##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088722_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1707743
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42386237273407	31.0	31.0	34.0	30.0	34.0
2	31.62421453345146	31.0	31.0	34.0	30.0	34.0
3	31.679475775921787	31.0	31.0	34.0	30.0	34.0
4	35.4161849880222	37.0	35.0	37.0	33.0	37.0
5	35.31919029971137	37.0	35.0	37.0	33.0	37.0
6	35.386491410007245	37.0	35.0	37.0	33.0	37.0
7	35.22275131562536	37.0	35.0	37.0	32.0	37.0
8	35.285465084617535	37.0	35.0	37.0	32.0	37.0
9	36.83682029438856	39.0	37.0	39.0	32.0	39.0
10	36.68524362272309	39.0	35.0	39.0	32.0	39.0
11	36.76797972528653	39.0	37.0	39.0	32.0	39.0
12	36.61056259636257	39.0	35.0	39.0	32.0	39.0
13	36.64818652455317	39.0	35.0	39.0	32.0	39.0
14	37.52543561882555	39.0	36.0	41.0	32.0	41.0
15	37.54664138573544	39.0	36.0	41.0	32.0	41.0
16	37.41616800654431	39.0	36.0	41.0	32.0	41.0
17	37.526929403311854	39.0	36.0	41.0	32.0	41.0
18	37.508662603213715	39.0	36.0	41.0	32.0	41.0
19	37.58037128537491	39.0	37.0	41.0	32.0	41.0
20	37.468956394492615	39.0	36.0	41.0	32.0	41.0
21	37.4049994642051	39.0	36.0	41.0	32.0	41.0
22	37.283060741575284	39.0	36.0	41.0	31.0	41.0
23	37.07583576685719	39.0	36.0	41.0	31.0	41.0
24	36.91659810638954	39.0	36.0	40.0	31.0	41.0
25	37.07499957546305	39.0	36.0	41.0	31.0	41.0
26	36.9214905287271	39.0	36.0	41.0	31.0	41.0
27	36.79844449662508	39.0	35.0	40.0	30.0	41.0
28	36.612347993814055	39.0	35.0	40.0	30.0	41.0
29	36.485848866017896	39.0	35.0	40.0	30.0	41.0
30	36.23597637349414	38.0	35.0	40.0	30.0	41.0
31	36.016605543105726	38.0	35.0	40.0	30.0	41.0
32	35.82127989984441	38.0	35.0	40.0	29.0	41.0
33	35.650878381583176	38.0	34.0	40.0	28.0	41.0
34	35.44563496966464	38.0	34.0	40.0	27.0	41.0
35	35.270244995880525	38.0	34.0	40.0	26.0	41.0
36	35.04864490734261	38.0	34.0	40.0	25.0	41.0
37	34.90829943381411	38.0	33.0	40.0	24.0	41.0
38	34.68244343557549	38.0	33.0	40.0	24.0	41.0
39	34.54491103169505	38.0	33.0	40.0	23.0	41.0
40	34.316234936989936	38.0	33.0	40.0	22.0	41.0
41	34.13163221866522	38.0	33.0	40.0	21.0	41.0
42	33.79226850878616	38.0	33.0	40.0	18.0	41.0
43	33.40720061508084	37.0	32.0	40.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	4.0
14	3.0
15	14.0
16	20.0
17	48.0
18	129.0
19	324.0
20	739.0
21	1447.0
22	2643.0
23	4762.0
24	7931.0
25	12139.0
26	17866.0
27	24665.0
28	32920.0
29	42428.0
30	53150.0
31	63615.0
32	77472.0
33	93577.0
34	113542.0
35	136202.0
36	175420.0
37	240918.0
38	302596.0
39	303166.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.36734859987715	21.665320835746364	14.753566549533506	22.213764014842983
2	18.986639090308085	24.260149214489534	34.9033783186346	21.849833376567783
3	19.96623613740475	24.85614053168422	33.73827326477111	21.43935006613993
4	13.396043784105688	16.551202376469995	36.3972799185826	33.655473920841715
5	11.800194759984377	39.42700980182615	34.95584522963935	13.816950208550116
6	31.26225667445277	37.54622329003837	12.921792096351734	18.26972793915712
7	24.81948396216527	34.33789510482549	21.41235537197342	19.430265561035824
8	27.188575798583276	34.44698646107757	18.81740987958961	19.547027860749537
9	23.885561234916494	14.447255822451035	22.691236327714414	38.97594661491805
10	16.251274342802166	28.07559451275748	33.24815267871102	22.42497846572933
11	31.61892626700856	22.641755814545867	24.48155255211118	21.257765366334397
12	20.942671116204252	28.26262499685257	31.40156334998884	19.39314053695433
13	32.07297585175287	21.08595965552194	27.215921833671697	19.6251426590535
14	19.694532491130108	23.775298742258055	29.237478941503493	27.292689825108347
15	21.788407272054403	29.7852194387563	27.30053643903093	21.125836850158368
16	20.36085054952648	29.011391058256425	28.075243171835574	22.552515220381522
17	20.839669669265223	27.003419132738355	27.79048135463006	24.36642984336636
18	22.773098762518718	25.23160686356202	30.96724741369164	21.028046960227623
19	22.26898309640268	24.310917977705078	32.81254849236683	20.60755043352542
20	23.46535749231588	23.624514929939693	32.709839829529386	20.200287748215043
21	21.617128572624804	25.044049368084075	33.80912701735566	19.529695041935465
22	19.786700926310342	25.212400226497785	33.33147903402327	21.66941981316861
23	21.314038470659813	25.450609371550637	34.36869599231266	18.86665616547689
24	21.17420478374088	25.525386431096482	31.253180367303514	22.047228417859127
25	20.95245010519733	26.14251676042589	32.556655187577995	20.348377946798788
26	20.706394346221884	27.008162235184102	32.382682874413774	19.902760544180243
27	19.196916632069343	27.172882570738103	32.085155670378974	21.545045126813577
28	19.954524773341188	26.30454348224528	32.21216541364831	21.528766330765226
29	20.33701792365713	26.155984829098994	31.351204484515527	22.15579276272835
30	20.608545899470823	26.388338292120068	33.48156016449782	19.521555643911288
31	21.536847171969082	26.23714458205948	32.910631166399156	19.315377079572276
32	21.042451938025803	26.10919792966506	31.766899351951672	21.081450780357468
33	20.413903028734417	26.125886623455635	32.48515730996994	20.975053037840002
34	19.239370326799758	26.822595671596954	33.32445221558513	20.613581786018152
35	18.835445380247496	29.40395598166703	31.633038460705155	20.12756017738032
36	21.81013185239231	26.449881510274086	31.35518634829714	20.384800289036463
37	20.696615357228808	27.40224963592297	31.300142937198395	20.600992069649823
38	20.407813119421363	25.59957792243915	32.81963386762528	21.172975090514203
39	19.80374096102282	25.349188958760188	33.69476554727497	21.15230453294202
40	18.329221668600017	26.127057759861994	33.16511910750037	22.378601464037622
41	20.400552073701956	25.237696772875072	32.931067496690076	21.430683656732892
42	18.907645939699357	27.700186737699994	31.97770390509579	21.41446341750486
43	19.430031333754552	27.780058240613485	30.808089976067826	21.981820449564133
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	367.0
1	422.0
2	477.0
3	950.5
4	1424.0
5	1424.0
6	2519.0
7	3614.0
8	4064.5
9	4515.0
10	6947.0
11	9379.0
12	9379.0
13	18105.0
14	26831.0
15	41515.5
16	56200.0
17	51812.0
18	47424.0
19	47424.0
20	50111.0
21	52798.0
22	40621.5
23	28445.0
24	30471.5
25	32498.0
26	32498.0
27	34909.0
28	37320.0
29	39833.0
30	42346.0
31	45771.0
32	49196.0
33	49196.0
34	54696.5
35	60197.0
36	66532.0
37	72867.0
38	76633.0
39	80399.0
40	80399.0
41	82565.5
42	84732.0
43	90545.5
44	96359.0
45	106474.0
46	116589.0
47	116589.0
48	163450.5
49	210312.0
50	189325.0
51	168338.0
52	147934.0
53	127530.0
54	127530.0
55	110381.0
56	93232.0
57	76293.5
58	59355.0
59	51705.5
60	44056.0
61	44056.0
62	38463.5
63	32871.0
64	28307.5
65	23744.0
66	19982.0
67	16220.0
68	16220.0
69	13594.5
70	10969.0
71	9088.5
72	7208.0
73	5783.0
74	4358.0
75	4358.0
76	3466.0
77	2574.0
78	2012.0
79	1450.0
80	1100.0
81	750.0
82	750.0
83	597.0
84	444.0
85	307.5
86	171.0
87	138.0
88	105.0
89	105.0
90	75.0
91	45.0
92	33.0
93	21.0
94	13.0
95	5.0
96	5.0
97	6.0
98	7.0
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1707743.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.06545548573828
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.86331096973382	39.34797329330891
2	10.231604990594573	9.835735084461621
3	3.1006787845732413	4.471066142864346
4	1.343356937230574	2.582762522716555
5	0.7424040217040382	1.7841993728824266
6	0.4453132992664551	1.2842511937859427
7	0.32594139057461047	1.0966564979736504
8	0.25158061223773287	0.9673869374870028
9	0.18720859994968778	0.8098439964686195
>10	1.2925905464974892	12.193097295995363
>50	0.12554866202353013	4.19831227023897
>100	0.07750335660703198	7.140311304348998
>500	0.005623208814537335	1.9133621902651368
>1k	0.006234427163943568	5.3535831778595275
>5k	6.112183494062321E-4	1.991508969317238
>10k+	4.889746795249856E-4	5.029949750025713
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	27456	1.6077360586458267	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	26123	1.5296798171621842	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	21108	1.2360173632683606	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10919	0.6393819210501814	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7960	0.46611228972977786	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7071	0.4140552764672436	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6847	0.40093854871605394	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6057	0.35467866066498294	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5959	0.34894009227383743	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4935	0.2889779082683987	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3522	0.20623712115933135	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3431	0.2009084505104105	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3260	0.19089523423606478	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3190	0.186796256813818	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2988	0.1749677791096201	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2763	0.1617924945381126	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2715	0.15898176716285764	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2665	0.1560539261469671	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2519	0.14750463038056663	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2480	0.145220914388172	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2380	0.13936523235639084	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	2197	0.1286493342382314	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2190	0.12823943649600672	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2018	0.11816766340134319	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1979	0.11588394740894854	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	1838	0.10762743574413715	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1788	0.10469959472824658	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.8556820317811284E-5	0.0
3	0.0	0.0	0.0	5.8556820317811284E-5	0.0
4	0.0	0.0	0.0	1.1711364063562257E-4	0.0
5	0.0	0.0	0.0	1.1711364063562257E-4	0.0
6	0.0	0.0	0.0	1.1711364063562257E-4	0.0
7	5.8556820317811284E-5	0.0	0.0	1.1711364063562257E-4	0.0
8	5.8556820317811284E-5	0.0	0.0	1.1711364063562257E-4	0.0
9	5.8556820317811284E-5	0.0	0.0	4.09897742224679E-4	0.0
10	5.8556820317811284E-5	0.0	0.0	7.026818438137354E-4	0.0
11	1.1711364063562257E-4	0.0	0.0	0.0013468068673096595	0.0
12	1.1711364063562257E-4	0.0	0.0	0.0027521705549371305	0.0
13	1.1711364063562257E-4	0.0	0.0	0.003044954656526187	0.0
14	1.7567046095343385E-4	0.0	0.0	0.0036305228597042997	0.0
15	1.7567046095343385E-4	0.0	0.0	0.004743102445742714	0.0
16	1.7567046095343385E-4	0.0	0.0	0.007143932078772977	0.0
17	1.7567046095343385E-4	0.0	0.0	0.009954659454027919	0.0
18	1.7567046095343385E-4	0.0	0.0	0.01136002314165539	0.0
19	1.7567046095343385E-4	0.0	0.0	0.014404977798181576	0.0
20	1.7567046095343385E-4	0.0	0.0	0.02500376227570542	0.0
21	1.7567046095343385E-4	0.0	0.0	0.04227802426945975	0.0
22	1.7567046095343385E-4	0.0	0.0	0.06710611608421173	0.0
23	1.7567046095343385E-4	0.0	0.0	0.08156965070271112	0.0
24	1.7567046095343385E-4	0.0	0.0	0.10358701514220817	0.0
25	1.7567046095343385E-4	0.0	0.0	0.11734786791689382	0.0
26	1.7567046095343385E-4	0.0	0.0	0.13866255051257712	0.0
27	1.7567046095343385E-4	0.0	0.0	0.19271049566591694	0.0
28	1.7567046095343385E-4	0.0	0.0	0.3205985912400168	0.0
29	1.7567046095343385E-4	0.0	0.0	0.490881824724212	0.0
30	1.7567046095343385E-4	0.0	0.0	0.7625269141785386	0.0
31	1.7567046095343385E-4	0.0	0.0	1.0837696304420512	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8195	0.0	24.245274	1
GTATTGG	545	0.0	21.385323	1
ATTGGAC	740	0.0	17.5	3
CGAATTA	75	2.0680073E-4	17.266666	15
GGACGCT	65	0.0015803592	17.076923	31
GTTATGC	80	3.3837697E-4	16.1875	3
CATTGCG	70	0.0025931846	15.857143	29
CAATGCG	105	9.348334E-6	15.857142	19
GTATCAA	13015	0.0	15.280446	2
TAGGACG	85	5.366045E-4	15.235294	4
AATGCGA	135	3.9751285E-7	15.074075	20
TATTGGA	740	0.0	15.0	2
GGACCCT	945	0.0	14.878307	6
TTGGACC	1025	0.0	14.799999	4
CTTACTC	140	5.9992453E-7	14.535715	3
TCTACAC	205	9.276846E-11	14.439025	3
GACCCTC	925	0.0	14.4	7
GTATTGA	180	3.3323886E-9	14.388888	1
TGCGACG	90	8.276916E-4	14.388888	22
TCCGGTC	170	2.4385372E-8	14.147059	8
>>END_MODULE