FastQCFastQC Report
Wed 25 May 2016
SRR2088713_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088713_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2478681
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT292071.178328312517827No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT250021.008681633497816No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT208300.8403663077257623No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110880.4473346913136463No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG67320.2715960625832852No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG58400.23560918085062174No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA55960.22576523562330125No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA52880.21333927197569996No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA49240.19865404221035302No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG46940.18937491351246893No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC32020.1291816090896731No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA31530.12720475123664562No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30080.12135486575319697No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA29810.12026557673214101No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA28350.11437534721087546No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27090.10929199844594766No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25880.1044103698701043No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG25280.10198972760109105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83650.022.115961
ACGTATA1002.8782597E-718.529
GTATTGG7550.018.3774831
TTAGTAC1950.017.0769233
TTGGACC11450.016.9650654
GGACCCT11800.016.7754236
TGGACCC12150.016.4444455
ATTGGAC8800.016.18753
CGCGATA700.002593628615.85714214
GACCCTC12550.014.8884477
ACCGTTA1252.961904E-614.7999998
ACGTTTA1904.5656634E-1014.60526426
TATACTG3950.014.5189875
GACGTAT1152.211773E-514.47826128
GTATCAA128400.014.3648762
CCCTCGC13250.013.8226429
AGCCGTC2102.044544E-913.21428512
TATTGGA10650.013.2018782
TACTATA4950.013.0808092
ACCCTCG13750.013.050918
Produced by FastQC (version 0.11.5)