FastQCFastQC Report
Wed 25 May 2016
SRR2088718_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088718_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences768339
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT131111.7064082390715556No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT121851.5858885205618871No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT73790.9603833724436739No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48230.6277177131448488No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG27220.3542707060295No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG26960.350886783047587No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA26130.34008425968224965No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA17680.23010676277007935No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG16580.21579016553890926No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA15020.19548662764743166No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14560.1894996869871242No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14010.18234138837153913No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA11600.15097502534688464No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC11110.14459763203481796No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC10590.1378297860709921No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10120.13171269452676487No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA9590.12481469767901929No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA7910.10294934918050497No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA7890.10268904741266549No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT7780.10125738768954849No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTC250.005495224629.5999988
ACGTTTA556.2572144E-726.9090926
ATCTATG358.866709E-426.428577
GTATACT659.383439E-825.6153854
GGTATCA33900.025.5943971
CGTTTAT652.680923E-622.7692327
GGGTAAG502.700973E-422.1999991
CGAATTA450.003824669920.55555712
ATTAGAC555.140961E-420.1818183
TCACGTT759.259216E-619.73333424
GTATTGG1601.8189894E-1218.51
TTATGCG609.2330156E-418.54
GACCCTC2700.018.57
TAAGGTG609.2330156E-418.55
CAGCGCG500.00703281318.4999989
TGAGTCG500.00703281318.49999810
TTGCCGT500.00703281318.49999831
CGAACTA852.7212125E-517.41176424
GTATCAA50000.017.392
TGGACCC3000.017.2666665
Produced by FastQC (version 0.11.5)