017.47845343
TATACAC12250.017.4485533
CTTCTGC11700.016.92266341
CGACCAT800.001710308316.87458810
CGTCCAT800.001710308316.87458820
Produced by FastQC (version 0.11.5)