FastQCFastQC Report
Wed 25 May 2016
SRR2088716_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088716_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2740890
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT393491.4356285732006757No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT351761.2833787565352859No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT270810.9880367325941574No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151290.5519739938487134No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG81630.2978229699112332No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG75220.2744364056930413No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA72160.2632721488275706No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA63890.23309946769115142No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG61010.22259193181776724No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA57500.20978587247208022No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT42960.1567374101113142No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA41740.15228630116495007No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC41180.15024316918956981No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38190.13933430382102166No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA36670.13378865988784666No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35860.13083341542345733No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA32890.11999751905402989No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA32460.11842868557293434No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG29330.1070090372105411No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC27700.10106206378220214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG754.638423E-1027.1333335
GGTATCA115350.022.293021
GTATTGG9750.017.6461541
CGTACCG650.001580688517.0769235
TTGGACC13650.016.8058624
CGTTTAT2050.016.24390227
ATTGGAC10700.015.9065423
TAATACT6350.015.7322854
TCACGTT2250.015.62222224
GGACCCT14450.015.6193776
CGACGAG1902.7284841E-1115.57894824
CTAATAC5000.015.543
TGGACCC15000.015.0466685
ACGTTTA2109.094947E-1214.9761926
GTATACG2251.8189894E-1214.81
ACCGTCC750.004106333514.88
GTATCAA176300.014.61741352
TGCGACG1651.6478225E-814.57575722
CAATGCG1651.6478225E-814.57575719
ATTTCGG1956.7484507E-1014.23076829
Produced by FastQC (version 0.11.5)