FastQCFastQC Report
Wed 25 May 2016
SRR2088722_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088722_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1707743
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT274561.6077360586458267No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT261231.5296798171621842No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT211081.2360173632683606No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109190.6393819210501814No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG79600.46611228972977786No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG70710.4140552764672436No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA68470.40093854871605394No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG60570.35467866066498294No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA59590.34894009227383743No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA49350.2889779082683987No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA35220.20623712115933135No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC34310.2009084505104105No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA32600.19089523423606478No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA31900.186796256813818No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29880.1749677791096201No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG27630.1617924945381126No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA27150.15898176716285764No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26650.1560539261469671No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25190.14750463038056663No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG24800.145220914388172No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG23800.13936523235639084No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC21970.1286493342382314No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA21900.12823943649600672No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA20180.11816766340134319No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC19790.11588394740894854No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT18380.10762743574413715No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT17880.10469959472824658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81950.024.2452741
GTATTGG5450.021.3853231
ATTGGAC7400.017.53
CGAATTA752.0680073E-417.26666615
GGACGCT650.001580359217.07692331
GTTATGC803.3837697E-416.18753
CATTGCG700.002593184615.85714329
CAATGCG1059.348334E-615.85714219
GTATCAA130150.015.2804462
TAGGACG855.366045E-415.2352944
AATGCGA1353.9751285E-715.07407520
TATTGGA7400.015.02
GGACCCT9450.014.8783076
TTGGACC10250.014.7999994
CTTACTC1405.9992453E-714.5357153
TCTACAC2059.276846E-1114.4390253
GACCCTC9250.014.47
GTATTGA1803.3323886E-914.3888881
TGCGACG908.276916E-414.38888822
TCCGGTC1702.4385372E-814.1470598
Produced by FastQC (version 0.11.5)