##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088719_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2852262
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.422878052577218	31.0	31.0	34.0	30.0	34.0
2	31.61197077968293	31.0	31.0	34.0	30.0	34.0
3	31.652851315902957	31.0	31.0	34.0	30.0	34.0
4	35.38495587011291	37.0	35.0	37.0	33.0	37.0
5	35.306172434369635	37.0	35.0	37.0	33.0	37.0
6	35.388588425607466	37.0	35.0	37.0	33.0	37.0
7	35.21085264958128	37.0	35.0	37.0	32.0	37.0
8	35.29798910478771	37.0	35.0	37.0	32.0	37.0
9	36.82972076197769	39.0	37.0	39.0	32.0	39.0
10	36.68823761631996	39.0	35.0	39.0	32.0	39.0
11	36.778954738379575	39.0	37.0	39.0	32.0	39.0
12	36.613773909970405	39.0	35.0	39.0	32.0	39.0
13	36.67063509593439	39.0	35.0	39.0	32.0	39.0
14	37.50673745960224	39.0	36.0	41.0	32.0	41.0
15	37.52567015232121	39.0	36.0	41.0	32.0	41.0
16	37.3795377142773	39.0	36.0	41.0	32.0	41.0
17	37.54625276359605	39.0	36.0	41.0	32.0	41.0
18	37.53525377402216	39.0	36.0	41.0	32.0	41.0
19	37.62284635843411	39.0	37.0	41.0	32.0	41.0
20	37.52450651447868	39.0	36.0	41.0	32.0	41.0
21	37.464299913542305	39.0	36.0	41.0	32.0	41.0
22	37.35454807447562	39.0	36.0	41.0	31.0	41.0
23	37.17157890824896	39.0	36.0	41.0	31.0	41.0
24	36.99100573509727	39.0	36.0	41.0	31.0	41.0
25	37.14192209551577	39.0	36.0	41.0	31.0	41.0
26	36.967765583947056	39.0	36.0	41.0	31.0	41.0
27	36.83144956529239	39.0	35.0	41.0	31.0	41.0
28	36.62907580018946	39.0	35.0	40.0	30.0	41.0
29	36.49057625141028	39.0	35.0	40.0	30.0	41.0
30	36.2211732302292	38.0	35.0	40.0	30.0	41.0
31	35.95970601578677	38.0	35.0	40.0	30.0	41.0
32	35.76390913597699	38.0	35.0	40.0	29.0	41.0
33	35.563359186498296	38.0	34.0	40.0	28.0	41.0
34	35.350128073788454	38.0	34.0	40.0	27.0	41.0
35	35.15043744228265	38.0	34.0	40.0	26.0	41.0
36	34.8909416456132	38.0	33.0	40.0	25.0	41.0
37	34.72347456159357	38.0	33.0	40.0	24.0	41.0
38	34.45448349415306	38.0	33.0	40.0	23.0	41.0
39	34.28504253816795	38.0	33.0	40.0	22.0	41.0
40	34.01751311765889	37.0	33.0	40.0	20.0	41.0
41	33.78824876536587	37.0	33.0	40.0	18.0	41.0
42	33.40843653212784	37.0	33.0	40.0	15.0	41.0
43	32.991794232086676	37.0	32.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	1.0
12	2.0
13	1.0
14	10.0
15	14.0
16	37.0
17	79.0
18	230.0
19	583.0
20	1252.0
21	2542.0
22	4771.0
23	8127.0
24	13569.0
25	20680.0
26	30944.0
27	42485.0
28	56878.0
29	73247.0
30	89492.0
31	108435.0
32	130548.0
33	157769.0
34	190408.0
35	229358.0
36	293727.0
37	408796.0
38	488234.0
39	500041.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.685581478840305	21.556014138953575	15.617604553859357	21.140799828346765
2	19.110481435436157	24.857148466725707	34.767458248926644	21.264911848911495
3	20.27913284263507	24.37777455226764	34.718128979736086	20.624963625361204
4	13.213863242577295	16.44820847453705	35.59455618032285	34.74337210256281
5	11.470159473428458	39.87046070802752	34.33152354166623	14.32785627687779
6	31.217118203026228	37.02086273981843	12.135000220877325	19.627018836278012
7	24.159526719494913	35.25773579005014	20.46617737080254	20.1165601196524
8	28.045670418776396	33.856532113810026	18.128944676190336	19.968852791223245
9	23.633172548664884	14.361829313015424	23.015206877909534	38.98979126041016
10	16.65302836836167	28.45453888878371	32.16878393359376	22.72364880926086
11	31.317634915726533	22.37883476342636	24.599878973250004	21.7036513475971
12	21.473167612231975	28.705076882838952	31.04157331970205	18.780182185227023
13	33.411096175596775	20.91518941808291	27.323156147647026	18.350558258673292
14	19.504239091640247	24.351199153513946	29.80038299426911	26.344178760576693
15	21.42134909065156	29.900268628898747	28.046897515024916	20.631484765424776
16	19.780581166807256	28.9737408414795	28.711983681723492	22.533694309989755
17	20.752371275850535	26.705085297213227	27.757197620695433	24.7853458062408
18	23.060539319319194	24.371148232525623	31.792871762832448	20.77544068532274
19	22.12160032984347	23.567715728779472	34.1612727021571	20.14941123921996
20	23.577812977910163	22.506803372200732	34.01170719940875	19.903676450480358
21	21.40658887577649	24.173340317263982	35.59220716750425	18.82786363945528
22	19.01992173229528	24.460025060811383	34.930486750515904	21.58956645637743
23	20.98099683689647	24.727602162774666	36.192748071530595	18.098652928798266
24	20.612797842554436	24.769989573187875	32.60317600557032	22.01403657868737
25	20.445772513184274	25.74444423408509	34.016124745903426	19.793658506827214
26	20.089809421434637	26.740565908741903	33.79710559548877	19.372519074334686
27	18.247096514976533	26.86877993676598	33.48300401576012	21.401119532497365
28	19.24707477784299	25.99624438428167	33.37133825714468	21.38534258073066
29	19.50210043817854	25.685964332869844	32.4211801019682	22.390755126983425
30	19.93901682243777	26.070886896084584	35.20921289839433	18.78088338308332
31	21.156506660327835	25.92608953875906	34.355013669852205	18.56239013106089
32	20.820036868983287	25.668925224961804	32.90097473514004	20.610063170914874
33	19.90563980447799	25.653113213302287	33.843104174861914	20.598142807357807
34	18.239067799521923	26.78908880039772	34.88708260321107	20.08476079686929
35	17.794508358629045	29.90882324274558	32.86966625085634	19.427002147769034
36	21.395895608467946	26.28327972675722	32.56527626143742	19.75554840333742
37	20.222651355310276	27.87976700597631	32.200828675626575	19.69675296308684
38	19.891650907244845	25.339081753359267	34.2041158911769	20.565151448218995
39	19.12569742891782	25.076413036390065	35.10336708198616	20.694522452705957
40	17.3968590543225	25.862841492120992	34.44038450885648	22.29991494470003
41	19.872017367268505	24.89886973917543	34.28966202964524	20.939450863910817
42	18.258175441106044	27.88660368507521	33.03781349679657	20.817407377022167
43	18.872144284080495	28.175602381548398	31.500507316649028	21.451746017722076
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	714.0
1	780.5
2	847.0
3	1722.5
4	2598.0
5	2598.0
6	4549.5
7	6501.0
8	7437.5
9	8374.0
10	13517.5
11	18661.0
12	18661.0
13	36521.0
14	54381.0
15	86920.0
16	119459.0
17	108924.0
18	98389.0
19	98389.0
20	101828.0
21	105267.0
22	78182.5
23	51098.0
24	53599.0
25	56100.0
26	56100.0
27	58974.0
28	61848.0
29	62776.0
30	63704.0
31	67072.5
32	70441.0
33	70441.0
34	78032.5
35	85624.0
36	94719.5
37	103815.0
38	109296.5
39	114778.0
40	114778.0
41	118024.5
42	121271.0
43	130987.0
44	140703.0
45	163987.0
46	187271.0
47	187271.0
48	289477.0
49	391683.0
50	346958.5
51	302234.0
52	261180.0
53	220126.0
54	220126.0
55	185911.5
56	151697.0
57	121834.0
58	91971.0
59	79927.0
60	67883.0
61	67883.0
62	58334.0
63	48785.0
64	42412.5
65	36040.0
66	30572.0
67	25104.0
68	25104.0
69	21112.5
70	17121.0
71	14303.5
72	11486.0
73	9416.0
74	7346.0
75	7346.0
76	5782.5
77	4219.0
78	3264.0
79	2309.0
80	1759.5
81	1210.0
82	1210.0
83	908.0
84	606.0
85	455.5
86	305.0
87	227.5
88	150.0
89	150.0
90	119.0
91	88.0
92	56.0
93	24.0
94	22.0
95	20.0
96	20.0
97	15.0
98	10.0
99	5.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2852262.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.74693374813567
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.37619869591381	29.980904445531536
2	12.05203854915207	9.339590783879446
3	4.110317961603821	4.777866532261059
4	1.9263686183974844	2.9856350892613985
5	1.038220353006802	2.011392761696029
6	0.6277610318650745	1.4594289066802393
7	0.43259823513420015	1.1733298609413685
8	0.31585821116528445	0.9790829745460737
9	0.2311983066537495	0.8062402923534585
>10	1.572911603588786	11.825959689155336
>50	0.18031153829781357	4.8407648853864105
>100	0.11505222861050246	8.340763515183653
>500	0.011172763918930639	3.001124558304251
>1k	0.0081751931114126	6.86409265950443
>5k	9.99190269172651E-4	2.537980882845094
>10k+	8.175193111412601E-4	9.075842162470295
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	60621	2.1253657623317914	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	55472	1.9448423742278935	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	45387	1.5912633551896704	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22331	0.7829224664494356	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	16607	0.5822396399769727	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	16457	0.5769806560547384	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	15321	0.5371526178170168	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	13484	0.47274759471605343	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	12186	0.42723985384231883	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9613	0.33703074962959223	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	8346	0.2926098654331194	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	7648	0.2681380602483222	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6739	0.23626861767958202	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6591	0.23107975354297747	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6393	0.22413789476562812	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5789	0.20296171950543113	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5457	0.19132183509088577	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5290	0.1854668329907982	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	5239	0.1836787784572385	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5005	0.17547476353855293	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4856	0.17025083950913344	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	4823	0.16909386304624188	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4801	0.16832254540431418	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	4528	0.15875119466584767	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4432	0.15538544495561768	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	4050	0.14199256590032755	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	3751	0.13150965794867372	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3746	0.13133435848459923	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	3718	0.13035268148578216	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	3675	0.12884510609474165	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	3657	0.12821402802407353	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3638	0.1275478900605905	No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA	3369	0.11811677889338357	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	3341	0.11713510189456648	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	3046	0.10679243351417225	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	3034	0.10637171480039351	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	2970	0.10412788166024019	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT	2937	0.10297090519734863	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2932	0.10279560573327415	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAAAAAAA	2903	0.10177886884164217	No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA	2889	0.10128803034223363	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2877	0.10086731162845489	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	3.505989281489569E-5	0.0	0.0	0.0	3.505989281489569E-5
7	3.505989281489569E-5	0.0	0.0	0.0	7.011978562979138E-5
8	3.505989281489569E-5	0.0	0.0	0.0	7.011978562979138E-5
9	3.505989281489569E-5	0.0	0.0	1.7529946407447843E-4	7.011978562979138E-5
10	3.505989281489569E-5	0.0	0.0	5.258983922234353E-4	7.011978562979138E-5
11	3.505989281489569E-5	0.0	0.0	0.001682874855114993	7.011978562979138E-5
12	3.505989281489569E-5	0.0	0.0	0.0025944320683022807	7.011978562979138E-5
13	3.505989281489569E-5	0.0	0.0	0.002804791425191655	7.011978562979138E-5
14	3.505989281489569E-5	0.0	0.0	0.0032255101389704033	7.011978562979138E-5
15	3.505989281489569E-5	0.0	0.0	0.004417546494676856	7.011978562979138E-5
16	3.505989281489569E-5	0.0	0.0	0.00641596038512591	7.011978562979138E-5
17	3.505989281489569E-5	0.0	0.0	0.00869485341809413	7.011978562979138E-5
18	3.505989281489569E-5	0.0	0.0	0.010132309023504853	7.011978562979138E-5
19	3.505989281489569E-5	0.0	0.0	0.012235902592398595	7.011978562979138E-5
20	3.505989281489569E-5	0.0	0.0	0.022227972044643864	7.011978562979138E-5
21	3.505989281489569E-5	0.0	0.0	0.03793480402571713	1.4023957125958275E-4
22	3.505989281489569E-5	0.0	0.0	0.06167035146140151	1.4023957125958275E-4
23	3.505989281489569E-5	0.0	0.0	0.073941313946615	1.4023957125958275E-4
24	3.505989281489569E-5	0.0	0.0	0.09171667960376712	1.4023957125958275E-4
25	3.505989281489569E-5	0.0	0.0	0.10100755119971447	1.4023957125958275E-4
26	3.505989281489569E-5	0.0	0.0	0.11527692757537701	1.4023957125958275E-4
27	3.505989281489569E-5	0.0	0.0	0.18325805974345974	1.4023957125958275E-4
28	3.505989281489569E-5	0.0	0.0	0.3315964662432834	1.4023957125958275E-4
29	3.505989281489569E-5	0.0	0.0	0.5372928573882764	1.4023957125958275E-4
30	7.011978562979138E-5	0.0	0.0	0.8683283653465215	1.4023957125958275E-4
31	7.011978562979138E-5	0.0	0.0	1.2185065747816997	1.4023957125958275E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16795	0.0	24.134266	1
GTATTGG	925	0.0	24.0	1
ATTGGAC	1045	0.0	21.77512	3
TTGGACC	1695	0.0	19.864305	4
GGACCCT	1695	0.0	18.882004	6
TATTGGA	1150	0.0	18.82174	2
TGGACCC	1770	0.0	18.70904	5
TAGGTCG	140	9.458745E-11	18.5	21
TTTTTAC	2410	0.0	17.42531	1
GACCCTC	1895	0.0	16.596306	7
GTCGTCA	170	8.54925E-11	16.32353	24
TTCGTTA	325	0.0	15.938461	30
CTGTGCG	250	0.0	15.540001	9
CGCCGTT	155	7.221388E-9	15.516129	25
ACTCTGT	1605	0.0	15.445483	37
ATTAGAC	265	0.0	15.358489	3
AGTGCGC	170	1.4897523E-9	15.235295	8
GTATCAA	26815	0.0	15.136678	2
CCGTTTA	125	2.9622825E-6	14.800001	27
TACATCG	75	0.0041063884	14.8	5
>>END_MODULE