FastQCFastQC Report
Thu 26 May 2016
SRR2094048_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094048_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2224460
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC54510.24504823642591908TruSeq Adapter, Index 10 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC45140.20292565386655637No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA39360.17694181958767521TruSeq Adapter, Index 10 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32800.14745151632306266No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG31680.1424165864973971RNA PCR Primer, Index 10 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30000.1348641917588988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18550.028.407711
GTATCAA36150.021.1804371
CGTATTA750.001110652218.00003628
TGCTTGA12250.017.45215445
TATCAAC61750.017.3229141
TCAACGC62200.017.1813533
ATCAACG62200.017.1451822
TACGCTG1205.180711E-616.8738965
CAACGCA64250.016.5281014
AACGCAG65700.016.2318135
ATAAGAC6000.015.748973
TATACTG8200.015.3648485
CGTCTTC21850.014.31610445
CCGTCTT21950.014.14835744
ATCTCCG22550.014.0678157
GCCGTCT22800.013.81737143
TCGTTAC1656.914597E-613.63669823
GGGCCGA1656.9210237E-613.6354726
ACGCAGA79950.013.3387166
CGCAGAG80600.013.2311447
Produced by FastQC (version 0.11.5)