##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088717_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3142088
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.495853394303406	31.0	31.0	34.0	30.0	34.0
2	31.700465422992608	31.0	31.0	34.0	30.0	34.0
3	31.768092109450787	31.0	31.0	34.0	30.0	34.0
4	35.49763437561265	37.0	35.0	37.0	33.0	37.0
5	35.385132115968744	37.0	35.0	37.0	33.0	37.0
6	35.43916561216618	37.0	35.0	37.0	33.0	37.0
7	35.289622060235104	37.0	35.0	37.0	32.0	37.0
8	35.33283950035772	37.0	35.0	37.0	32.0	37.0
9	36.909115530818994	39.0	37.0	39.0	33.0	39.0
10	36.732538681284545	39.0	35.0	39.0	32.0	39.0
11	36.8299929855561	39.0	37.0	39.0	32.0	39.0
12	36.67795109494069	39.0	35.0	39.0	32.0	39.0
13	36.72161696298767	39.0	35.0	39.0	32.0	39.0
14	37.65177200638556	39.0	36.0	41.0	32.0	41.0
15	37.675126858318414	39.0	37.0	41.0	32.0	41.0
16	37.55647550291398	39.0	36.0	41.0	32.0	41.0
17	37.62461872487339	39.0	36.0	41.0	32.0	41.0
18	37.59168266452117	39.0	36.0	41.0	32.0	41.0
19	37.65442056365067	39.0	37.0	41.0	32.0	41.0
20	37.53948902767841	39.0	36.0	41.0	32.0	41.0
21	37.47695226868248	39.0	36.0	41.0	32.0	41.0
22	37.346323527539646	39.0	36.0	41.0	31.0	41.0
23	37.16685974422104	39.0	36.0	41.0	31.0	41.0
24	37.00057955092283	39.0	36.0	41.0	31.0	41.0
25	37.176167567553804	39.0	36.0	41.0	31.0	41.0
26	37.035757114377446	39.0	36.0	41.0	31.0	41.0
27	36.91261097715914	39.0	35.0	41.0	31.0	41.0
28	36.736574214344095	39.0	35.0	41.0	30.0	41.0
29	36.611705019082855	39.0	35.0	40.0	30.0	41.0
30	36.39444534971649	38.0	35.0	40.0	30.0	41.0
31	36.208758634385795	38.0	35.0	40.0	30.0	41.0
32	36.02510846290747	38.0	35.0	40.0	30.0	41.0
33	35.87380779914503	38.0	35.0	40.0	29.0	41.0
34	35.68016013555317	38.0	35.0	40.0	28.0	41.0
35	35.53062804097148	38.0	34.0	40.0	27.0	41.0
36	35.319298186428895	38.0	34.0	40.0	26.0	41.0
37	35.218599224464754	38.0	34.0	40.0	25.0	41.0
38	34.99570890439733	38.0	34.0	40.0	24.0	41.0
39	34.87210956535909	38.0	33.0	40.0	24.0	41.0
40	34.67738777526282	38.0	33.0	40.0	23.0	41.0
41	34.51949149737372	38.0	33.0	40.0	23.0	41.0
42	34.22691025840142	38.0	33.0	40.0	21.0	41.0
43	33.854268562815555	38.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	2.0
14	10.0
15	18.0
16	34.0
17	106.0
18	254.0
19	571.0
20	1296.0
21	2561.0
22	4796.0
23	8322.0
24	13594.0
25	20469.0
26	30097.0
27	41653.0
28	56225.0
29	72514.0
30	90611.0
31	111562.0
32	136303.0
33	166785.0
34	202663.0
35	247359.0
36	317653.0
37	437302.0
38	566524.0
39	612803.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.36179508657937	21.56623239069052	14.483362655660823	22.588609867069287
2	19.077727931235536	24.114060459159642	34.477551233447315	22.33066037615751
3	20.087947886882862	24.477926779899224	33.209477264799716	22.224648068418198
4	13.884461542770286	16.673562293608583	36.07543773439828	33.36653842922286
5	12.540641764329962	39.02239529892225	34.58127207131054	13.85569086543725
6	31.89220034575734	37.42119889703916	13.49497531577728	17.19162544142621
7	25.402184789222964	33.72209817166165	21.43822833733492	19.437488701780474
8	27.181765755764957	34.37058414659296	18.988806169655337	19.45884392798674
9	24.423440718401267	14.46993209610934	22.156699621398253	38.94992756409114
10	16.381686318142584	28.28195773001902	33.177969554003575	22.158386397834814
11	32.563823801243	22.802925952423994	23.646314170704322	20.98693607562869
12	20.74365835711794	27.89336263020005	31.091968143476567	20.271010869205448
13	31.62737644521732	21.205612318942055	26.49120584783112	20.6758053880095
14	20.019744832098908	23.78055611427815	28.579148642558707	27.620550411064237
15	22.48835805999068	29.884777256397655	26.04697895157615	21.57988573203551
16	21.044922993881777	28.78334406929405	27.387838914759865	22.783894022064306
17	21.152749381939653	27.205412451847305	27.73776546041995	23.90407270579309
18	22.45032602524181	25.89758784604378	30.139034934731303	21.51305119398311
19	22.300743963886436	25.14385338666517	31.740995159906404	20.814407489541985
20	23.16049073100435	24.842206838255326	31.231174938448575	20.76612749229175
21	21.91300816527099	25.733143056464364	31.989651467431845	20.364197310832797
22	20.54531890895481	26.17686073719132	31.566366059766626	21.711454294087243
23	21.56114023541034	26.225108908471057	32.220835317152165	19.992915538966447
24	21.396727271801428	26.43942499382576	30.179835828913763	21.984011905459045
25	21.320217638716677	26.691423028253823	31.21939932936315	20.76896000366635
26	21.003103668643273	27.43210883972696	30.994389717920058	20.57039777370971
27	20.011565557680118	27.419219321673992	30.876347193331316	21.692867927314577
28	20.52584141500811	27.07919065283977	30.803242939090186	21.591724993061938
29	20.72052087656361	26.896509582163198	30.398862157902645	21.98410738337055
30	20.86593373578334	27.24198049195312	31.882016035196976	20.010069737066562
31	21.558785113593252	27.063564101323706	31.427763958234145	19.949886826848896
32	21.15602745690127	26.909526404098166	30.59564213351122	21.338804005489344
33	20.664061604894577	26.83559467462401	31.158898159440472	21.34144556104094
34	19.914750955415634	27.344746550701316	31.783069092908917	20.95743340097413
35	19.549929855561015	29.215795356463598	30.75999144517913	20.474283342796255
36	21.67046244408177	27.032406476203086	30.541378853806766	20.755752225908378
37	20.815712354332533	27.59006113132414	30.58841763820746	21.00580887613587
38	20.610498496541155	26.177879168247358	31.799141208012	21.41248112719949
39	20.108030074269085	26.056622220637998	32.479739587178976	21.35560811791395
40	19.114136841488843	26.53916758537635	32.15193209101718	22.194763482117626
41	20.474219690855254	25.604438831757736	32.0438829211658	21.877458556221214
42	19.26620132854331	27.455119016399287	31.44501363424576	21.833666020811638
43	19.59722324772572	27.205953493345824	30.760150575031638	22.436672683896823
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1313.0
1	1171.0
2	1029.0
3	1970.5
4	2912.0
5	2912.0
6	4662.5
7	6413.0
8	6926.0
9	7439.0
10	11561.0
11	15683.0
12	15683.0
13	29399.5
14	43116.0
15	67403.0
16	91690.0
17	83883.0
18	76076.0
19	76076.0
20	82577.0
21	89078.0
22	71839.5
23	54601.0
24	60388.0
25	66175.0
26	66175.0
27	71638.0
28	77101.0
29	81281.0
30	85461.0
31	92829.0
32	100197.0
33	100197.0
34	110431.0
35	120665.0
36	130306.5
37	139948.0
38	145498.5
39	151049.0
40	151049.0
41	153143.0
42	155237.0
43	164446.0
44	173655.0
45	187856.5
46	202058.0
47	202058.0
48	263205.0
49	324352.0
50	302161.0
51	279970.0
52	253325.5
53	226681.0
54	226681.0
55	203114.0
56	179547.0
57	154688.5
58	129830.0
59	115612.0
60	101394.0
61	101394.0
62	87937.0
63	74480.0
64	64042.0
65	53604.0
66	45922.5
67	38241.0
68	38241.0
69	32775.0
70	27309.0
71	23011.0
72	18713.0
73	15225.0
74	11737.0
75	11737.0
76	9396.5
77	7056.0
78	5490.0
79	3924.0
80	3023.0
81	2122.0
82	2122.0
83	1600.0
84	1078.0
85	817.0
86	556.0
87	438.0
88	320.0
89	320.0
90	251.0
91	182.0
92	121.0
93	60.0
94	43.0
95	26.0
96	26.0
97	16.5
98	7.0
99	5.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3142088.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.78156217262422
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.47590718493397	34.24710592309961
2	12.622106360154211	11.30475281433443
3	4.419042685982289	5.9367490435738866
4	2.0396687327921597	3.6535820867635898
5	1.0856791540487911	2.4309204268279005
6	0.6693975801149592	1.7986001611273352
7	0.4399461265957791	1.379103237452786
8	0.3123657710149269	1.119058175624372
9	0.23131894349169463	0.9322941284711166
>10	1.449687762256045	12.428678024378021
>50	0.14983254655963682	4.679532042590858
>100	0.09300283675704735	7.980472065362094
>500	0.006556668683309002	1.9973036204259036
>1k	0.004703697098843262	4.233586651984414
>5k	4.988769650288308E-4	1.6561005107960922
>10k+	2.850725514450462E-4	4.222161087187724
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	44678	1.421920710050132	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	39907	1.270079004789172	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	31059	0.9884828177950458	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16650	0.5299024088440553	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	9220	0.29343544802055194	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	9025	0.28722938377282875	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	8003	0.25470324192065913	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7316	0.2328388001863729	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6913	0.2200129340744117	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6368	0.20266778015128792	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	5046	0.16059384714877495	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4793	0.15254187661198543	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4751	0.15120518585093734	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4510	0.14353512696016152	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4135	0.13160038802223234	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3875	0.12332563569193479	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3683	0.11721504935571506	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3600	0.11457349380412005	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	3254	0.10356170801072409	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	6.365194100228893E-5	0.0	0.0	0.0	0.0
6	9.547791150343338E-5	0.0	0.0	0.0	0.0
7	9.547791150343338E-5	0.0	0.0	0.0	0.0
8	1.9095582300686676E-4	0.0	0.0	0.0	0.0
9	1.9095582300686676E-4	0.0	0.0	1.591298525057223E-4	0.0
10	3.182597050114446E-4	0.0	0.0	2.2278179350801125E-4	0.0
11	3.500856755125891E-4	0.0	0.0	4.13737616514878E-4	0.0
12	3.500856755125891E-4	0.0	0.0	5.728674690206003E-4	0.0
13	4.13737616514878E-4	0.0	0.0	6.365194100228892E-4	0.0
14	4.455635870160225E-4	0.0	0.0	7.319973215263227E-4	0.0
15	5.092155280183114E-4	0.0	0.0	0.0010820829970389118	0.0
16	5.410414985194559E-4	0.0	0.0	0.001527646584054934	0.0
17	5.410414985194559E-4	0.0	0.0	0.002482425699089268	0.0
18	5.728674690206003E-4	0.0	0.0	0.003023467197608724	0.0
19	6.046934395217447E-4	0.0	0.0	0.003978246312643058	0.0
20	6.683453805240337E-4	0.0	0.0	0.006747105746242626	0.0
21	7.319973215263227E-4	0.0	0.0	0.011998390878931462	0.0
22	7.63823292027467E-4	0.0	0.0	0.019063756330185533	0.0
23	7.956492625286115E-4	0.0	0.0	0.023137480554332023	0.0
24	8.27475233029756E-4	0.0	0.0	0.03096666929761356	0.0
25	8.27475233029756E-4	0.0	0.0	0.0359315206957921	0.0
26	8.27475233029756E-4	0.0	0.0	0.04640226499066862	0.0
27	9.229531445331894E-4	0.0	0.0	0.113236803043072	0.0
28	9.229531445331894E-4	0.0	0.0	0.2443598015077872	0.0
29	0.0010184310560366228	0.0	0.0	0.4100776299072464	0.0
30	0.0010184310560366228	0.0	0.0	0.6425663444181067	0.0
31	0.0010184310560366228	0.0	0.0	0.900834095034894	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12980	0.0	23.303158	1
GTATTGG	1055	0.0	18.938387	1
ATTGGAC	1060	0.0	17.801888	3
GGACCCT	1420	0.0	17.327465	6
ACGTTTA	185	1.8189894E-12	17.0	26
TTGGACC	1585	0.0	16.690851	4
CTAATAC	610	0.0	15.770493	3
TATTGGA	1240	0.0	15.665323	2
TATACCG	190	2.7284841E-11	15.578948	5
GTATCAA	19820	0.0	15.298435	2
TGGACCC	1635	0.0	15.2752285	5
TAGGTCG	165	1.6481863E-8	14.575757	21
GACCCTC	1645	0.0	14.5076	7
TAATACT	665	0.0	13.631579	4
AATACTG	760	0.0	13.631579	5
CTCTAAT	630	0.0	13.5079365	1
TCTAATA	660	0.0	13.454545	2
CGACGAA	305	0.0	13.344262	10
ACCCTCG	1830	0.0	13.24317	8
GTATTAT	295	0.0	13.169492	1
>>END_MODULE