##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088713_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2478681
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.58828384935375	31.0	31.0	34.0	30.0	34.0
2	31.79293180526256	31.0	31.0	34.0	30.0	34.0
3	31.87684982456395	31.0	31.0	34.0	30.0	34.0
4	35.57935732754638	37.0	35.0	37.0	33.0	37.0
5	35.46768987215378	37.0	35.0	37.0	33.0	37.0
6	35.51836158021141	37.0	35.0	37.0	33.0	37.0
7	35.37464885558085	37.0	35.0	37.0	33.0	37.0
8	35.407192373685845	37.0	35.0	37.0	33.0	37.0
9	37.01099253998397	39.0	37.0	39.0	33.0	39.0
10	36.828213473214184	39.0	37.0	39.0	32.0	39.0
11	36.91877696242477	39.0	37.0	39.0	33.0	39.0
12	36.773583611606334	39.0	37.0	39.0	32.0	39.0
13	36.808416653857435	39.0	37.0	39.0	32.0	39.0
14	37.78915600676327	40.0	37.0	41.0	32.0	41.0
15	37.80219762042796	40.0	37.0	41.0	32.0	41.0
16	37.6945560965691	39.0	37.0	41.0	32.0	41.0
17	37.728932444312115	39.0	37.0	41.0	32.0	41.0
18	37.68377173182027	39.0	37.0	41.0	32.0	41.0
19	37.732540815054456	40.0	37.0	41.0	32.0	41.0
20	37.61588078498201	39.0	37.0	41.0	32.0	41.0
21	37.549629419840635	39.0	36.0	41.0	32.0	41.0
22	37.40733640190085	39.0	36.0	41.0	32.0	41.0
23	37.224403624346984	39.0	36.0	41.0	31.0	41.0
24	37.06440683573239	39.0	36.0	41.0	31.0	41.0
25	37.25039527071051	39.0	36.0	41.0	31.0	41.0
26	37.13066021807566	39.0	36.0	41.0	31.0	41.0
27	37.02115762375231	39.0	36.0	41.0	31.0	41.0
28	36.853847671402654	39.0	35.0	41.0	30.0	41.0
29	36.732686860471354	39.0	35.0	41.0	30.0	41.0
30	36.52793239630271	39.0	35.0	40.0	30.0	41.0
31	36.361512836867675	38.0	35.0	40.0	30.0	41.0
32	36.18591258818702	38.0	35.0	40.0	30.0	41.0
33	36.047829470593435	38.0	35.0	40.0	29.0	41.0
34	35.85447542463108	38.0	35.0	40.0	28.0	41.0
35	35.71103623257692	38.0	35.0	40.0	27.0	41.0
36	35.51132880753917	38.0	34.0	40.0	27.0	41.0
37	35.4406771181931	38.0	34.0	40.0	26.0	41.0
38	35.246011487561326	38.0	34.0	40.0	25.0	41.0
39	35.15097263423571	38.0	34.0	40.0	25.0	41.0
40	34.98291591374606	38.0	34.0	40.0	24.0	41.0
41	34.84047806071052	38.0	34.0	40.0	23.0	41.0
42	34.58080325786174	38.0	33.0	40.0	23.0	41.0
43	34.219563953570464	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	5.0
14	9.0
15	19.0
16	31.0
17	78.0
18	186.0
19	422.0
20	955.0
21	1865.0
22	3608.0
23	6121.0
24	9911.0
25	15150.0
26	21769.0
27	30528.0
28	41318.0
29	53778.0
30	67281.0
31	83380.0
32	102968.0
33	126418.0
34	155825.0
35	191487.0
36	247604.0
37	340559.0
38	455784.0
39	521619.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.61483466408142	21.15193524297802	13.822754924897557	23.410475168043003
2	18.890853643530573	23.597873223702447	34.61970297912478	22.8915701536422
3	19.85842470249298	24.534702125848384	32.52419331087784	23.082679860780793
4	13.780676093454543	16.445762887600303	36.29063199338681	33.48292902555835
5	12.82081074571516	38.62183153056	34.74690773036143	13.810449993363406
6	32.37568690767388	37.271798993093505	13.889483963446688	16.463030135785928
7	26.073907856638268	33.00077742960873	21.72296475423824	19.20234995951476
8	27.12676621154558	34.36295352245811	19.252941382937134	19.257338883059173
9	24.7569170861438	14.418031202885729	21.799255329749975	39.025796381220495
10	16.200632513824893	27.84174324973645	33.68876430650011	22.268859929938543
11	33.00198775074324	22.64890076617362	23.562370470423584	20.786741012659558
12	20.81845142638363	27.17876967629154	31.305278896316224	20.6975000010086
13	31.572275738588385	20.990478403634835	26.202605337274136	21.234640520502637
14	20.41250971787011	22.953659627842388	28.06936431109933	28.564466343188172
15	22.835007812622923	29.597999903981194	25.38826093394027	22.178731349455617
16	21.65623571568911	28.48510962080236	26.693229181165307	23.16542548234323
17	21.548880231058373	27.035346621852508	27.20862426427604	24.207148882813076
18	22.83230476208919	25.94533140811585	29.47180375368997	21.750560076104993
19	22.600003792339553	25.176696799628512	30.680026998230108	21.543272409801826
20	23.562007374083233	24.918252893373534	30.29760586376383	21.2221338687794
21	22.389286882821953	25.749017320098876	30.83329399789646	21.02840179918271
22	21.277364856550722	26.017022763316454	30.47520031823377	22.23041206189905
23	22.12696994893655	26.213780635749416	31.05062732961603	20.608622085698
24	22.04575740081116	26.179730267831964	29.169949662744017	22.604562668612864
25	21.99895831694357	26.417840779027234	30.0538471872742	21.529353716755
26	21.695450120447124	27.08460669202693	29.97102087763613	21.248922309889817
27	20.801668306651806	27.115631257108113	29.89170449928813	22.19099593695195
28	21.311616944657256	26.770084573206475	29.85164286973596	22.066655612400304
29	21.391619171648145	26.699119410686574	29.41225595387224	22.49700546379304
30	21.536534955486406	27.02300134628054	30.64638007069082	20.79408362754223
31	22.216896809230395	26.661074983025244	30.417992472609424	20.704035735134937
32	21.777227485101953	26.64130640449497	29.587510454148795	21.993955656254276
33	21.476059242798893	26.550048190953174	30.072889573123767	21.901002993124166
34	20.718963029127185	26.9670038217907	30.745989500060716	21.568043649021394
35	20.45761435214939	28.591779256790208	29.757883325849516	21.19272306521089
36	22.215605800020253	26.698958034535302	29.629912037894346	21.455524127550095
37	21.49215651388783	27.172072566013938	29.60013813798549	21.735632782112745
38	21.19171446426547	26.050790722969193	30.767049087801134	21.990445724964207
39	20.792590898143004	25.683135506343895	31.483599543466866	22.04067405204623
40	19.91712527751655	26.180658180701755	31.224308412417734	22.677908129363963
41	21.100415906685853	25.357034648669995	31.133251919065017	22.409297525579127
42	19.943389246135343	26.90338127415347	30.75397761954846	22.399251860162725
43	20.171978564405826	26.804901477842446	30.135826272118116	22.887293685633608
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	921.0
1	813.5
2	706.0
3	1290.5
4	1875.0
5	1875.0
6	3085.5
7	4296.0
8	4686.0
9	5076.0
10	7710.5
11	10345.0
12	10345.0
13	19353.0
14	28361.0
15	44093.5
16	59826.0
17	55259.5
18	50693.0
19	50693.0
20	54087.5
21	57482.0
22	47271.5
23	37061.0
24	41866.0
25	46671.0
26	46671.0
27	51375.5
28	56080.0
29	61293.5
30	66507.0
31	72633.0
32	78759.0
33	78759.0
34	86896.0
35	95033.0
36	103141.5
37	111250.0
38	116448.5
39	121647.0
40	121647.0
41	126952.0
42	132257.0
43	138620.0
44	144983.0
45	154659.0
46	164335.0
47	164335.0
48	206540.5
49	248746.0
50	233473.0
51	218200.0
52	200217.5
53	182235.0
54	182235.0
55	164012.5
56	145790.0
57	126850.0
58	107910.0
59	97153.5
60	86397.0
61	86397.0
62	76201.5
63	66006.0
64	57579.0
65	49152.0
66	41899.0
67	34646.0
68	34646.0
69	29616.0
70	24586.0
71	20686.0
72	16786.0
73	13596.5
74	10407.0
75	10407.0
76	8299.0
77	6191.0
78	4893.0
79	3595.0
80	2744.0
81	1893.0
82	1893.0
83	1424.5
84	956.0
85	718.5
86	481.0
87	386.5
88	292.0
89	292.0
90	223.0
91	154.0
92	103.5
93	53.0
94	39.0
95	25.0
96	25.0
97	19.0
98	13.0
99	8.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2478681.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.385008750615846
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.32799617093335	41.555977735831625
2	11.691497461392045	12.249183936456502
3	3.8078520987271935	5.984230965386248
4	1.6558921499001227	3.4697569905037597
5	0.8787714199361925	2.3017224261574274
6	0.5330015383262134	1.6752774149586238
7	0.3606070496826025	1.3223282417209825
8	0.25815472019735286	1.081874982124089
9	0.19571859249937568	0.9227448162664209
>10	1.122439713156793	11.004348527963888
>50	0.1030616732785428	3.7042310396504385
>100	0.057206127844066965	5.536315478671863
>500	0.003553060097103161	1.3227970954833244
>1k	0.0036303005339965878	3.4352181148120624
>5k	3.0896174757417765E-4	0.9490862801803523
>10k+	3.0896174757417765E-4	3.4849059538324187
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	29207	1.178328312517827	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	25002	1.008681633497816	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	20830	0.8403663077257623	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11088	0.4473346913136463	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6732	0.2715960625832852	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5840	0.23560918085062174	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5596	0.22576523562330125	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5288	0.21333927197569996	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4924	0.19865404221035302	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4694	0.18937491351246893	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3202	0.1291816090896731	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3153	0.12720475123664562	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3008	0.12135486575319697	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2981	0.12026557673214101	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2835	0.11437534721087546	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2709	0.10929199844594766	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2588	0.1044103698701043	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2528	0.10198972760109105	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	4.034403781688729E-5	0.0	0.0	0.0	0.0
3	4.034403781688729E-5	0.0	0.0	0.0	0.0
4	4.034403781688729E-5	0.0	0.0	0.0	0.0
5	4.034403781688729E-5	0.0	0.0	0.0	0.0
6	8.068807563377458E-5	0.0	0.0	0.0	4.034403781688729E-5
7	1.2103211345066186E-4	0.0	0.0	0.0	4.034403781688729E-5
8	1.2103211345066186E-4	0.0	0.0	0.0	4.034403781688729E-5
9	1.2103211345066186E-4	0.0	0.0	4.034403781688729E-5	4.034403781688729E-5
10	1.2103211345066186E-4	0.0	0.0	1.2103211345066186E-4	4.034403781688729E-5
11	1.2103211345066186E-4	0.0	0.0	4.8412845380264743E-4	4.034403781688729E-5
12	1.2103211345066186E-4	0.0	0.0	7.261926807039712E-4	4.034403781688729E-5
13	1.2103211345066186E-4	0.0	0.0	7.665367185208584E-4	4.034403781688729E-5
14	1.2103211345066186E-4	0.0	0.0	8.068807563377457E-4	4.034403781688729E-5
15	1.2103211345066186E-4	0.0	0.0	0.0010892890210559567	4.034403781688729E-5
16	1.2103211345066186E-4	0.0	0.0	0.0013313532479572805	4.034403781688729E-5
17	1.2103211345066186E-4	0.0	0.0	0.002501330344647012	4.034403781688729E-5
18	1.2103211345066186E-4	0.0	0.0	0.0030661468740834337	4.034403781688729E-5
19	1.2103211345066186E-4	0.0	0.0	0.004195779932956278	4.034403781688729E-5
20	1.2103211345066186E-4	0.0	0.0	0.007705711223025472	4.034403781688729E-5
21	1.2103211345066186E-4	0.0	0.0	0.012385619609784397	4.034403781688729E-5
22	1.2103211345066186E-4	0.0	0.0	0.020938555626964502	4.034403781688729E-5
23	1.2103211345066186E-4	0.0	0.0	0.025779840164990978	4.034403781688729E-5
24	1.2103211345066186E-4	0.0	0.0	0.032638326593861816	4.034403781688729E-5
25	1.2103211345066186E-4	0.0	0.0	0.03784270747224028	4.034403781688729E-5
26	1.2103211345066186E-4	0.0	0.0	0.048130437115546536	4.034403781688729E-5
27	1.2103211345066186E-4	0.0	0.0	0.10255454413052749	4.034403781688729E-5
28	1.2103211345066186E-4	0.0	0.0	0.21664748307668474	4.034403781688729E-5
29	1.2103211345066186E-4	0.0	0.0	0.3588602163812124	4.034403781688729E-5
30	1.2103211345066186E-4	0.0	0.0	0.5564653136083264	4.034403781688729E-5
31	1.2103211345066186E-4	0.0	0.0	0.8101082793630967	4.034403781688729E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8365	0.0	22.11596	1
ACGTATA	100	2.8782597E-7	18.5	29
GTATTGG	755	0.0	18.377483	1
TTAGTAC	195	0.0	17.076923	3
TTGGACC	1145	0.0	16.965065	4
GGACCCT	1180	0.0	16.775423	6
TGGACCC	1215	0.0	16.444445	5
ATTGGAC	880	0.0	16.1875	3
CGCGATA	70	0.0025936286	15.857142	14
GACCCTC	1255	0.0	14.888447	7
ACCGTTA	125	2.961904E-6	14.799999	8
ACGTTTA	190	4.5656634E-10	14.605264	26
TATACTG	395	0.0	14.518987	5
GACGTAT	115	2.211773E-5	14.478261	28
GTATCAA	12840	0.0	14.364876	2
CCCTCGC	1325	0.0	13.822642	9
AGCCGTC	210	2.044544E-9	13.214285	12
TATTGGA	1065	0.0	13.201878	2
TACTATA	495	0.0	13.080809	2
ACCCTCG	1375	0.0	13.05091	8
>>END_MODULE