##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088720_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1755326
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.505940776812967	31.0	31.0	34.0	30.0	34.0
2	31.71977456039505	31.0	31.0	34.0	30.0	34.0
3	31.792685803092986	31.0	31.0	34.0	30.0	34.0
4	35.528457961654986	37.0	35.0	37.0	33.0	37.0
5	35.399354877669445	37.0	35.0	37.0	33.0	37.0
6	35.443040210194575	37.0	35.0	37.0	33.0	37.0
7	35.304953040062074	37.0	35.0	37.0	32.0	37.0
8	35.336009949149044	37.0	35.0	37.0	32.0	37.0
9	36.92656008057762	39.0	37.0	39.0	33.0	39.0
10	36.730773656859185	39.0	35.0	39.0	32.0	39.0
11	36.83273876191659	39.0	37.0	39.0	32.0	39.0
12	36.67590806494064	39.0	35.0	39.0	32.0	39.0
13	36.715206178225586	39.0	35.0	39.0	32.0	39.0
14	37.67968685019193	39.0	37.0	41.0	32.0	41.0
15	37.69462766460475	39.0	37.0	41.0	32.0	41.0
16	37.58159737849265	39.0	36.0	41.0	32.0	41.0
17	37.62463154992292	39.0	36.0	41.0	32.0	41.0
18	37.58194831045629	39.0	36.0	41.0	32.0	41.0
19	37.639512546387394	39.0	37.0	41.0	32.0	41.0
20	37.52398528820287	39.0	36.0	41.0	32.0	41.0
21	37.4545377895616	39.0	36.0	41.0	32.0	41.0
22	37.31994626639154	39.0	36.0	41.0	31.0	41.0
23	37.13959970968356	39.0	36.0	41.0	31.0	41.0
24	36.9745790810368	39.0	36.0	41.0	31.0	41.0
25	37.15741121592229	39.0	36.0	41.0	31.0	41.0
26	37.02875989987045	39.0	36.0	41.0	31.0	41.0
27	36.911509315078796	39.0	36.0	41.0	31.0	41.0
28	36.73647060432079	39.0	35.0	41.0	30.0	41.0
29	36.62564617626583	39.0	35.0	40.0	30.0	41.0
30	36.41818898597754	39.0	35.0	40.0	30.0	41.0
31	36.26036189289055	38.0	35.0	40.0	30.0	41.0
32	36.07840936669314	38.0	35.0	40.0	30.0	41.0
33	35.946529020820066	38.0	35.0	40.0	29.0	41.0
34	35.76474797274125	38.0	35.0	40.0	28.0	41.0
35	35.631662152785296	38.0	34.0	40.0	27.0	41.0
36	35.438825038767725	38.0	34.0	40.0	26.0	41.0
37	35.35737008396161	38.0	34.0	40.0	26.0	41.0
38	35.16222513652735	38.0	34.0	40.0	25.0	41.0
39	35.06676993333432	38.0	34.0	40.0	24.0	41.0
40	34.90643618336423	38.0	34.0	40.0	24.0	41.0
41	34.775536851844045	38.0	33.0	40.0	23.0	41.0
42	34.511502136924996	38.0	33.0	40.0	22.0	41.0
43	34.15972018872848	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	3.0
14	5.0
15	7.0
16	31.0
17	62.0
18	127.0
19	305.0
20	736.0
21	1457.0
22	2672.0
23	4529.0
24	7244.0
25	11161.0
26	16057.0
27	22740.0
28	30537.0
29	39149.0
30	49978.0
31	61076.0
32	75379.0
33	92092.0
34	112570.0
35	137736.0
36	176787.0
37	241114.0
38	319884.0
39	351886.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.5708683173382	21.8281960160107	14.335057989228211	23.265877677422882
2	18.959270243817958	23.70380202879693	34.81330533473554	22.52362239264957
3	19.634073670645794	24.593380374927506	32.85748630168983	22.915059652736872
4	13.991190240445365	16.92249758734275	36.0819015954871	33.00441057672478
5	12.551457677947003	38.79638312199557	34.91283100688989	13.739328193167538
6	32.01342656577752	37.60258778141497	13.928637757316874	16.45534789549064
7	25.50466409088682	33.54499392135706	21.762339303354477	19.188002684401646
8	26.587539864389864	34.81022898310627	19.29630165564687	19.305929496856994
9	24.586601007448188	14.683483295980349	22.046104256417326	38.68381144015414
10	16.24718143524337	28.34464936997458	33.677049163517204	21.731120031264847
11	32.8149301041516	23.005641117376488	23.465270838579272	20.714157939892647
12	20.667101153859736	27.650875108099576	31.078614456801755	20.60340928123893
13	30.987748144789055	21.65124882785306	26.265149607537293	21.095853419820592
14	20.14469107163	23.564226815987457	28.14468651407203	28.146395598310512
15	22.397264097951037	30.243271050505715	25.54044092094574	21.819023930597506
16	21.398817085829073	28.78792885196254	27.027515116850086	22.785738945358297
17	21.2641982173112	27.64044969424483	27.434618982456822	23.66073310598715
18	22.29112996674122	26.53000069502759	29.75264993511177	21.426219403119422
19	22.27153246747328	25.772135774209463	31.06539753869082	20.890934219626438
20	23.037145236839194	25.478287224139564	30.64587432761778	20.838693211403466
21	21.9993323177575	26.139531916008764	31.14697782634109	20.714157939892647
22	20.924318331751483	26.591299849714527	30.656641558320217	21.827740260213773
23	21.81503606737438	26.68176737540491	31.14486995578029	20.358326601440417
24	21.63472768021439	26.779583963320775	29.38251925853089	22.203169097933944
25	21.556850408414164	27.077990071359963	30.368888742034244	20.99627077819163
26	21.211501453291298	27.58108750169484	30.22612323864627	20.981287806367593
27	20.394217370448565	27.537391914664283	30.140441148823637	21.92794956606351
28	20.83601564609651	27.261146932250764	30.166077412400888	21.736760009251842
29	21.032560333522092	27.018798787233823	29.789623124137627	22.159017755106458
30	21.18626397603636	27.44686741949928	30.969574882386517	20.397293722077837
31	21.85269289009563	27.172787277121174	30.651343397180923	20.323176435602274
32	21.36907902007946	27.157690366347904	29.78455284089679	21.688677772675845
33	20.97895205790833	26.915399190805584	30.41041948902939	21.6952292622567
34	20.283297803371	27.086877309400077	31.193180070254755	21.43664481697417
35	19.999134063985835	28.603290784731726	30.297392051391025	21.100183099891417
36	21.853319554316407	26.669404999413217	30.130756338138898	21.34651910813148
37	21.091580709224385	27.240239135066645	30.232845636650968	21.435334519058
38	20.778476476734237	26.065129782160124	31.449257858654178	21.707135882451464
39	20.218865327580176	25.772477591057157	32.245634144312795	21.76302293704987
40	19.29482044930685	26.128593776882468	32.01604716160987	22.560538612200812
41	20.363055067833553	25.358480419021877	32.06333182554123	22.21513268760333
42	19.250555167530134	27.037256897009442	31.470963228482912	22.24122470697751
43	19.529876501572925	26.861221220445664	30.921606584759754	22.687295693221657
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	850.0
1	736.0
2	622.0
3	1067.5
4	1513.0
5	1513.0
6	2331.0
7	3149.0
8	3342.5
9	3536.0
10	5706.0
11	7876.0
12	7876.0
13	14408.5
14	20941.0
15	32360.5
16	43780.0
17	40317.5
18	36855.0
19	36855.0
20	40106.5
21	43358.0
22	35906.0
23	28454.0
24	31763.0
25	35072.0
26	35072.0
27	39160.0
28	43248.0
29	46455.5
30	49663.0
31	54436.0
32	59209.0
33	59209.0
34	65222.0
35	71235.0
36	77258.0
37	83281.0
38	87606.5
39	91932.0
40	91932.0
41	95185.0
42	98438.0
43	102928.5
44	107419.0
45	113117.0
46	118815.0
47	118815.0
48	146677.5
49	174540.0
50	161202.5
51	147865.0
52	134086.0
53	120307.0
54	120307.0
55	108385.5
56	96464.0
57	83700.0
58	70936.0
59	63765.5
60	56595.0
61	56595.0
62	49751.5
63	42908.0
64	37385.5
65	31863.0
66	27147.0
67	22431.0
68	22431.0
69	18933.0
70	15435.0
71	13044.0
72	10653.0
73	8692.0
74	6731.0
75	6731.0
76	5473.5
77	4216.0
78	3291.5
79	2367.0
80	1862.0
81	1357.0
82	1357.0
83	1036.0
84	715.0
85	536.0
86	357.0
87	267.5
88	178.0
89	178.0
90	132.0
91	86.0
92	65.5
93	45.0
94	31.0
95	17.0
96	17.0
97	13.5
98	10.0
99	7.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1755326.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.22787593001627
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.8096738200328	42.48099416107773
2	11.624034815941299	12.374453659782258
3	3.634132750866907	5.803115015290574
4	1.5239039965388432	3.2445669142810196
5	0.815556150105424	2.170516078588662
6	0.4999464821868191	1.5966653595292857
7	0.3400214772111835	1.266903470177641
8	0.25575468320105427	1.0890622836757065
9	0.17842933314410223	0.8547672966182831
>10	1.1554988752362743	11.564728793303809
>50	0.10051742749787523	3.708184709497235
>100	0.05416460789524888	5.305018524175597
>500	0.004504543153453478	1.6418348846016502
>1k	0.003432032878821698	3.3820885223692683
>5k	1.0725102746317806E-4	0.450705369051809
>10k+	3.2175308238953414E-4	3.066394957979591
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	20772	1.1833699267258617	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18657	1.0628794879127867	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	14285	0.8138089448911484	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7895	0.44977400209419793	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4189	0.23864512916688982	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3939	0.224402760512862	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3819	0.21756642355892866	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3467	0.1975131684940575	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3336	0.19005016731934696	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2852	0.16247694160514914	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2435	0.13872067069023075	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2306	0.1313716084647524	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2201	0.12538981363006074	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2104	0.11986377459229797	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1860	0.10596322278596682	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1807	0.10294384063131293	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1759	0.10020930584973958	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	5.696947461611119E-5	0.0	0.0	0.0	0.0
9	1.1393894923222239E-4	0.0	0.0	2.84847373080556E-4	0.0
10	1.1393894923222239E-4	0.0	0.0	2.84847373080556E-4	0.0
11	1.1393894923222239E-4	0.0	0.0	5.69694746161112E-4	0.0
12	1.1393894923222239E-4	0.0	0.0	7.406031700094456E-4	0.0
13	1.1393894923222239E-4	0.0	0.0	7.406031700094456E-4	0.0
14	2.2787789846444477E-4	0.0	0.0	0.0010254505430900016	0.0
15	2.84847373080556E-4	0.0	0.0	0.0014812063400188912	0.0
16	2.84847373080556E-4	0.0	0.0	0.0018799926623316694	0.0
17	2.84847373080556E-4	0.0	0.0	0.0023927179338766704	0.0
18	3.418168476966672E-4	0.0	0.0	0.0027345347815733376	0.0
19	3.418168476966672E-4	0.0	0.0	0.0034181684769666715	0.0
20	3.418168476966672E-4	0.0	0.0	0.0054121000885305635	0.0
21	3.418168476966672E-4	0.0	0.0	0.008203604344720013	0.0
22	3.9878632231277834E-4	0.0	0.0	0.013046009687089464	0.0
23	3.9878632231277834E-4	0.0	0.0	0.017261750808681694	0.0
24	3.9878632231277834E-4	0.0	0.0	0.023984148813382813	0.0
25	3.9878632231277834E-4	0.0	0.0	0.029339279427297266	0.0
26	3.9878632231277834E-4	0.0	0.0	0.040391357502822835	0.0
27	3.9878632231277834E-4	0.0	0.0	0.10385535222517071	0.0
28	3.9878632231277834E-4	0.0	0.0	0.22765002056598033	0.0
29	3.9878632231277834E-4	0.0	0.0	0.3900699926965134	0.0
30	3.9878632231277834E-4	0.0	0.0	0.649622919047516	0.0
31	3.9878632231277834E-4	0.0	0.0	0.977539214937852	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5710	0.0	25.174255	1
TGCGACG	125	8.587449E-9	17.760002	22
CTAACGC	95	3.6085785E-6	17.526316	3
TAACGCC	85	2.7244407E-5	17.411764	4
CAATGCG	140	1.87174E-9	17.178572	19
GTATCAA	8695	0.0	16.48936	2
ACGCGAA	90	4.4481058E-5	16.444445	34
CGACGAG	135	2.2213499E-8	16.444445	24
GACGCGA	90	4.4481058E-5	16.444445	33
TATACCG	135	2.2213499E-8	16.444445	5
CTAATAC	410	0.0	15.792684	3
TGACGCG	95	7.063066E-5	15.578948	32
ACGACCC	95	7.063066E-5	15.578948	30
GTATTAG	275	0.0	15.472728	1
AAGACCG	145	5.3501935E-8	15.310346	5
TATACGG	145	5.3501935E-8	15.310346	2
ACGTTTA	85	5.3661526E-4	15.235294	26
CAGACGT	85	5.3661526E-4	15.235294	4
GTATTGG	370	0.0	15.0	1
TAATACT	400	0.0	14.8	4
>>END_MODULE