FastQCFastQC Report
Wed 25 May 2016
SRR2088720_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088720_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1755326
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT207721.1833699267258617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT186571.0628794879127867No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT142850.8138089448911484No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78950.44977400209419793No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG41890.23864512916688982No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG39390.224402760512862No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA38190.21756642355892866No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA34670.1975131684940575No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG33360.19005016731934696No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA28520.16247694160514914No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA24350.13872067069023075No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23060.1313716084647524No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC22010.12538981363006074No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21040.11986377459229797No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA18600.10596322278596682No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18070.10294384063131293No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA17590.10020930584973958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57100.025.1742551
TGCGACG1258.587449E-917.76000222
CTAACGC953.6085785E-617.5263163
TAACGCC852.7244407E-517.4117644
CAATGCG1401.87174E-917.17857219
GTATCAA86950.016.489362
ACGCGAA904.4481058E-516.44444534
CGACGAG1352.2213499E-816.44444524
GACGCGA904.4481058E-516.44444533
TATACCG1352.2213499E-816.4444455
CTAATAC4100.015.7926843
TGACGCG957.063066E-515.57894832
ACGACCC957.063066E-515.57894830
GTATTAG2750.015.4727281
AAGACCG1455.3501935E-815.3103465
TATACGG1455.3501935E-815.3103462
ACGTTTA855.3661526E-415.23529426
CAGACGT855.3661526E-415.2352944
GTATTGG3700.015.01
TAATACT4000.014.84
Produced by FastQC (version 0.11.5)