FastQCFastQC Report
Wed 25 May 2016
SRR2088715_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088715_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000691
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT204712.0456864306764024No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT203722.035793266852605No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT125601.2551327033020183No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73630.7357915680264937No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG64500.6445546127625811No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG54280.5424251841977193No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA52450.5241378207658508No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG36060.3603509974607546No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA32590.32567495860360496No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA31010.30988586886461456No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22300.22284601340473734No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20130.20116099775055438No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC19900.19886258595310638No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC19690.1967640360510887No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA19450.19436569330592562No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA17550.1753788132400511No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16460.16448633993910208No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG16290.16278751382794487No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG15860.15849048307619434No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT15200.15189504052699584No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA14780.14769794072296044No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA14320.1431011171280645No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG13730.13720519121287192No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA13630.13620588173572062No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT12330.12321485853275385No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT11660.11651948503584024No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA11630.11621969219269485No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC11630.11621969219269485No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA11210.11202259238865943No Hit
TTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCC10780.10772556163690891No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10680.10672625215975762No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT10320.10312873804201297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54550.026.2153991
CGTTTAC400.001931013523.12528
CCGTTTA502.7017642E-422.19999927
CTAGGAC450.003825466820.5555573
TAAACGT555.142455E-420.1818184
CGCCGTT500.007034262618.49999825
GTATCAA79400.018.0107042
ATACTAC752.0669292E-417.2666666
TCCGACG650.00157974117.07692323
TATACCG650.00157974117.07692331
TCAGTAC1005.8762616E-616.653
GGGGTAG904.4450306E-516.4444451
GTACCAT803.3820182E-416.18756
TTTCGTC803.3820182E-416.187517
GATACCT3600.015.93055636
TTGGACC5250.015.8571424
GTTAGCC700.0025921815.8571423
CCGCCGT700.0025921815.85714224
TTTTTAC7750.015.754841
GTATTGG2600.015.6538461
Produced by FastQC (version 0.11.5)