FastQCFastQC Report
Wed 25 May 2016
SRR2088719_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088719_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2852262
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT606212.1253657623317914No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT554721.9448423742278935No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT453871.5912633551896704No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223310.7829224664494356No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG166070.5822396399769727No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG164570.5769806560547384No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA153210.5371526178170168No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG134840.47274759471605343No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA121860.42723985384231883No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA96130.33703074962959223No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA83460.2926098654331194No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC76480.2681380602483222No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA67390.23626861767958202No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA65910.23107975354297747No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT63930.22413789476562812No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA57890.20296171950543113No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54570.19132183509088577No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52900.1854668329907982No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG52390.1836787784572385No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA50050.17547476353855293No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG48560.17025083950913344No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT48230.16909386304624188No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG48010.16832254540431418No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC45280.15875119466584767No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA44320.15538544495561768No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT40500.14199256590032755No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT37510.13150965794867372No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC37460.13133435848459923No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG37180.13035268148578216No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA36750.12884510609474165No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG36570.12821402802407353No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT36380.1275478900605905No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA33690.11811677889338357No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT33410.11713510189456648No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG30460.10679243351417225No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC30340.10637171480039351No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA29700.10412788166024019No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT29370.10297090519734863No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29320.10279560573327415No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAAAAAAA29030.10177886884164217No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA28890.10128803034223363No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28770.10086731162845489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA167950.024.1342661
GTATTGG9250.024.01
ATTGGAC10450.021.775123
TTGGACC16950.019.8643054
GGACCCT16950.018.8820046
TATTGGA11500.018.821742
TGGACCC17700.018.709045
TAGGTCG1409.458745E-1118.521
TTTTTAC24100.017.425311
GACCCTC18950.016.5963067
GTCGTCA1708.54925E-1116.3235324
TTCGTTA3250.015.93846130
CTGTGCG2500.015.5400019
CGCCGTT1557.221388E-915.51612925
ACTCTGT16050.015.44548337
ATTAGAC2650.015.3584893
AGTGCGC1701.4897523E-915.2352958
GTATCAA268150.015.1366782
CCGTTTA1252.9622825E-614.80000127
TACATCG750.004106388414.85
Produced by FastQC (version 0.11.5)