FastQCFastQC Report
Wed 25 May 2016
SRR2088710_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088710_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3090822
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT444141.4369640179861538No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT395911.2809213859614044No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT315411.020472870970894No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167780.542832942175253No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG95920.3103381559986308No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG90010.2912170289974641No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA81430.2634574233003389No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA77350.2502570513604471No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG74880.24226564972036563No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA66310.21453839787603426No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA50430.1631604796393969No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC49000.15853387868987603No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT47970.15520143185210925No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA43280.14002747489179254No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42400.13718033584593353No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA41290.13358905818581596No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40330.13048308831760613No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG35980.11640916235228041No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA35720.11556796217964023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126400.022.4956471
GTATTGG10200.021.5833361
TTAACGG2400.018.535
ATTGGAC11700.016.9188043
TTGGACC16000.016.881254
GGACCCT15300.016.807196
TGGACCC16700.015.5089825
TATTGGA14550.014.8762882
ATACGAC1001.0943133E-414.819
GACCCTC17200.014.7354657
GTATTAG5600.014.5357151
GTATCAA196800.014.4672252
CCGTTTA1854.9076334E-914.027
CGTTTAG1601.7863022E-713.87526
CGTTTAC2151.9826984E-1013.76744228
TATACCG2051.4260877E-913.5365855
TACGTTA2051.4260877E-913.53658519
GAACCGT1102.4584652E-413.4545456
TGTTACG1254.849559E-513.3216
TAACGGC3350.013.25373236
Produced by FastQC (version 0.11.5)