FastQCFastQC Report
Wed 25 May 2016
SRR2088711_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088711_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3773803
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT712091.8869294449127314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT652581.7292370587441899No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT537921.4254056186822683No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262390.6952933155228294No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG205470.5444640327012301No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG196010.5193964814803528No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA181820.48179515464903705No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA165500.43854965402274576No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG160320.4248234473288617No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA131510.34848135951982656No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA102710.2721657701793125No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC93160.2468597327417462No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA91610.24275247012098936No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA90180.23896318912248465No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT74500.19741359048153811No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA72740.19274986002184002No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG66720.17679778197219093No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG63570.1684507643880722No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62600.16588041294153405No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62310.1651119573544247No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA60970.16156116257260913No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG59910.15875232490938188No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA57200.15157123994018765No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT56190.14889489461956545No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC54160.14351570550980006No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC50410.13357877981442062No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT49540.1312734130530926No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT47140.12491378060804975No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA46740.12385384186720928No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG45590.12080651798729292No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT44190.11709673239435126No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG44180.11707023392583024No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG41200.10917369030656873No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT41090.1088822071528376No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAAAAAAA40180.10647084651742551No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT38750.10268156551892084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA196850.025.3182621
GTATTGG14650.022.9829351
ATTGGAC16150.020.2755413
TTGGACC25300.019.377474
GGACCCT24300.019.1090556
TGGACCC24900.018.5742975
ACCGTTA2050.018.048788
TGCGACG1705.456968E-1217.41176422
TAATACT7450.017.1342284
TCACGTT1950.017.07692324
CCGTTTA1757.2759576E-1216.91428627
GACCCTC27200.016.7316177
TATTGGA19550.016.2762172
TTTTTAC31750.016.2566951
GTATCAA309050.016.1205312
CTAATAC8050.016.0869563
AATACTG7500.016.0333335
ACGTTTA2003.6379788E-1215.72526
GATACTG18400.015.18206636
CGTTTAC1853.0559022E-1015.028
Produced by FastQC (version 0.11.5)