FastQCFastQC Report
Wed 25 May 2016
SRR2088717_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088717_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3142088
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT446781.421920710050132No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT399071.270079004789172No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT310590.9884828177950458No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166500.5299024088440553No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG92200.29343544802055194No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG90250.28722938377282875No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA80030.25470324192065913No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA73160.2328388001863729No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG69130.2200129340744117No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA63680.20266778015128792No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA50460.16059384714877495No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT47930.15254187661198543No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC47510.15120518585093734No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45100.14353512696016152No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA41350.13160038802223234No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38750.12332563569193479No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA36830.11721504935571506No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA36000.11457349380412005No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG32540.10356170801072409No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129800.023.3031581
GTATTGG10550.018.9383871
ATTGGAC10600.017.8018883
GGACCCT14200.017.3274656
ACGTTTA1851.8189894E-1217.026
TTGGACC15850.016.6908514
CTAATAC6100.015.7704933
TATTGGA12400.015.6653232
TATACCG1902.7284841E-1115.5789485
GTATCAA198200.015.2984352
TGGACCC16350.015.27522855
TAGGTCG1651.6481863E-814.57575721
GACCCTC16450.014.50767
TAATACT6650.013.6315794
AATACTG7600.013.6315795
CTCTAAT6300.013.50793651
TCTAATA6600.013.4545452
CGACGAA3050.013.34426210
ACCCTCG18300.013.243178
GTATTAT2950.013.1694921
Produced by FastQC (version 0.11.5)