FastQCFastQC Report
Thu 26 May 2016
SRR2094049_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094049_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5471558
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC135050.24682183758264098No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC107910.19721987777521502RNA PCR Primer, Index 33 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95230.1740454912476483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79070.14451094185604904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71560.1307854179741858No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA65480.11967340929219794RNA PCR Primer, Index 33 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG63020.11517743209520945RNA PCR Primer, Index 33 (96% over 25bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61850.028.555541
GTATCAA119700.021.6053751
GACCGTA850.002568095515.8811687
TGCTTGA23750.015.444245
TATCAAC172050.014.98580552
ATCAACG172250.014.8769753
TCAACGC174250.014.6803994
CAACGCA177050.014.4736455
AACGCAG179150.014.34166056
CGTATAG1200.001658724913.12402110
CGTCGTA2602.6739144E-912.97980210
ATCTCCG53300.012.95869610
CGTGCGC4350.012.9300710
TTCGCTA1403.6117536E-412.85653835
ATACCGT4250.012.7049366
AAGGCGT6500.012.4606096
ACGCAGA207150.012.3053827
CCGTCTT43750.012.29309744
CGGCTAG3304.9112714E-1112.27327127
GTACATG163050.012.2136531
Produced by FastQC (version 0.11.5)