FastQCFastQC Report
Wed 25 May 2016
SRR2088712_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088712_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3677035
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT383211.0421712058764738No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT365100.992919566988076No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT278810.7582467939521924No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159190.43293033653473517No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG49350.13421139586650657No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG48330.13143742172701647No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA44850.12197327466287376No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT43300.11775792180384467No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42450.11544627668760292No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA41780.11362415641950649No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41670.11332500234563991No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG38270.10407842188067287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94900.027.1554261
GTATCAA152650.016.8699652
GTATTGG8750.016.4914281
CTAATAC9500.015.3842113
CGTATAC2006.184564E-1114.83
GGACCCT11150.014.4349786
TTATGCG3950.014.0506324
TTAACGG5450.013.91743135
TAACGGC5650.013.42477936
TGGACCC12150.013.2469145
TGCGCTA2703.6379788E-1213.01851810
AATACTG9250.013.05
AACGGCC5850.012.96581237
TAATACT11050.012.8914034
TAGTACT6400.012.718754
GTATTAG9750.012.712821
TTGGACC13100.012.7099244
GACCCTC11700.012.6495727
ATTGGAC11250.012.4977783
ATCAACG232950.012.26982
Produced by FastQC (version 0.11.5)