##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088718_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	768339
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.89740205820608	31.0	31.0	33.0	28.0	34.0
2	31.11497268783701	31.0	31.0	34.0	28.0	34.0
3	31.097164142390273	31.0	31.0	34.0	28.0	34.0
4	34.90781673193734	35.0	35.0	37.0	32.0	37.0
5	34.722116149251825	35.0	35.0	37.0	32.0	37.0
6	34.89033746822692	35.0	35.0	37.0	32.0	37.0
7	34.736100861729	35.0	35.0	37.0	32.0	37.0
8	34.82496398074287	35.0	35.0	37.0	32.0	37.0
9	36.28981868680361	38.0	35.0	39.0	32.0	39.0
10	35.99544732208049	37.0	35.0	39.0	30.0	39.0
11	36.18478301895387	38.0	35.0	39.0	32.0	39.0
12	35.95629663468859	37.0	35.0	39.0	30.0	39.0
13	36.061215166742805	37.0	35.0	39.0	31.0	39.0
14	36.78324021037589	38.0	36.0	40.0	31.0	41.0
15	36.85544792077455	38.0	36.0	40.0	31.0	41.0
16	36.663654975212765	38.0	36.0	40.0	31.0	41.0
17	36.77908319114349	38.0	36.0	40.0	31.0	41.0
18	36.741073927003576	38.0	36.0	40.0	31.0	41.0
19	36.82724292272031	39.0	36.0	40.0	31.0	41.0
20	36.6977102554992	39.0	35.0	40.0	30.0	41.0
21	36.61863578446493	38.0	35.0	40.0	30.0	41.0
22	36.46770501041858	38.0	35.0	40.0	30.0	41.0
23	36.268013207711704	38.0	35.0	40.0	30.0	41.0
24	36.08589828187818	38.0	34.0	40.0	29.0	41.0
25	36.24572486884045	38.0	35.0	40.0	30.0	41.0
26	36.055540588203904	38.0	35.0	40.0	29.0	41.0
27	35.88793227989208	38.0	34.0	40.0	29.0	41.0
28	35.6736505630978	38.0	34.0	40.0	27.0	41.0
29	35.535699736704764	38.0	34.0	40.0	27.0	41.0
30	35.282936308061934	38.0	34.0	40.0	27.0	41.0
31	35.062510168037804	38.0	34.0	40.0	26.0	41.0
32	34.86118887626425	37.0	33.0	40.0	25.0	41.0
33	34.6906079217637	37.0	33.0	40.0	25.0	41.0
34	34.46219702501109	37.0	33.0	40.0	25.0	41.0
35	34.27910206302166	37.0	33.0	40.0	24.0	41.0
36	34.07073830691921	37.0	33.0	40.0	23.0	41.0
37	33.93235798260924	37.0	33.0	40.0	23.0	41.0
38	33.69420659370408	37.0	32.0	40.0	21.0	41.0
39	33.51676278309444	37.0	32.0	40.0	20.0	41.0
40	33.28005086296544	37.0	31.0	40.0	18.0	41.0
41	33.09846174670295	37.0	31.0	40.0	18.0	41.0
42	32.76454273439198	36.0	31.0	40.0	15.0	41.0
43	32.35700908062717	36.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	7.0
15	7.0
16	36.0
17	70.0
18	147.0
19	329.0
20	644.0
21	1275.0
22	2142.0
23	3556.0
24	5346.0
25	7926.0
26	10987.0
27	14690.0
28	19033.0
29	23916.0
30	29391.0
31	34615.0
32	41624.0
33	50345.0
34	58735.0
35	71400.0
36	91446.0
37	114643.0
38	124855.0
39	61172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.79667698763176	24.544504444001934	16.194153882596094	20.464664685770213
2	21.84114043410526	25.841458002262023	31.646187425081894	20.671214138550823
3	22.853584160116824	24.790229312842378	32.552948633350645	19.803237893690152
4	16.954885799106904	18.851574630469102	33.249386013205104	30.944153557218883
5	12.170539306217698	40.900305724426325	33.720011609458844	13.20914335989713
6	32.13672610657535	37.78540461957547	12.920729001131011	17.157140272718163
7	24.647583944066355	34.56638280758884	21.17060308015082	19.61543016819399
8	27.67216033547692	35.3221689905107	18.43626315988125	18.569407514131132
9	24.39261836246761	14.423581257752113	22.17966288318047	39.0041374965998
10	16.70369459314183	29.454186238105834	32.63168991812208	21.210429250630256
11	33.21138195510055	22.743997115856413	22.98946168292902	21.05515924611402
12	19.957336540251113	28.937747530712354	31.278771479776506	19.826144449260028
13	32.503751599228984	21.28448510358058	26.57043310309642	19.641330194094014
14	19.546059747064774	24.232793077014183	28.94542643286362	27.275720743057423
15	22.354065067632906	30.684892996450785	26.09980750684268	20.861234429073626
16	20.500976782383816	28.88685853509974	28.17532365271059	22.436841029805855
17	21.087436665326113	27.850987649982624	28.22126691473425	22.84030876995701
18	21.90087968982441	25.99087121700187	30.66393870413971	21.44431038903401
19	21.941747067375207	25.298468514548915	32.70027943394778	20.0595049841281
20	22.64065731402415	24.88016357363091	32.468871162338495	20.010307950006442
21	21.539971288715005	25.69373674901313	33.1787140832367	19.587577879035166
22	20.127964349069877	26.144969863562828	32.56843658853709	21.158629198830205
23	21.192728730417173	26.298678057472024	33.16075326125578	19.347839950855025
24	21.105397487307034	26.355814295512786	31.230355350958366	21.30843286622181
25	20.89728622391939	26.961015905739522	32.022713932261674	20.118983938079417
26	20.740714710563957	27.58287682910798	31.90909221059975	19.76731624972831
27	19.856079152561566	27.289256434985077	31.831522283783592	21.023142128669768
28	20.277768016461483	27.194897044143275	31.97065357869378	20.55668136070146
29	20.263841871882075	26.51824259864461	31.5215028782868	21.69641265118652
30	20.67746138097897	27.061101935473797	32.75988853878301	19.501548144764225
31	21.299192153463512	27.046785338242625	32.17056533639448	19.48345717189938
32	21.001927534590852	26.924964110893757	31.42115654678469	20.651951807730704
33	20.6248804238754	27.045223527635585	31.671957300098004	20.65793874839101
34	19.73829260261421	27.32556853159868	32.60878336255221	20.3273555032349
35	19.347059045551507	28.896229398741962	31.75590462022623	20.000806935480302
36	21.602964316532155	26.45472896729178	31.51603654116217	20.426270175013894
37	20.578026105664296	27.515328520353645	31.483498820182238	20.42314655379982
38	20.694511146772456	25.96692345436064	32.5559421036808	20.78262329518611
39	19.91165357999529	25.871783158215315	33.35519868183185	20.861364579957545
40	18.898428948680206	26.353862032253993	32.59108284233913	22.15662617672668
41	20.29455748048713	25.55018032404967	32.73398851288298	21.421273682580217
42	19.474737062676763	27.270124255048877	31.608053216093417	21.647085466180943
43	19.645234720611604	27.162229172279424	31.047623509935068	22.144912597173906
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	355.0
1	299.0
2	243.0
3	334.0
4	425.0
5	425.0
6	819.5
7	1214.0
8	1414.5
9	1615.0
10	2772.0
11	3929.0
12	3929.0
13	7907.0
14	11885.0
15	19138.0
16	26391.0
17	23505.5
18	20620.0
19	20620.0
20	23086.0
21	25552.0
22	20172.0
23	14792.0
24	15715.0
25	16638.0
26	16638.0
27	17820.5
28	19003.0
29	20005.5
30	21008.0
31	22721.0
32	24434.0
33	24434.0
34	26648.5
35	28863.0
36	31259.0
37	33655.0
38	35105.5
39	36556.0
40	36556.0
41	37825.5
42	39095.0
43	41324.0
44	43553.0
45	46796.5
46	50040.0
47	50040.0
48	66341.5
49	82643.0
50	74860.0
51	67077.0
52	59603.5
53	52130.0
54	52130.0
55	46071.0
56	40012.0
57	34640.5
58	29269.0
59	26050.0
60	22831.0
61	22831.0
62	20074.0
63	17317.0
64	14862.0
65	12407.0
66	10501.5
67	8596.0
68	8596.0
69	7246.0
70	5896.0
71	4995.0
72	4094.0
73	3360.5
74	2627.0
75	2627.0
76	2125.0
77	1623.0
78	1262.5
79	902.0
80	704.5
81	507.0
82	507.0
83	379.0
84	251.0
85	198.5
86	146.0
87	106.5
88	67.0
89	67.0
90	57.5
91	48.0
92	33.0
93	18.0
94	12.5
95	7.0
96	7.0
97	5.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	768339.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.76735505445001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.84099083278927	51.30488977329105
2	8.37521592155173	10.0112900728213
3	2.272919134242373	4.075390947189512
4	1.0018470428199238	2.395109916738767
5	0.5534976464510383	1.6540545178620822
6	0.3589643027549676	1.2872608160777543
7	0.2611259393507483	1.0924764711772066
8	0.2028591395227132	0.9699483374315371
9	0.1540926942130638	0.8288741489696081
>10	0.8642517833309036	9.66212717122938
>50	0.06924050409941612	2.855959640149175
>100	0.03669499947062957	4.40917157251561
>500	0.004805297549228484	1.9267198589256402
>1k	0.0028394940063622863	3.2611551457294814
>5k	2.18422615874022E-4	0.9632946567526458
>10k+	4.36845231748044E-4	3.302276953139304
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13111	1.7064082390715556	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12185	1.5858885205618871	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7379	0.9603833724436739	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4823	0.6277177131448488	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2722	0.3542707060295	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2696	0.350886783047587	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2613	0.34008425968224965	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1768	0.23010676277007935	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1658	0.21579016553890926	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1502	0.19548662764743166	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1456	0.1894996869871242	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1401	0.18234138837153913	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1160	0.15097502534688464	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1111	0.14459763203481796	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1059	0.1378297860709921	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1012	0.13171269452676487	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	959	0.12481469767901929	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	791	0.10294934918050497	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	789	0.10268904741266549	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	778	0.10125738768954849	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.3015088391972815E-4	0.0
10	0.0	0.0	0.0	1.3015088391972815E-4	0.0
11	0.0	1.3015088391972815E-4	0.0	1.3015088391972815E-4	0.0
12	0.0	1.3015088391972815E-4	0.0	2.603017678394563E-4	0.0
13	0.0	1.3015088391972815E-4	1.3015088391972815E-4	2.603017678394563E-4	0.0
14	0.0	1.3015088391972815E-4	1.3015088391972815E-4	3.9045265175918445E-4	0.0
15	0.0	2.603017678394563E-4	1.3015088391972815E-4	7.809053035183689E-4	0.0
16	0.0	2.603017678394563E-4	1.3015088391972815E-4	0.0013015088391972814	0.0
17	0.0	3.9045265175918445E-4	1.3015088391972815E-4	0.0023427159105551066	0.0
18	0.0	3.9045265175918445E-4	1.3015088391972815E-4	0.0026030176783945628	0.0
19	0.0	3.9045265175918445E-4	1.3015088391972815E-4	0.003644224749752388	0.0
20	0.0	3.9045265175918445E-4	1.3015088391972815E-4	0.006247242428146951	0.0
21	0.0	5.206035356789126E-4	1.3015088391972815E-4	0.009891467177899339	0.0
22	0.0	5.206035356789126E-4	1.3015088391972815E-4	0.015097502534688464	0.0
23	0.0	5.206035356789126E-4	1.3015088391972815E-4	0.01965278347187895	0.0
24	0.0	5.206035356789126E-4	1.3015088391972815E-4	0.02537942236434699	0.0
25	0.0	5.206035356789126E-4	1.3015088391972815E-4	0.02928394888193883	0.0
26	0.0	5.206035356789126E-4	1.3015088391972815E-4	0.03735330368496197	0.0
27	0.0	5.206035356789126E-4	1.3015088391972815E-4	0.0920166749312478	0.0
28	0.0	5.206035356789126E-4	1.3015088391972815E-4	0.20147356830773916	0.0
29	0.0	5.206035356789126E-4	1.3015088391972815E-4	0.34047471233400883	0.0
30	0.0	5.206035356789126E-4	1.3015088391972815E-4	0.553922161962363	0.0
31	0.0	5.206035356789126E-4	1.3015088391972815E-4	0.7945711463299403	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGCTC	25	0.0054952246	29.599998	8
ACGTTTA	55	6.2572144E-7	26.90909	26
ATCTATG	35	8.866709E-4	26.42857	7
GTATACT	65	9.383439E-8	25.615385	4
GGTATCA	3390	0.0	25.594397	1
CGTTTAT	65	2.680923E-6	22.76923	27
GGGTAAG	50	2.700973E-4	22.199999	1
CGAATTA	45	0.0038246699	20.555557	12
ATTAGAC	55	5.140961E-4	20.181818	3
TCACGTT	75	9.259216E-6	19.733334	24
GTATTGG	160	1.8189894E-12	18.5	1
TTATGCG	60	9.2330156E-4	18.5	4
GACCCTC	270	0.0	18.5	7
TAAGGTG	60	9.2330156E-4	18.5	5
CAGCGCG	50	0.007032813	18.499998	9
TGAGTCG	50	0.007032813	18.499998	10
TTGCCGT	50	0.007032813	18.499998	31
CGAACTA	85	2.7212125E-5	17.411764	24
GTATCAA	5000	0.0	17.39	2
TGGACCC	300	0.0	17.266666	5
>>END_MODULE