##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088716_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2740890
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.468322333256715	31.0	31.0	34.0	30.0	34.0
2	31.672811021237628	31.0	31.0	34.0	30.0	34.0
3	31.739077452944116	31.0	31.0	34.0	30.0	34.0
4	35.471618707792	37.0	35.0	37.0	33.0	37.0
5	35.358197884628716	37.0	35.0	37.0	33.0	37.0
6	35.41179543870786	37.0	35.0	37.0	33.0	37.0
7	35.25991593971301	37.0	35.0	37.0	32.0	37.0
8	35.30674343005374	37.0	35.0	37.0	32.0	37.0
9	36.877081531911166	39.0	37.0	39.0	33.0	39.0
10	36.69797693449938	39.0	35.0	39.0	32.0	39.0
11	36.79929584915848	39.0	37.0	39.0	32.0	39.0
12	36.645861015947375	39.0	35.0	39.0	32.0	39.0
13	36.692893549175636	39.0	35.0	39.0	32.0	39.0
14	37.62385210643258	39.0	36.0	41.0	32.0	41.0
15	37.64396418681523	39.0	36.0	41.0	32.0	41.0
16	37.520642929851256	39.0	36.0	41.0	32.0	41.0
17	37.58921810068992	39.0	36.0	41.0	32.0	41.0
18	37.559255935115964	39.0	36.0	41.0	32.0	41.0
19	37.6228549850596	39.0	37.0	41.0	32.0	41.0
20	37.50978696700707	39.0	36.0	41.0	32.0	41.0
21	37.45120563028797	39.0	36.0	41.0	32.0	41.0
22	37.324940439054465	39.0	36.0	41.0	31.0	41.0
23	37.15145518426497	39.0	36.0	41.0	31.0	41.0
24	36.98917504898044	39.0	36.0	41.0	31.0	41.0
25	37.15917275045697	39.0	36.0	41.0	31.0	41.0
26	37.01158054500545	39.0	36.0	41.0	31.0	41.0
27	36.88744130556133	39.0	35.0	41.0	31.0	41.0
28	36.70066766634196	39.0	35.0	41.0	30.0	41.0
29	36.58596988569406	39.0	35.0	40.0	30.0	41.0
30	36.365809645771996	38.0	35.0	40.0	30.0	41.0
31	36.187763098847455	38.0	35.0	40.0	30.0	41.0
32	36.013023506963066	38.0	35.0	40.0	30.0	41.0
33	35.857419670253094	38.0	35.0	40.0	29.0	41.0
34	35.66547982589597	38.0	34.0	40.0	27.0	41.0
35	35.521642605139206	38.0	34.0	40.0	27.0	41.0
36	35.30079098395047	38.0	34.0	40.0	26.0	41.0
37	35.20914411012481	38.0	34.0	40.0	25.0	41.0
38	34.988094742948455	38.0	34.0	40.0	24.0	41.0
39	34.86990685507262	38.0	33.0	40.0	24.0	41.0
40	34.67047783749074	38.0	33.0	40.0	23.0	41.0
41	34.51142548588232	38.0	33.0	40.0	22.0	41.0
42	34.22702479851435	38.0	33.0	40.0	21.0	41.0
43	33.850953887241005	38.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	5.0
14	6.0
15	17.0
16	35.0
17	88.0
18	214.0
19	537.0
20	1100.0
21	2339.0
22	4281.0
23	7329.0
24	12010.0
25	18578.0
26	26566.0
27	37230.0
28	49157.0
29	64116.0
30	79642.0
31	98207.0
32	119970.0
33	145678.0
34	177536.0
35	215545.0
36	276624.0
37	380050.0
38	492345.0
39	531683.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.17093352889025	21.670807657366765	14.588910901203626	22.56934791253936
2	19.02170462878846	24.07119585244209	34.63061268420112	22.276486834568335
3	20.200080995589023	24.657647698375346	33.00282025181602	22.139451054219613
4	13.895668925057189	16.797135237094523	35.80402715906148	33.503168678786814
5	12.541875084370405	39.00948232143574	34.58993246719132	13.858710127002544
6	32.08384867688962	37.12863340009997	13.541294980827395	17.246222942183014
7	25.311887744491752	33.671362221760084	21.520746910675	19.496003123073162
8	27.044864989109378	34.55147780465469	19.040202270065564	19.363454936170367
9	24.378723699236378	14.598871169583601	22.21676170878802	38.805643422391995
10	16.446738103316804	28.313467523322718	33.202317495412075	22.037476877948404
11	32.47507196567538	22.798871899273593	23.62714300829293	21.0989131267581
12	20.67051213292033	27.84996844090788	31.27965733758013	20.19986208859166
13	31.65303970608087	21.269478162202788	26.571770483310164	20.505711648406173
14	20.0323982356096	23.720871687663497	28.553644983928578	27.693085092798324
15	22.388932062213367	29.98113751372729	26.12505427069309	21.504876153366244
16	20.986176023116577	28.726946356840294	27.50172389260423	22.785153727438896
17	21.091652711345585	27.311968010390785	27.705818183144892	23.89056109511874
18	22.417827785865175	25.928475787061867	30.25010124448628	21.40359518258668
19	22.085089149874676	25.19072272145179	31.84031464232421	20.883873486349323
20	23.114353366972043	24.781220698386292	31.448726508542848	20.655699426098824
21	21.70802184691834	25.823655819824946	32.11788871497944	20.350433618277275
22	20.42664973785887	26.146032857940305	31.604515321665588	21.822802082535233
23	21.445917202076696	26.37880396513541	32.312752427131336	19.86252640565656
24	21.3232198300552	26.434479311464525	30.26246948983724	21.979831368643033
25	21.226900751215847	26.85135120344122	31.14367960771866	20.778068437624274
26	20.98555578662405	27.49471886868864	31.08402745093747	20.43569789374984
27	20.014958644819746	27.423026827052528	30.87858323391307	21.683431294214653
28	20.37991309392205	27.052052435522768	31.022040286184417	21.54599418437077
29	20.599476812276304	26.881669822575876	30.47772803724338	22.04112532790444
30	20.83407214444943	27.24268394572566	32.00569887883133	19.917545030993583
31	21.531619291543986	26.9768213974293	31.532713826530802	19.958845484495914
32	21.07501577954606	26.95649953117418	30.61534756958506	21.353137119694697
33	20.604292766218272	26.78695606171718	31.24262557052636	21.366125601538187
34	19.813856083243035	27.283473616234144	31.935867546672796	20.96680275385003
35	19.535661774095274	29.105910853773775	30.788867849494146	20.56955952263681
36	21.56992801608237	26.853284881917915	30.754134605912675	20.822652496087038
37	20.710791020434968	27.521680913863744	30.69692691060203	21.070601155099254
38	20.50520086541233	26.089408914622624	32.03835250593785	21.367037714027195
39	19.95154858458384	25.874186851716047	32.802994647723914	21.3712699159762
40	19.015210387866716	26.418754492154008	32.31337266362386	22.252662456355417
41	20.326390333066996	25.589607755145227	32.27758136955515	21.806420542232633
42	19.121234343589126	27.41810141961188	31.578903202974217	21.88176103382478
43	19.383156565932964	27.339148962563254	30.869279686525182	22.408414784978604
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	964.0
1	901.0
2	838.0
3	1637.0
4	2436.0
5	2436.0
6	3896.5
7	5357.0
8	6073.5
9	6790.0
10	10572.5
11	14355.0
12	14355.0
13	26910.0
14	39465.0
15	60975.5
16	82486.0
17	74966.5
18	67447.0
19	67447.0
20	72264.5
21	77082.0
22	61169.5
23	45257.0
24	50072.5
25	54888.0
26	54888.0
27	60480.0
28	66072.0
29	70261.5
30	74451.0
31	80773.5
32	87096.0
33	87096.0
34	95194.5
35	103293.0
36	111769.0
37	120245.0
38	126288.5
39	132332.0
40	132332.0
41	135658.0
42	138984.0
43	147504.5
44	156025.0
45	168774.5
46	181524.0
47	181524.0
48	236447.5
49	291371.0
50	269021.0
51	246671.0
52	221866.5
53	197062.0
54	197062.0
55	174387.5
56	151713.0
57	129657.0
58	107601.0
59	95861.0
60	84121.0
61	84121.0
62	73573.5
63	63026.0
64	54662.0
65	46298.0
66	39584.0
67	32870.0
68	32870.0
69	27964.0
70	23058.0
71	19593.0
72	16128.0
73	13143.5
74	10159.0
75	10159.0
76	8176.5
77	6194.0
78	4885.5
79	3577.0
80	2709.0
81	1841.0
82	1841.0
83	1360.0
84	879.0
85	687.5
86	496.0
87	369.0
88	242.0
89	242.0
90	179.5
91	117.0
92	83.5
93	50.0
94	35.5
95	21.0
96	21.0
97	13.5
98	6.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2740890.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.523901420857115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.003055727044	37.07009530897663
2	12.284926483834214	11.676552703604282
3	4.049819159654471	5.773896195471526
4	1.7813479291007899	3.3862641351533567
5	0.9445421429442586	2.244416384456404
6	0.5696578590874284	1.6243418363332478
7	0.3888945172639652	1.293724929109515
8	0.26341862820376927	1.0014944735338676
9	0.20868865723393046	0.8925929256632716
>10	1.285257504887532	11.667226097186619
>50	0.1295969015499616	4.296680524661679
>100	0.08007320138097028	7.302385436332967
>500	0.0052449465568632225	1.704977266111779
>1k	0.004705025587772658	4.278260199017814
>5k	4.627894020759991E-4	1.5080616108418174
>10k+	3.085262680506661E-4	4.279029973545115
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	39349	1.4356285732006757	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	35176	1.2833787565352859	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	27081	0.9880367325941574	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15129	0.5519739938487134	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8163	0.2978229699112332	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7522	0.2744364056930413	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7216	0.2632721488275706	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6389	0.23309946769115142	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6101	0.22259193181776724	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5750	0.20978587247208022	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4296	0.1567374101113142	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4174	0.15228630116495007	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4118	0.15024316918956981	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3819	0.13933430382102166	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3667	0.13378865988784666	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3586	0.13083341542345733	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3289	0.11999751905402989	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3246	0.11842868557293434	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2933	0.1070090372105411	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	2770	0.10106206378220214	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.648449956036178E-5	0.0
8	7.296899912072356E-5	0.0	0.0	3.648449956036178E-5	0.0
9	1.0945349868108534E-4	0.0	0.0	2.1890699736217068E-4	0.0
10	1.0945349868108534E-4	0.0	0.0	2.918759964828942E-4	0.0
11	1.824224978018089E-4	0.0	0.0	5.837519929657885E-4	0.0
12	1.824224978018089E-4	0.0	0.0	8.39143489888321E-4	0.0
13	2.5539149692253247E-4	0.0	0.0	9.85081488129768E-4	0.0
14	3.2836049604325604E-4	0.0	0.0	0.0013499264837333858	0.0
15	4.013294951639796E-4	0.0	0.0	0.00164180248021628	0.0
16	4.013294951639796E-4	0.0	0.0	0.002517430469664963	0.0
17	4.013294951639796E-4	0.0	0.0	0.0035389964573550926	0.0
18	4.013294951639796E-4	0.0	0.0	0.004268686448562329	0.0
19	4.013294951639796E-4	0.0	0.0	0.005582128432735352	0.0
20	4.013294951639796E-4	0.0	0.0	0.008938702392288635	0.0
21	4.013294951639796E-4	0.0	0.0	0.014083016830299647	0.0
22	4.013294951639796E-4	0.0	0.0	0.021817730737096346	0.0
23	4.013294951639796E-4	0.0	0.0	0.026925560675546995	0.0
24	4.013294951639796E-4	0.0	0.0	0.03462379008278333	0.0
25	4.013294951639796E-4	0.0	0.0	0.04108154650496736	0.0
26	4.013294951639796E-4	0.0	0.0	0.05348627635549037	0.0
27	4.013294951639796E-4	0.0	0.0	0.12430269000215259	0.0
28	4.742984942847031E-4	0.0	0.0	0.266336846790641	0.0
29	4.742984942847031E-4	0.0	0.0	0.44554870863113805	0.0
30	5.107829938450649E-4	0.0	0.0	0.7000280930646615	0.0
31	5.107829938450649E-4	0.0	0.0	1.0079937538536752	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTCCG	75	4.638423E-10	27.133333	5
GGTATCA	11535	0.0	22.29302	1
GTATTGG	975	0.0	17.646154	1
CGTACCG	65	0.0015806885	17.076923	5
TTGGACC	1365	0.0	16.805862	4
CGTTTAT	205	0.0	16.243902	27
ATTGGAC	1070	0.0	15.906542	3
TAATACT	635	0.0	15.732285	4
TCACGTT	225	0.0	15.622222	24
GGACCCT	1445	0.0	15.619377	6
CGACGAG	190	2.7284841E-11	15.578948	24
CTAATAC	500	0.0	15.54	3
TGGACCC	1500	0.0	15.046668	5
ACGTTTA	210	9.094947E-12	14.97619	26
GTATACG	225	1.8189894E-12	14.8	1
ACCGTCC	75	0.0041063335	14.8	8
GTATCAA	17630	0.0	14.6174135	2
TGCGACG	165	1.6478225E-8	14.575757	22
CAATGCG	165	1.6478225E-8	14.575757	19
ATTTCGG	195	6.7484507E-10	14.230768	29
>>END_MODULE