FastQCFastQC Report
Thu 26 May 2016
SRR2094050_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094050_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5164060
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87000.16847209366273824No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC79690.15431656487337483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68360.1323764634802849No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC52020.10073469324523728RNA PCR Primer, Index 17 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51950.10059914098596841No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52150.030.9351
GTATCAA101500.022.1541921
TATCAAC137100.016.2623672
ATCAACG138000.016.0747933
TCAACGC139150.015.9419444
CAACGCA143550.015.4846475
AACGCAG147400.015.1412526
AATACCG1654.2118882E-714.9987935
ATCTCCG28400.014.89316810
TCTCGTA31050.013.55120938
CTCGTAT30700.013.41162239
ATGCCGT30900.013.25290144
TATGCCG31150.013.21851443
ACGCAGA169400.013.0818877
TGCCGTC31350.013.06355345
TCGTATG32250.013.04634640
AATCTCG31850.012.99914736
ACGAGAC32900.012.99312622
GAGTACT109050.012.97694812
CGCAGAG171950.012.87488
Produced by FastQC (version 0.11.5)