##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088712_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3677035
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.54116564025091	31.0	31.0	34.0	30.0	34.0
2	31.749206629798195	31.0	31.0	34.0	30.0	34.0
3	31.822321789158927	31.0	31.0	34.0	30.0	34.0
4	35.546429936076215	37.0	35.0	37.0	33.0	37.0
5	35.424489840319715	37.0	35.0	37.0	33.0	37.0
6	35.479050648144494	37.0	35.0	37.0	33.0	37.0
7	35.35572628490074	37.0	35.0	37.0	33.0	37.0
8	35.38014052082724	37.0	35.0	37.0	33.0	37.0
9	36.97604020630753	39.0	37.0	39.0	33.0	39.0
10	36.776706232059254	39.0	37.0	39.0	32.0	39.0
11	36.873236180781525	39.0	37.0	39.0	33.0	39.0
12	36.738048726759466	39.0	35.0	39.0	32.0	39.0
13	36.774116101696066	39.0	37.0	39.0	32.0	39.0
14	37.770650809687695	40.0	37.0	41.0	32.0	41.0
15	37.77753108142838	40.0	37.0	41.0	32.0	41.0
16	37.65936223071034	39.0	37.0	41.0	32.0	41.0
17	37.68477999257554	39.0	37.0	41.0	32.0	41.0
18	37.64536426767763	39.0	37.0	41.0	32.0	41.0
19	37.69162463778561	40.0	37.0	41.0	32.0	41.0
20	37.56491575413342	39.0	37.0	41.0	32.0	41.0
21	37.49899769787342	39.0	36.0	41.0	32.0	41.0
22	37.35015386037936	39.0	36.0	41.0	31.0	41.0
23	37.1890321957773	39.0	36.0	41.0	31.0	41.0
24	37.03591399048418	39.0	36.0	41.0	31.0	41.0
25	37.21359845636498	39.0	36.0	41.0	31.0	41.0
26	37.10126800533582	39.0	36.0	41.0	31.0	41.0
27	36.97813183720035	39.0	36.0	41.0	31.0	41.0
28	36.82032915106873	39.0	35.0	41.0	30.0	41.0
29	36.71577697791835	39.0	35.0	40.0	30.0	41.0
30	36.53292231376639	39.0	35.0	40.0	30.0	41.0
31	36.399030468842426	39.0	35.0	40.0	30.0	41.0
32	36.218342496060004	38.0	35.0	40.0	30.0	41.0
33	36.09507470013204	38.0	35.0	40.0	30.0	41.0
34	35.92171083495262	38.0	35.0	40.0	29.0	41.0
35	35.78748203375818	38.0	35.0	40.0	28.0	41.0
36	35.59804353235691	38.0	34.0	40.0	27.0	41.0
37	35.560772470210374	38.0	34.0	40.0	27.0	41.0
38	35.37499996600522	38.0	34.0	40.0	26.0	41.0
39	35.293842457305956	38.0	34.0	40.0	25.0	41.0
40	35.14876089022813	38.0	34.0	40.0	25.0	41.0
41	35.030698375185445	38.0	34.0	40.0	24.0	41.0
42	34.8140689441357	38.0	33.0	40.0	23.0	41.0
43	34.45412839420892	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	11.0
15	29.0
16	50.0
17	120.0
18	254.0
19	625.0
20	1385.0
21	2947.0
22	5260.0
23	9137.0
24	14146.0
25	21805.0
26	31962.0
27	44222.0
28	60034.0
29	78354.0
30	99328.0
31	123550.0
32	151976.0
33	187521.0
34	231286.0
35	285172.0
36	368076.0
37	505844.0
38	697604.0
39	756332.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.53358208447839	22.24063681743579	14.145391599481647	23.080389498604173
2	18.791145583330046	23.682586649297598	35.16722032833519	22.35904743903716
3	19.354534291895508	25.548655370427532	32.34905297338753	22.747757364289434
4	13.866416827688614	17.548541147962965	36.94261816925866	31.642423855089767
5	12.501866313483553	38.94787512221124	35.178098658293976	13.37215990601123
6	31.09834418220115	38.55339968208081	14.550174257248027	15.798081878470017
7	25.647811348001852	33.20898495662946	22.628721238715432	18.514482456653255
8	26.210384181820405	35.54007508767254	19.55028983950384	18.699250891003214
9	24.870445889147096	14.626757700157873	21.95176276538026	38.55103364531477
10	16.382982484528974	28.102968832224878	33.872155146741875	21.641893536504277
11	32.873769218949505	23.449056100907388	23.27932695772545	20.397847722417655
12	20.218871998770748	27.9703075983775	31.197636138899952	20.613184263951798
13	30.169117237121757	22.002265412213916	26.452889352426617	21.37572799823771
14	20.29504750430714	23.29284328269924	28.37974074220126	28.032368470792363
15	23.09325312378044	29.940237174788926	25.45216458369311	21.514345117737523
16	21.37904588887514	28.82172728842668	27.17219172512636	22.62703509757182
17	21.198465611559314	28.024019352548997	27.820159449121373	22.95735558677032
18	21.880727270749396	26.695720872931588	29.681251334295162	21.742300522023857
19	22.541069095072526	26.066545463940376	30.787740666052947	20.60464477493415
20	22.72534800457434	25.976880829255094	30.507841236213416	20.78992992995715
21	21.906427325277022	26.448945957816555	30.88542806908283	20.759198647823588
22	21.203442447515457	26.864797316315997	30.483283406331456	21.448476829837084
23	21.52457074789878	27.02294103809183	30.923094286565128	20.529393927444257
24	21.609367329927508	27.107520053521384	29.578641486958922	21.704471129592186
25	21.499523393168683	27.17099510883089	30.33716023916008	20.992321258840345
26	21.25255810727937	27.57354770895572	30.29655687258892	20.87733731117599
27	20.82476778165016	27.55056723691779	30.039229977413868	21.585435004018183
28	20.93999649173859	27.359870112740293	30.342082683466437	21.358050712054684
29	21.316713058211302	27.37654115340213	29.856637209055663	21.4501085793309
30	21.17763360968824	27.5854050886108	30.570636395900504	20.66632490580046
31	21.508715581983854	27.381001268685228	30.45045804568083	20.659825103650086
32	20.938609504668843	27.186904666395616	30.22791461054899	21.646571218386555
33	20.82743297249006	27.233899051817566	30.565224426746006	21.373443548946366
34	20.646934282648928	27.094139707672078	31.01604961606294	21.242876393616054
35	20.485418278585872	28.1394384334117	30.27773736176022	21.097405926242203
36	21.600392707711514	27.031698093708656	30.12002877318274	21.247880425397092
37	20.849515982306396	27.06321805476423	30.37093745368211	21.71632850924726
38	20.743506656858038	26.41666451366386	31.157903038725493	21.681925790752604
39	20.314737281532537	26.29316827280676	31.709053626087325	21.683040819573378
40	19.82931900294667	26.599909981819593	31.58710754724935	21.983663467984396
41	20.31294235708934	25.902554639811697	31.68096033896876	22.103542664130202
42	19.5569256207787	27.130146979835658	31.11749004292861	22.195437356457038
43	19.703674291922706	26.73882625539327	30.926520960502145	22.63097849218188
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1699.0
1	2075.0
2	2451.0
3	3743.0
4	5035.0
5	5035.0
6	7398.0
7	9761.0
8	10344.5
9	10928.0
10	15409.5
11	19891.0
12	19891.0
13	32661.0
14	45431.0
15	64801.5
16	84172.0
17	78965.0
18	73758.0
19	73758.0
20	80906.0
21	88054.0
22	74375.5
23	60697.0
24	67849.0
25	75001.0
26	75001.0
27	83947.0
28	92893.0
29	102138.5
30	111384.0
31	123286.0
32	135188.0
33	135188.0
34	148464.5
35	161741.0
36	175277.5
37	188814.0
38	197017.5
39	205221.0
40	205221.0
41	210161.5
42	215102.0
43	224186.5
44	233271.0
45	237402.5
46	241534.0
47	241534.0
48	273521.5
49	305509.0
50	291087.0
51	276665.0
52	258117.5
53	239570.0
54	239570.0
55	220665.0
56	201760.0
57	179675.0
58	157590.0
59	142018.5
60	126447.0
61	126447.0
62	111226.0
63	96005.0
64	83614.5
65	71224.0
66	60171.5
67	49119.0
68	49119.0
69	41756.0
70	34393.0
71	28519.5
72	22646.0
73	18603.5
74	14561.0
75	14561.0
76	11716.5
77	8872.0
78	6996.0
79	5120.0
80	3960.0
81	2800.0
82	2800.0
83	2119.5
84	1439.0
85	1071.0
86	703.0
87	512.5
88	322.0
89	322.0
90	239.5
91	157.0
92	111.5
93	66.0
94	48.0
95	30.0
96	30.0
97	18.0
98	6.0
99	5.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3677035.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.38369041317913
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.09357357890563	44.35877683179294
2	11.563154056018458	12.808202888768458
3	3.824758398368042	6.354877051212676
4	1.5669668629037778	3.4713763049109327
5	0.7907940048664263	2.1898545173060113
6	0.4625123476861033	1.5369384405911886
7	0.2995113310671475	1.1611629984543468
8	0.20839391234447568	0.9233299140222105
9	0.16048749584797492	0.7999550806707553
>10	0.8745169963797934	9.283934876731227
>50	0.09299186518933703	3.554530402152457
>100	0.055166043347884615	5.900518850444188
>500	0.004618301821899431	1.770640950562354
>1k	0.002358281772091879	2.6578933626129273
>5k	0.0	0.0
>10k+	1.965234810076566E-4	3.2280075297672393
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	38321	1.0421712058764738	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	36510	0.992919566988076	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	27881	0.7582467939521924	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15919	0.43293033653473517	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	4935	0.13421139586650657	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4833	0.13143742172701647	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	4485	0.12197327466287376	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4330	0.11775792180384467	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4245	0.11544627668760292	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4178	0.11362415641950649	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4167	0.11332500234563991	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3827	0.10407842188067287	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.0878329958784728E-4	0.0	0.0	0.0
3	0.0	1.0878329958784728E-4	0.0	0.0	0.0
4	0.0	2.9915407386658E-4	0.0	0.0	0.0
5	0.0	3.263498987635418E-4	0.0	0.0	0.0
6	0.0	3.535457236605036E-4	0.0	0.0	2.719582489696182E-5
7	0.0	3.535457236605036E-4	0.0	0.0	2.719582489696182E-5
8	0.0	3.535457236605036E-4	0.0	0.0	2.719582489696182E-5
9	0.0	3.8074154855746544E-4	0.0	3.263498987635418E-4	2.719582489696182E-5
10	0.0	3.8074154855746544E-4	0.0	5.167206730422745E-4	2.719582489696182E-5
11	0.0	4.6232902324835093E-4	0.0	7.342872722179691E-4	2.719582489696182E-5
12	0.0	5.9830814773316E-4	0.0	0.001033441346084549	2.719582489696182E-5
13	2.719582489696182E-5	6.255039726301219E-4	0.0	0.001033441346084549	2.719582489696182E-5
14	2.719582489696182E-5	7.342872722179691E-4	0.0	0.001060637170981511	2.719582489696182E-5
15	2.719582489696182E-5	7.886789220118927E-4	0.0	0.0015229661942298617	2.719582489696182E-5
16	2.719582489696182E-5	0.0010062455211875872	0.0	0.001958099392581251	2.719582489696182E-5
17	2.719582489696182E-5	0.001060637170981511	0.0	0.0027467783145931437	2.719582489696182E-5
18	2.719582489696182E-5	0.0010878329958784727	0.0	0.00329069481253238	2.719582489696182E-5
19	2.719582489696182E-5	0.0010878329958784727	0.0	0.004024982084750349	2.719582489696182E-5
20	2.719582489696182E-5	0.0010878329958784727	0.0	0.006445410500579951	2.719582489696182E-5
21	2.719582489696182E-5	0.0010878329958784727	0.0	0.01003525938697891	2.719582489696182E-5
22	8.158747469088545E-5	0.0012238121203632817	0.0	0.015963949214516587	2.719582489696182E-5
23	8.158747469088545E-5	0.0013053995950541672	0.0	0.019689777225400357	2.719582489696182E-5
24	1.631749493817709E-4	0.0013053995950541672	0.0	0.02678788752350739	2.719582489696182E-5
25	1.631749493817709E-4	0.001332595419951129	0.0	0.03146556940578482	2.719582489696182E-5
26	1.631749493817709E-4	0.001332595419951129	0.0	0.03973310017446122	2.719582489696182E-5
27	1.631749493817709E-4	0.0014141828946420145	0.0	0.08724420626945352	2.719582489696182E-5
28	1.631749493817709E-4	0.0015773578440237855	0.0	0.19028918680404183	2.719582489696182E-5
29	1.631749493817709E-4	0.0015773578440237855	0.0	0.32425582024647576	2.719582489696182E-5
30	1.631749493817709E-4	0.0016317494938177092	0.0	0.524226720713836	2.719582489696182E-5
31	1.631749493817709E-4	0.001658945318714671	0.0	0.841928347160144	2.719582489696182E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9490	0.0	27.155426	1
GTATCAA	15265	0.0	16.869965	2
GTATTGG	875	0.0	16.491428	1
CTAATAC	950	0.0	15.384211	3
CGTATAC	200	6.184564E-11	14.8	3
GGACCCT	1115	0.0	14.434978	6
TTATGCG	395	0.0	14.050632	4
TTAACGG	545	0.0	13.917431	35
TAACGGC	565	0.0	13.424779	36
TGGACCC	1215	0.0	13.246914	5
TGCGCTA	270	3.6379788E-12	13.018518	10
AATACTG	925	0.0	13.0	5
AACGGCC	585	0.0	12.965812	37
TAATACT	1105	0.0	12.891403	4
TAGTACT	640	0.0	12.71875	4
GTATTAG	975	0.0	12.71282	1
TTGGACC	1310	0.0	12.709924	4
GACCCTC	1170	0.0	12.649572	7
ATTGGAC	1125	0.0	12.497778	3
ATCAACG	23295	0.0	12.2698	2
>>END_MODULE