##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088715_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1000691
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.88108417083795	31.0	31.0	31.0	28.0	34.0
2	31.083097579572517	31.0	31.0	34.0	28.0	34.0
3	31.034049471814974	31.0	31.0	34.0	27.0	34.0
4	34.84523294403567	35.0	35.0	37.0	32.0	37.0
5	34.707074411581594	35.0	35.0	37.0	32.0	37.0
6	34.88920855688719	35.0	35.0	37.0	32.0	37.0
7	34.712565617158546	35.0	35.0	37.0	32.0	37.0
8	34.823412022292594	35.0	35.0	37.0	32.0	37.0
9	36.26153427981265	38.0	35.0	39.0	32.0	39.0
10	35.99697908745057	37.0	35.0	39.0	31.0	39.0
11	36.18080606301046	38.0	35.0	39.0	32.0	39.0
12	35.94144446187684	37.0	35.0	39.0	30.0	39.0
13	36.05050709959418	37.0	35.0	39.0	31.0	39.0
14	36.71404059794682	38.0	36.0	40.0	31.0	41.0
15	36.79088250019237	38.0	36.0	40.0	31.0	41.0
16	36.57470088169075	38.0	35.0	40.0	30.0	41.0
17	36.74337632695807	38.0	36.0	40.0	31.0	41.0
18	36.72439943998697	38.0	36.0	40.0	31.0	41.0
19	36.83013537645487	39.0	36.0	40.0	31.0	41.0
20	36.71321516831869	39.0	35.0	40.0	30.0	41.0
21	36.63977691415232	38.0	35.0	40.0	30.0	41.0
22	36.49684567963537	38.0	35.0	40.0	30.0	41.0
23	36.28424058975248	38.0	35.0	40.0	30.0	41.0
24	36.11288799439587	38.0	34.0	40.0	29.0	41.0
25	36.259091967450495	38.0	35.0	40.0	30.0	41.0
26	36.04982357191181	38.0	35.0	40.0	29.0	41.0
27	35.88920955619667	38.0	34.0	40.0	29.0	41.0
28	35.67306691076466	38.0	34.0	40.0	27.0	41.0
29	35.54290685136571	38.0	34.0	40.0	27.0	41.0
30	35.26797383008341	38.0	34.0	40.0	27.0	41.0
31	35.003490588003686	37.0	33.0	40.0	26.0	41.0
32	34.79343773452544	37.0	33.0	40.0	25.0	41.0
33	34.610630054632246	37.0	33.0	40.0	25.0	41.0
34	34.35760589432702	37.0	33.0	40.0	24.0	41.0
35	34.171444531828506	37.0	33.0	40.0	24.0	41.0
36	33.94077892176506	37.0	33.0	40.0	23.0	41.0
37	33.770941279575815	37.0	33.0	40.0	22.0	41.0
38	33.49174620337347	36.0	32.0	40.0	21.0	41.0
39	33.303484292353986	36.0	32.0	40.0	19.0	41.0
40	33.0504361486213	36.0	31.0	40.0	18.0	41.0
41	32.831027759817964	36.0	31.0	40.0	16.0	41.0
42	32.43569693341901	36.0	30.0	39.0	15.0	41.0
43	32.01634270718933	35.0	30.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	4.0
14	3.0
15	12.0
16	23.0
17	78.0
18	213.0
19	452.0
20	847.0
21	1555.0
22	2878.0
23	4651.0
24	7137.0
25	10502.0
26	14544.0
27	19488.0
28	25250.0
29	31864.0
30	38870.0
31	46068.0
32	55401.0
33	65238.0
34	78652.0
35	94023.0
36	121206.0
37	150920.0
38	157515.0
39	73295.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.73374398290781	24.189584996767234	16.286645927663983	19.790025092660972
2	21.97241705981167	26.070585225609104	31.55879287412398	20.398204840455243
3	22.8630016658489	24.6966346254738	33.42050643005683	19.01985727862047
4	16.15953376217034	18.619833694916814	33.47426927992757	31.746363262985277
5	11.541524806358806	41.11798747065777	33.84351413173497	13.496973591248448
6	31.665019471545165	37.44402617791106	12.14151021644044	18.749444134103335
7	24.127827671079284	35.286217223898284	20.670516672978973	19.91543843204346
8	28.27865944632259	34.8133439793103	18.05222591189488	18.85577066247223
9	24.00711108623941	13.987734475477446	22.37963567175082	39.62551876653232
10	16.977968223957244	29.14915793186908	32.133995409172265	21.738878435001414
11	32.503739915718235	22.206355408412787	23.66244924756993	21.627455428299044
12	20.22042768447003	29.327134949749723	31.37811772065503	19.074319645125218
13	33.643252512513854	20.54480354075334	27.162130967501458	18.64981297923135
14	19.252396593953577	24.20237615807477	29.542086418284963	27.00314082968669
15	22.11781658873718	30.08191339784209	27.42834701221456	20.371923001206167
16	19.859177308479843	28.64710485054827	28.961787404903212	22.531930436068677
17	20.882969867821334	27.041114589818438	28.349410557304903	23.72650498505533
18	22.22224442909949	24.753795127566853	31.56199066445086	21.461969778882793
19	22.1707799910262	23.64146374854975	33.86919638529776	20.318559875126287
20	23.145706316934998	23.163194232785145	34.0398784439952	19.651221006284658
21	21.564199138395367	24.35007409879773	35.221162176935735	18.864564585871165
22	19.274381402450906	24.60449829168045	34.72060805983065	21.400512246037987
23	21.036463803511772	24.88550411665539	35.80915587329155	18.26887620654128
24	20.88047159412846	24.87870881221076	32.591079564021264	21.64974002963952
25	20.58587516026426	25.895606136159916	33.661040221207145	19.857478482368684
26	20.3774192033305	26.682462418468837	33.44399020276988	19.496128175430776
27	19.02585313548338	26.68975737765204	33.24902492377767	21.035364563086905
28	19.63663108791825	26.302824748099063	33.4263024250243	20.63424173895838
29	19.899349549461324	25.254449175619648	32.85050030428974	21.995700970629294
30	20.417491513364265	25.872322225342288	34.68233450685576	19.027851754437684
31	21.130998480050287	26.09736671959676	33.78665342248507	18.984981377867893
32	20.978104130046137	25.74021351246289	32.89027282148036	20.391409536010617
33	20.4963370311115	26.005130454855696	33.29739150247179	20.20114101156101
34	19.04144236332694	26.91859924791969	34.17918218511009	19.860776203643283
35	18.555777957431417	29.271973066610972	32.80453206834078	19.367716907616835
36	21.815425540951203	26.015023618679496	32.37672768117231	19.792823159196995
37	20.703393954777248	27.58343984306844	31.983199609070134	19.72996659308418
38	20.672415360985557	25.257347173103383	33.61167433303587	20.45856313287518
39	19.723970736221272	25.38016230784528	34.50645603887714	20.389410917056313
40	18.42087117801599	26.110757466590584	33.19596159054093	22.272409764852487
41	20.70419340235897	25.229266576795435	33.154989901977736	20.91155011886786
42	19.57097645526941	27.571847853133484	31.858185993478504	20.9989896981186
43	20.29267775966807	27.410359441625836	30.60844956135311	21.68851323735299
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	306.0
1	281.5
2	257.0
3	398.0
4	539.0
5	539.0
6	1042.5
7	1546.0
8	1868.5
9	2191.0
10	3740.0
11	5289.0
12	5289.0
13	11142.5
14	16996.0
15	28303.5
16	39611.0
17	35054.5
18	30498.0
19	30498.0
20	34186.5
21	37875.0
22	28533.5
23	19192.0
24	19853.5
25	20515.0
26	20515.0
27	21508.5
28	22502.0
29	23500.5
30	24499.0
31	26164.5
32	27830.0
33	27830.0
34	30742.5
35	33655.0
36	36800.5
37	39946.0
38	41834.5
39	43723.0
40	43723.0
41	45815.5
42	47908.0
43	50135.5
44	52363.0
45	58191.0
46	64019.0
47	64019.0
48	94262.0
49	124505.0
50	110791.5
51	97078.0
52	84633.5
53	72189.0
54	72189.0
55	62108.0
56	52027.0
57	43357.0
58	34687.0
59	30389.5
60	26092.0
61	26092.0
62	22959.0
63	19826.0
64	17147.5
65	14469.0
66	12240.0
67	10011.0
68	10011.0
69	8532.5
70	7054.0
71	5823.0
72	4592.0
73	3822.5
74	3053.0
75	3053.0
76	2394.0
77	1735.0
78	1360.5
79	986.0
80	759.5
81	533.0
82	533.0
83	402.5
84	272.0
85	229.0
86	186.0
87	131.0
88	76.0
89	76.0
90	57.5
91	39.0
92	25.5
93	12.0
94	8.5
95	5.0
96	5.0
97	4.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1000691.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.82813368874151
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.22325140547612	45.0218531863772
2	8.480855032785737	8.960554869336823
3	2.385580692345035	3.7807732722145206
4	1.041419657329407	2.2006502753392514
5	0.5778024489429917	1.5262112509221304
6	0.3857560837353928	1.2227264377691216
7	0.2974190767964845	1.099846632541073
8	0.21397529603348922	0.9043132435956167
9	0.1656653568547876	0.7876612457566009
>10	1.0686143657914453	10.772383104848224
>50	0.09183506161908746	3.4036528242371107
>100	0.05506060662217505	5.613583782093464
>500	0.006668239527990631	2.546614847830134
>1k	0.004763028234279022	4.319743998657084
>5k	7.620845174846437E-4	2.464485462175664
>10k+	5.715633881134827E-4	5.3749455663059305
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	20471	2.0456864306764024	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	20372	2.035793266852605	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12560	1.2551327033020183	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7363	0.7357915680264937	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6450	0.6445546127625811	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5428	0.5424251841977193	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5245	0.5241378207658508	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3606	0.3603509974607546	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3259	0.32567495860360496	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3101	0.30988586886461456	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2230	0.22284601340473734	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2013	0.20116099775055438	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1990	0.19886258595310638	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1969	0.1967640360510887	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1945	0.19436569330592562	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1755	0.1753788132400511	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1646	0.16448633993910208	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1629	0.16278751382794487	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1586	0.15849048307619434	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	1520	0.15189504052699584	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1478	0.14769794072296044	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1432	0.1431011171280645	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1373	0.13720519121287192	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1363	0.13620588173572062	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	1233	0.12321485853275385	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1166	0.11651948503584024	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1163	0.11621969219269485	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	1163	0.11621969219269485	No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA	1121	0.11202259238865943	No Hit
TTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCC	1078	0.10772556163690891	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1068	0.10672625215975762	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	1032	0.10312873804201297	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	9.993094771512884E-5	0.0	0.0	0.0
10	0.0	9.993094771512884E-5	0.0	1.9986189543025768E-4	0.0
11	9.993094771512884E-5	9.993094771512884E-5	0.0	1.9986189543025768E-4	0.0
12	9.993094771512884E-5	9.993094771512884E-5	0.0	3.9972379086051536E-4	0.0
13	9.993094771512884E-5	9.993094771512884E-5	0.0	4.996547385756442E-4	0.0
14	9.993094771512884E-5	9.993094771512884E-5	0.0	5.99585686290773E-4	0.0
15	9.993094771512884E-5	9.993094771512884E-5	0.0	9.993094771512884E-4	0.0
16	9.993094771512884E-5	9.993094771512884E-5	0.0	0.0014989642157269326	0.0
17	9.993094771512884E-5	9.993094771512884E-5	0.0	0.00259820464059335	0.0
18	1.9986189543025768E-4	9.993094771512884E-5	0.0	0.003197790326884123	0.0
19	1.9986189543025768E-4	9.993094771512884E-5	0.0	0.004496892647180798	0.0
20	1.9986189543025768E-4	9.993094771512884E-5	0.0	0.007294959183204406	0.0
21	1.9986189543025768E-4	9.993094771512884E-5	0.0	0.013790470784687781	0.0
22	1.9986189543025768E-4	9.993094771512884E-5	0.0	0.02518259882421247	0.0
23	1.9986189543025768E-4	1.9986189543025768E-4	0.0	0.0311784556871202	0.0
24	1.9986189543025768E-4	1.9986189543025768E-4	0.0	0.038573345818039735	0.0
25	1.9986189543025768E-4	1.9986189543025768E-4	0.0	0.042970307517505406	0.0
26	1.9986189543025768E-4	1.9986189543025768E-4	0.0	0.0512645761778611	0.0
27	1.9986189543025768E-4	1.9986189543025768E-4	0.0	0.09363529800907573	0.0
28	1.9986189543025768E-4	1.9986189543025768E-4	0.0	0.1811748082075286	0.0
29	1.9986189543025768E-4	1.9986189543025768E-4	0.0	0.29809401703422933	0.0
30	1.9986189543025768E-4	1.9986189543025768E-4	0.0	0.4750717254377225	0.0
31	1.9986189543025768E-4	1.9986189543025768E-4	0.0	0.6485518506711863	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5455	0.0	26.215399	1
CGTTTAC	40	0.0019310135	23.125	28
CCGTTTA	50	2.7017642E-4	22.199999	27
CTAGGAC	45	0.0038254668	20.555557	3
TAAACGT	55	5.142455E-4	20.181818	4
CGCCGTT	50	0.0070342626	18.499998	25
GTATCAA	7940	0.0	18.010704	2
ATACTAC	75	2.0669292E-4	17.266666	6
TCCGACG	65	0.001579741	17.076923	23
TATACCG	65	0.001579741	17.076923	31
TCAGTAC	100	5.8762616E-6	16.65	3
GGGGTAG	90	4.4450306E-5	16.444445	1
GTACCAT	80	3.3820182E-4	16.1875	6
TTTCGTC	80	3.3820182E-4	16.1875	17
GATACCT	360	0.0	15.930556	36
TTGGACC	525	0.0	15.857142	4
GTTAGCC	70	0.00259218	15.857142	3
CCGCCGT	70	0.00259218	15.857142	24
TTTTTAC	775	0.0	15.75484	1
GTATTGG	260	0.0	15.653846	1
>>END_MODULE