FastQCFastQC Report
Wed 25 May 2016
SRR2088714_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088714_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818754
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT77960.9521785542421777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT73160.8935528864591806No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT56620.6915386062236032No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32580.3979217200770928No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG17180.20983103593997707No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG15290.186747179250422No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA14160.17294571995984143No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA13260.16195340725052948No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG12770.1559687036643485No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA12630.15425878835401108No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9030.11028953751676329No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8440.10308346585176989No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGCG400.001930715523.1253
GTATTAG1200.023.1249981
GGTATCA23550.021.5244161
CGATAAG609.233724E-418.514
GAGCGTA500.00703319718.4999986
GCGTATC500.00703319718.4999988
TTATACC1451.546141E-1017.8620684
TAGAGCG650.001579409517.0769234
ATTATAC1352.2164386E-816.4444453
CATAACA1151.241051E-616.0869564
CTAATAC1851.8189894E-1116.03
ACGCCTA700.0025916415.8571426
GACCCGA700.0025916415.8571426
GCGATAA700.0025916415.85714213
AGTGTGC855.3618115E-415.2352938
ATTAGAG1353.9677798E-715.0740753
TTAACGG750.00410306614.835
GTATTGG2151.2732926E-1114.6279071
GACCTAT908.270432E-414.3888886
GGCACCG1051.6547125E-414.0952399
Produced by FastQC (version 0.11.5)