##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088721_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2981056
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.504766431761094	31.0	31.0	34.0	30.0	34.0
2	31.714190877326693	31.0	31.0	34.0	30.0	34.0
3	31.78616034049679	31.0	31.0	34.0	30.0	34.0
4	35.519395140513964	37.0	35.0	37.0	33.0	37.0
5	35.39841284430752	37.0	35.0	37.0	33.0	37.0
6	35.44145296163507	37.0	35.0	37.0	33.0	37.0
7	35.29581899836836	37.0	35.0	37.0	32.0	37.0
8	35.335789398119324	37.0	35.0	37.0	32.0	37.0
9	36.913321319693424	39.0	37.0	39.0	33.0	39.0
10	36.73506435303463	39.0	35.0	39.0	32.0	39.0
11	36.82866843158934	39.0	37.0	39.0	32.0	39.0
12	36.67785073477318	39.0	35.0	39.0	32.0	39.0
13	36.72058055937225	39.0	35.0	39.0	32.0	39.0
14	37.655849806243154	39.0	37.0	41.0	32.0	41.0
15	37.68083692490178	39.0	37.0	41.0	32.0	41.0
16	37.5568348934069	39.0	36.0	41.0	32.0	41.0
17	37.617882387986	39.0	36.0	41.0	32.0	41.0
18	37.57842254556775	39.0	36.0	41.0	32.0	41.0
19	37.631554053328756	39.0	37.0	41.0	32.0	41.0
20	37.5175887336568	39.0	36.0	41.0	32.0	41.0
21	37.4433274651667	39.0	36.0	41.0	32.0	41.0
22	37.315526779772	39.0	36.0	41.0	31.0	41.0
23	37.132779122565964	39.0	36.0	41.0	31.0	41.0
24	36.963956732111036	39.0	36.0	41.0	31.0	41.0
25	37.14333477801155	39.0	36.0	41.0	31.0	41.0
26	37.00723871004101	39.0	36.0	41.0	31.0	41.0
27	36.87575912696709	39.0	35.0	41.0	30.0	41.0
28	36.696762154082315	39.0	35.0	41.0	30.0	41.0
29	36.57399156540501	39.0	35.0	40.0	30.0	41.0
30	36.35607382082054	38.0	35.0	40.0	30.0	41.0
31	36.1734492743511	38.0	35.0	40.0	30.0	41.0
32	35.99922544225939	38.0	35.0	40.0	29.0	41.0
33	35.84837017486421	38.0	35.0	40.0	29.0	41.0
34	35.65539895929496	38.0	34.0	40.0	27.0	41.0
35	35.52050917527212	38.0	34.0	40.0	27.0	41.0
36	35.302562246398594	38.0	34.0	40.0	26.0	41.0
37	35.20490960250327	38.0	34.0	40.0	25.0	41.0
38	34.993672376500136	38.0	34.0	40.0	24.0	41.0
39	34.88537518248567	38.0	33.0	40.0	24.0	41.0
40	34.700317605573325	38.0	33.0	40.0	23.0	41.0
41	34.543862309195134	38.0	33.0	40.0	23.0	41.0
42	34.260437576482964	38.0	33.0	40.0	21.0	41.0
43	33.89192588129844	38.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	4.0
14	11.0
15	17.0
16	40.0
17	89.0
18	261.0
19	562.0
20	1277.0
21	2463.0
22	4629.0
23	8112.0
24	13069.0
25	20250.0
26	28901.0
27	39800.0
28	53808.0
29	69382.0
30	86639.0
31	105742.0
32	129979.0
33	158087.0
34	192062.0
35	234102.0
36	299662.0
37	409901.0
38	530611.0
39	591591.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.19023594323622	21.40630702677172	14.3982535047983	23.005203525193757
2	19.105478058781856	23.86889075549067	34.442761222868675	22.582869962858798
3	19.90744219498057	24.273311202473216	33.207024624830936	22.61222197771528
4	13.978033287533009	16.607839638034307	35.81975648897572	33.594370585456964
5	12.581850189999786	38.78712778290646	34.628366592241136	14.002655434852615
6	32.184568153030334	37.264445887631766	13.556605444513622	16.99438051482428
7	25.545679115051843	33.73210701174349	21.25444808819425	19.467765785010414
8	27.288450804010388	34.14742963567273	18.9956847506387	19.56843480967818
9	24.579746237574874	14.554137862556088	22.04779111831512	38.81832478155392
10	16.422972262178234	28.143885924987654	33.23382720753988	22.199314605294234
11	32.93675127203246	22.70628260589536	23.501839616565405	20.855126505506774
12	20.960290581592563	27.66398886837416	30.962148983447474	20.4135715665858
13	31.871927263359023	21.187760310440325	26.2584466712467	20.68186575495395
14	20.31474752570901	23.609083492561027	28.066027609008355	28.010141372721613
15	22.639259376543077	30.018355911462248	25.540144163678914	21.802240548315766
16	21.40140943343567	28.669874031215787	26.929081506687563	22.999635028660983
17	21.435960948066725	27.333669679469292	27.272147856330104	23.95822151613388
18	22.767905064514053	25.88783974537882	29.731746065823657	21.612509124283473
19	22.472003209600892	25.261819972519806	31.179857070782973	21.08631974709633
20	23.525119957491576	24.737609759762982	30.724582161489085	21.01268812125636
21	22.176939983683635	25.690225208785073	31.47351139998712	20.65932340754417
22	20.942243285600807	25.999176130874428	31.031252012709594	22.027328570815175
23	21.95332794821701	26.20383515103373	31.6022577234376	20.240579177311663
24	21.72696520964383	26.21698485368943	29.66187149788531	22.394178438781427
25	21.670609341119388	26.555254245475428	30.696370682067027	21.077765731338157
26	21.34991761308744	27.27895752377681	30.53813816312072	20.832986700015027
27	20.334136628094203	27.26295648253505	30.38128099572769	22.02162589364306
28	20.89870166813371	26.95853415702355	30.275647287404194	21.867116887438545
29	20.948449140170464	26.824890240237014	29.74794837802443	22.47871224156809
30	21.193697803731297	27.189224221215568	31.35291655037678	20.264161424676356
31	22.037257938126622	26.838677300929604	30.884961570664892	20.239103190278882
32	21.54065539191481	26.77775929066747	30.060052545138365	21.62153277227935
33	21.08481021490371	26.67782825951609	30.589160351231243	21.648201174348955
34	20.23444041306168	27.013749490113568	31.35744514695464	21.394364949870113
35	19.927334474763306	28.898517840657806	30.26826735224028	20.905880332338608
36	22.017164387384874	26.696814819983256	30.15102701861354	21.134993774018334
37	21.093297140342216	27.382310161231455	30.208355696773225	21.316037001653104
38	20.84529777367483	26.0182633268211	31.448687981708495	21.687750917795572
39	20.40837206681122	25.667850587174478	32.23726759913266	21.68650974688164
40	19.30315968569527	26.022657742759613	32.032038311256144	22.642144260288973
41	20.583142349556667	25.25927054037227	32.034118111165974	22.123468998905086
42	19.47477672341613	27.061853249318364	31.31537951652032	22.147990510745185
43	19.611238433628888	27.03213223770369	30.732968451448077	22.623660877219347
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1349.0
1	1162.5
2	976.0
3	1771.5
4	2567.0
5	2567.0
6	4026.5
7	5486.0
8	5996.5
9	6507.0
10	10090.0
11	13673.0
12	13673.0
13	25856.0
14	38039.0
15	60242.0
16	82445.0
17	75836.5
18	69228.0
19	69228.0
20	73731.5
21	78235.0
22	63284.5
23	48334.0
24	53644.5
25	58955.0
26	58955.0
27	64427.5
28	69900.0
29	73984.5
30	78069.0
31	84540.5
32	91012.0
33	91012.0
34	100763.0
35	110514.0
36	119619.5
37	128725.0
38	135791.0
39	142857.0
40	142857.0
41	147873.5
42	152890.0
43	160430.5
44	167971.0
45	182611.0
46	197251.0
47	197251.0
48	256021.5
49	314792.0
50	291265.5
51	267739.0
52	241408.0
53	215077.0
54	215077.0
55	191936.0
56	168795.0
57	147336.0
58	125877.0
59	111945.0
60	98013.0
61	98013.0
62	86030.0
63	74047.0
64	64630.5
65	55214.0
66	47414.0
67	39614.0
68	39614.0
69	33894.0
70	28174.0
71	23813.0
72	19452.0
73	16147.0
74	12842.0
75	12842.0
76	10121.5
77	7401.0
78	5818.5
79	4236.0
80	3311.0
81	2386.0
82	2386.0
83	1784.5
84	1183.0
85	909.5
86	636.0
87	479.5
88	323.0
89	323.0
90	233.5
91	144.0
92	116.0
93	88.0
94	57.0
95	26.0
96	26.0
97	17.5
98	9.0
99	7.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2981056.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.88010324742523
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.31223455062849	35.77525434775195
2	12.800157630284382	12.001454225820991
3	4.617727941583736	6.494386880098979
4	2.093739607690961	3.926189159271033
5	1.0834396259392238	2.5395880763191294
6	0.6546644743130756	1.8414442888931009
7	0.42990844846364473	1.4107906715641236
8	0.28811491948177514	1.080548573794338
9	0.22161040826998443	0.9350206938360835
>10	1.2777086401040711	11.323101171228393
>50	0.13089722702991316	4.236525270443142
>100	0.0791033642943792	6.968858028738239
>500	0.005167165489819122	1.6166645023218154
>1k	0.004736568365642494	4.2123039419557635
>5k	5.023633115075372E-4	1.650981446300005
>10k+	2.870647494328784E-4	3.9868887216629276
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	40107	1.345395725541553	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	35446	1.1890417355460616	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	28252	0.9477178556860387	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14697	0.49301321410936255	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	8498	0.28506676828613753	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8231	0.2761102106099315	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7550	0.2532659567616308	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7351	0.24659046995427125	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6700	0.2247525709010498	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5634	0.18899343051589773	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	5108	0.1713486764421735	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4458	0.14954432254878808	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4299	0.14421064213486764	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3811	0.12784060413491058	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3791	0.12716970093819102	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3738	0.12539180746688422	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3356	0.11257755640954079	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3322	0.11143702097511754	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	3.354515983597759E-5	0.0	0.0	0.0	0.0
6	3.354515983597759E-5	0.0	0.0	0.0	0.0
7	3.354515983597759E-5	0.0	0.0	0.0	0.0
8	3.354515983597759E-5	0.0	0.0	3.354515983597759E-5	0.0
9	3.354515983597759E-5	0.0	0.0	1.0063547950793276E-4	0.0
10	6.709031967195518E-5	0.0	0.0	2.683612786878207E-4	0.0
11	6.709031967195518E-5	0.0	0.0	3.019064385237983E-4	0.0
12	6.709031967195518E-5	0.0	0.0	4.696322377036862E-4	0.0
13	1.0063547950793276E-4	0.0	0.0	6.038128770475966E-4	0.0
14	1.0063547950793276E-4	0.0	0.0	6.709031967195517E-4	0.0
15	1.0063547950793276E-4	0.0	0.0	9.057193155713948E-4	0.0
16	1.0063547950793276E-4	0.0	0.0	0.0012411709139311708	0.0
17	1.3418063934391035E-4	0.0	0.0	0.0018114386311427896	0.0
18	1.3418063934391035E-4	0.0	0.0	0.0021804353893385433	0.0
19	2.0127095901586553E-4	0.0	0.0	0.0029519740655660274	0.0
20	2.0127095901586553E-4	0.0	0.0	0.004461506258185019	0.0
21	2.0127095901586553E-4	0.0	0.0	0.007111573885227249	0.0
22	2.0127095901586553E-4	0.0	0.0	0.01153953498357629	0.0
23	2.0127095901586553E-4	0.0	0.0	0.014625689688486227	0.0
24	2.0127095901586553E-4	0.0	0.0	0.02016064106142253	0.0
25	2.0127095901586553E-4	0.0	0.0	0.024823418278623412	0.0
26	2.0127095901586553E-4	0.0	0.0	0.03488696622941669	0.0
27	2.0127095901586553E-4	0.0	0.0	0.095804976491552	0.0
28	2.0127095901586553E-4	0.0	0.0	0.2173055454174628	0.0
29	2.0127095901586553E-4	0.0	0.0	0.3754374288842611	0.0
30	2.0127095901586553E-4	0.0	0.0	0.6071673930311943	0.0
31	2.0127095901586553E-4	0.0	0.0	0.8886783743747182	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10025	0.0	24.451372	1
GTATTGG	850	0.0	19.370586	1
ACGTTTA	195	0.0	18.97436	26
CAATGCG	190	0.0	17.526316	19
GAACCGT	95	3.6102902E-6	17.526316	6
ATTGGAC	940	0.0	17.515959	3
GGACCCT	1295	0.0	16.428572	6
TTGGACC	1440	0.0	15.673611	4
TATACCG	265	0.0	15.358491	5
CTAGACG	85	5.367713E-4	15.235294	4
TAAACGT	195	4.1836756E-11	15.179487	4
GTATCAA	16415	0.0	14.944259	2
AGCCGTC	285	0.0	14.929824	12
ACTAGAC	125	2.9623916E-6	14.799999	3
GACCCTC	1400	0.0	14.667857	7
CGACGAG	215	1.2732926E-11	14.627907	24
TACCGAC	600	0.0	14.491666	7
TGGACCC	1545	0.0	14.249192	5
TATTGGA	1145	0.0	14.21834	2
TTTTTAC	1865	0.0	13.986595	1
>>END_MODULE