FastQCFastQC Report
Wed 25 May 2016
SRR2088721_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088721_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2981056
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT401071.345395725541553No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT354461.1890417355460616No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT282520.9477178556860387No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146970.49301321410936255No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG84980.28506676828613753No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG82310.2761102106099315No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA75500.2532659567616308No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA73510.24659046995427125No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG67000.2247525709010498No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA56340.18899343051589773No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA51080.1713486764421735No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC44580.14954432254878808No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT42990.14421064213486764No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38110.12784060413491058No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA37910.12716970093819102No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA37380.12539180746688422No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33560.11257755640954079No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA33220.11143702097511754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100250.024.4513721
GTATTGG8500.019.3705861
ACGTTTA1950.018.9743626
CAATGCG1900.017.52631619
GAACCGT953.6102902E-617.5263166
ATTGGAC9400.017.5159593
GGACCCT12950.016.4285726
TTGGACC14400.015.6736114
TATACCG2650.015.3584915
CTAGACG855.367713E-415.2352944
TAAACGT1954.1836756E-1115.1794874
GTATCAA164150.014.9442592
AGCCGTC2850.014.92982412
ACTAGAC1252.9623916E-614.7999993
GACCCTC14000.014.6678577
CGACGAG2151.2732926E-1114.62790724
TACCGAC6000.014.4916667
TGGACCC15450.014.2491925
TATTGGA11450.014.218342
TTTTTAC18650.013.9865951
Produced by FastQC (version 0.11.5)