##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088714_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	818754
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.639488784176933	31.0	31.0	34.0	30.0	34.0
2	31.85449231393068	31.0	31.0	34.0	30.0	34.0
3	31.940817388373066	31.0	31.0	34.0	30.0	34.0
4	35.64083839590402	37.0	35.0	37.0	33.0	37.0
5	35.51914860873962	37.0	35.0	37.0	33.0	37.0
6	35.568257376452515	37.0	35.0	37.0	33.0	37.0
7	35.439400601401644	37.0	35.0	37.0	33.0	37.0
8	35.46261880857987	37.0	35.0	37.0	33.0	37.0
9	37.08385302545087	39.0	37.0	39.0	33.0	39.0
10	36.8991662941494	39.0	37.0	39.0	33.0	39.0
11	36.98675182044912	39.0	37.0	39.0	33.0	39.0
12	36.853334456991966	39.0	37.0	39.0	32.0	39.0
13	36.88755352645605	39.0	37.0	39.0	33.0	39.0
14	37.90988013493674	40.0	37.0	41.0	33.0	41.0
15	37.91460927213791	40.0	37.0	41.0	33.0	41.0
16	37.806275144915325	40.0	37.0	41.0	32.0	41.0
17	37.810306880943486	40.0	37.0	41.0	32.0	41.0
18	37.767232892908005	40.0	37.0	41.0	32.0	41.0
19	37.817536158601975	40.0	37.0	41.0	32.0	41.0
20	37.696544993978655	40.0	37.0	41.0	32.0	41.0
21	37.62600854469108	39.0	37.0	41.0	32.0	41.0
22	37.48575518409681	39.0	36.0	41.0	32.0	41.0
23	37.316158455409074	39.0	36.0	41.0	31.0	41.0
24	37.15986853194	39.0	36.0	41.0	31.0	41.0
25	37.354195521487526	39.0	36.0	41.0	31.0	41.0
26	37.24926778983675	39.0	36.0	41.0	31.0	41.0
27	37.15471069454317	39.0	36.0	41.0	31.0	41.0
28	36.98595304572558	39.0	36.0	41.0	31.0	41.0
29	36.882268666779034	39.0	36.0	41.0	30.0	41.0
30	36.695758432935904	39.0	35.0	40.0	30.0	41.0
31	36.55315393878992	39.0	35.0	40.0	30.0	41.0
32	36.38539903316503	38.0	35.0	40.0	30.0	41.0
33	36.261223761960245	38.0	35.0	40.0	30.0	41.0
34	36.078785569291874	38.0	35.0	40.0	29.0	41.0
35	35.94561858629088	38.0	35.0	40.0	29.0	41.0
36	35.766153203526336	38.0	35.0	40.0	27.0	41.0
37	35.707979441932494	38.0	35.0	40.0	27.0	41.0
38	35.52841512835357	38.0	34.0	40.0	26.0	41.0
39	35.446441788376	38.0	34.0	40.0	26.0	41.0
40	35.30500370074528	38.0	34.0	40.0	25.0	41.0
41	35.18232826954128	38.0	34.0	40.0	24.0	41.0
42	34.95561181991172	38.0	34.0	40.0	24.0	41.0
43	34.60939793881923	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	2.0
16	5.0
17	32.0
18	55.0
19	130.0
20	259.0
21	501.0
22	1043.0
23	1811.0
24	2843.0
25	4429.0
26	6565.0
27	9131.0
28	12555.0
29	16227.0
30	20993.0
31	26593.0
32	32572.0
33	40359.0
34	50058.0
35	62195.0
36	80922.0
37	112279.0
38	154077.0
39	183115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.40266795643136	21.303712714685975	13.573674143882045	23.719945185000626
2	18.837159879524254	23.28868011637195	34.78944835689352	23.084711647210273
3	19.525522928742944	24.92335915305452	32.192087000490986	23.359030917711546
4	13.812573740097758	16.67594906406564	36.6311492829348	32.880327912901805
5	12.787723785166241	38.56347572042396	35.12972150365067	13.519078990759128
6	32.153491769200514	37.56977065150216	14.340571160568377	15.936166418728948
7	26.108941147157754	32.64228327434125	22.366058669637034	18.882716908863955
8	26.527259714151015	34.700532760755976	19.712025834377602	19.060181690715407
9	24.786443791419646	14.572997505966384	21.776137887570627	38.864420815043346
10	16.045723135398422	27.715406581219753	34.32654985502361	21.91232042835821
11	32.92026664907897	22.779736037930807	23.668159178458975	20.631838134531254
12	20.442892492738967	27.24750047022671	31.521922335646607	20.787684701387718
13	30.646201423138088	21.371742916675824	26.313764573974602	21.66829108621149
14	20.39391563277859	22.93900243540795	27.95528327189852	28.711798659914944
15	22.98211672859003	29.622572836285382	25.311876338924755	22.083434096199834
16	21.866763399018506	28.530547637019176	26.689213121401544	22.913475842560768
17	21.435131919966192	27.345454190147468	27.53293419024518	23.68647969964116
18	22.434088871626887	26.378497082151657	29.37524580032586	21.81216824589559
19	22.53937080002052	25.685614971041364	30.290172628164257	21.48484160077386
20	23.255581041436134	25.400669798254423	30.13518101896296	21.208568141346483
21	22.225723477381486	26.132879961502475	30.36504249139546	21.276354069720576
22	21.29039980262692	26.520420052909664	29.994235142668007	22.19494500179541
23	21.898274695451867	26.598465473145783	30.631789279808096	20.87147055159425
24	22.01992295610159	26.828449082386165	28.913080119303235	22.238547842209012
25	22.13216668254445	26.739533486248618	29.68535115553634	21.442948675670593
26	21.68343605038876	27.336049655940613	29.674358842827026	21.3061554508436
27	21.056385678726457	27.478461173930143	29.331032275872847	22.134120871470554
28	21.31104092315885	27.049638841459096	29.624893435635123	22.014426799746932
29	21.606123450999934	27.10521108904506	29.143796549390906	22.1448689105641
30	21.671833053640043	27.206340365970732	30.14995957271659	20.971867007672635
31	22.06792272159892	26.94423477625758	30.084616380500123	20.903226121643375
32	21.54493291025143	26.888906802287377	29.536466386729103	22.029693900732088
33	21.33473546388781	26.86618935602147	29.98947180716064	21.809603372930088
34	20.947195372480625	27.11889041152776	30.332309826883286	21.601604389108328
35	20.770951958707986	28.435891610911213	29.52144355935971	21.271712871021087
36	22.166975672790606	27.035470971744868	29.2747272074396	21.522826148024926
37	21.381391724498442	27.067104404986114	29.59912256917218	21.95238130134326
38	21.21394216089326	26.32231415052629	30.381286686843666	22.082457001736785
39	20.820783776323534	26.13910893870442	31.093100003175557	21.947007281796484
40	20.232695046375344	26.36897041113692	30.841009631708673	22.557324910779062
41	21.114278525662165	25.6580120524602	30.82696389880233	22.400745523075308
42	20.167718264582525	27.066493720946706	30.34183649789803	22.423951516572743
43	20.42725898133017	26.78789966216959	29.927548445564845	22.8572929109354
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	363.0
1	331.5
2	300.0
3	501.0
4	702.0
5	702.0
6	1102.5
7	1503.0
8	1574.0
9	1645.0
10	2506.5
11	3368.0
12	3368.0
13	6011.0
14	8654.0
15	12781.0
16	16908.0
17	15869.5
18	14831.0
19	14831.0
20	16247.5
21	17664.0
22	14902.0
23	12140.0
24	13907.0
25	15674.0
26	15674.0
27	17687.5
28	19701.0
29	21488.5
30	23276.0
31	25768.0
32	28260.0
33	28260.0
34	31345.5
35	34431.0
36	37022.0
37	39613.0
38	42001.0
39	44389.0
40	44389.0
41	45287.0
42	46185.0
43	48428.5
44	50672.0
45	53006.0
46	55340.0
47	55340.0
48	66105.5
49	76871.0
50	72433.5
51	67996.0
52	62768.5
53	57541.0
54	57541.0
55	52168.0
56	46795.0
57	40957.0
58	35119.0
59	31568.5
60	28018.0
61	28018.0
62	24916.0
63	21814.0
64	18985.5
65	16157.0
66	13767.5
67	11378.0
68	11378.0
69	9651.5
70	7925.0
71	6686.0
72	5447.0
73	4443.5
74	3440.0
75	3440.0
76	2740.0
77	2040.0
78	1614.0
79	1188.0
80	942.0
81	696.0
82	696.0
83	512.5
84	329.0
85	253.5
86	178.0
87	143.5
88	109.0
89	109.0
90	84.5
91	60.0
92	39.5
93	19.0
94	16.0
95	13.0
96	13.0
97	7.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	818754.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.6779724069723
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.75804948253304	54.608987086245044
2	8.489987482843647	10.812503773361161
3	2.276554287898647	4.348990833833536
4	0.9787821585076226	2.493074531275407
5	0.5831373801698407	1.8566503001964618
6	0.3659554304859506	1.398197988279962
7	0.25514711264981627	1.1373075559323558
8	0.20072117526233937	1.0225213967880238
9	0.14740622842509535	0.844787677164217
>10	0.8476771978853379	9.858157712898207
>50	0.060989869503479656	2.6895425758435194
>100	0.03097482815872897	3.7538350963723306
>500	0.0026934633112491364	1.186388793490476
>1k	0.0013467316556245682	1.4440277477150187
>5k	5.771707095533864E-4	2.545026930604208
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7796	0.9521785542421777	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7316	0.8935528864591806	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5662	0.6915386062236032	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3258	0.3979217200770928	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1718	0.20983103593997707	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1529	0.186747179250422	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1416	0.17294571995984143	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1326	0.16195340725052948	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1277	0.1559687036643485	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1263	0.15425878835401108	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	903	0.11028953751676329	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	844	0.10308346585176989	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	1.2213680788124393E-4	0.0	0.0	0.0	0.0
9	1.2213680788124393E-4	0.0	0.0	2.4427361576248786E-4	0.0
10	1.2213680788124393E-4	0.0	0.0	6.106840394062197E-4	0.0
11	2.4427361576248786E-4	0.0	1.2213680788124393E-4	8.549576551687075E-4	0.0
12	2.4427361576248786E-4	0.0	1.2213680788124393E-4	0.0014656416945749273	0.0
13	2.4427361576248786E-4	0.0	1.2213680788124393E-4	0.0014656416945749273	0.0
14	2.4427361576248786E-4	0.0	1.2213680788124393E-4	0.0015877785024561713	0.0
15	2.4427361576248786E-4	0.0	1.2213680788124393E-4	0.002076325733981147	0.0
16	2.4427361576248786E-4	0.0	1.2213680788124393E-4	0.002076325733981147	0.0
17	2.4427361576248786E-4	0.0	1.2213680788124393E-4	0.0030534201970310983	0.0
18	2.4427361576248786E-4	0.0	1.2213680788124393E-4	0.0035419674285560743	0.0
19	2.4427361576248786E-4	0.0	1.2213680788124393E-4	0.005129745931012245	0.0
20	2.4427361576248786E-4	0.0	1.2213680788124393E-4	0.00683966124134966	0.0
21	2.4427361576248786E-4	0.0	1.2213680788124393E-4	0.01025949186202449	0.0
22	2.4427361576248786E-4	0.0	1.2213680788124393E-4	0.017343426719136638	0.0
23	2.4427361576248786E-4	0.0	1.2213680788124393E-4	0.021007530955573957	0.0
24	2.4427361576248786E-4	0.0	1.2213680788124393E-4	0.028091465812686105	0.0
25	2.4427361576248786E-4	0.0	1.2213680788124393E-4	0.03261052770429213	0.0
26	2.4427361576248786E-4	0.0	1.2213680788124393E-4	0.04128224106386045	0.0
27	2.4427361576248786E-4	0.0	1.2213680788124393E-4	0.08952628017695181	0.0
28	2.4427361576248786E-4	0.0	1.2213680788124393E-4	0.19102196752626552	0.0
29	2.4427361576248786E-4	0.0	1.2213680788124393E-4	0.312425954560222	0.0
30	2.4427361576248786E-4	0.0	1.2213680788124393E-4	0.47743278200778255	0.0
31	2.4427361576248786E-4	0.0	1.2213680788124393E-4	0.6916607430314844	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGCG	40	0.0019307155	23.125	3
GTATTAG	120	0.0	23.124998	1
GGTATCA	2355	0.0	21.524416	1
CGATAAG	60	9.233724E-4	18.5	14
GAGCGTA	50	0.007033197	18.499998	6
GCGTATC	50	0.007033197	18.499998	8
TTATACC	145	1.546141E-10	17.862068	4
TAGAGCG	65	0.0015794095	17.076923	4
ATTATAC	135	2.2164386E-8	16.444445	3
CATAACA	115	1.241051E-6	16.086956	4
CTAATAC	185	1.8189894E-11	16.0	3
ACGCCTA	70	0.00259164	15.857142	6
GACCCGA	70	0.00259164	15.857142	6
GCGATAA	70	0.00259164	15.857142	13
AGTGTGC	85	5.3618115E-4	15.235293	8
ATTAGAG	135	3.9677798E-7	15.074075	3
TTAACGG	75	0.004103066	14.8	35
GTATTGG	215	1.2732926E-11	14.627907	1
GACCTAT	90	8.270432E-4	14.388888	6
GGCACCG	105	1.6547125E-4	14.095239	9
>>END_MODULE