##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088710_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3090822
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.623597864904546	31.0	31.0	34.0	30.0	34.0
2	31.82025364126436	31.0	31.0	34.0	30.0	34.0
3	31.893608884626808	31.0	31.0	34.0	30.0	34.0
4	35.59186617669992	37.0	35.0	37.0	33.0	37.0
5	35.49130231375343	37.0	35.0	37.0	33.0	37.0
6	35.55353106714007	37.0	35.0	37.0	33.0	37.0
7	35.41302863768926	37.0	35.0	37.0	33.0	37.0
8	35.45874560230256	37.0	35.0	37.0	33.0	37.0
9	37.05521540871651	39.0	37.0	39.0	33.0	39.0
10	36.890624241706576	39.0	37.0	39.0	33.0	39.0
11	36.97626036051251	39.0	37.0	39.0	33.0	39.0
12	36.84429320096725	39.0	37.0	39.0	32.0	39.0
13	36.882490159575674	39.0	37.0	39.0	33.0	39.0
14	37.86072669341683	40.0	37.0	41.0	32.0	41.0
15	37.87520989562	40.0	37.0	41.0	33.0	41.0
16	37.755929328832266	40.0	37.0	41.0	32.0	41.0
17	37.81711855292864	40.0	37.0	41.0	32.0	41.0
18	37.781173422474666	40.0	37.0	41.0	32.0	41.0
19	37.838864224468445	40.0	37.0	41.0	32.0	41.0
20	37.728939097754576	40.0	37.0	41.0	32.0	41.0
21	37.674191525749464	40.0	37.0	41.0	32.0	41.0
22	37.542892796802924	39.0	36.0	41.0	32.0	41.0
23	37.36765203560736	39.0	36.0	41.0	31.0	41.0
24	37.212896439846745	39.0	36.0	41.0	31.0	41.0
25	37.375796147432624	39.0	36.0	41.0	31.0	41.0
26	37.26557595358128	39.0	36.0	41.0	31.0	41.0
27	37.1518812794784	39.0	36.0	41.0	31.0	41.0
28	36.98267192352067	39.0	35.0	41.0	31.0	41.0
29	36.84898515669941	39.0	35.0	41.0	30.0	41.0
30	36.6388892663505	39.0	35.0	40.0	30.0	41.0
31	36.45646044967973	39.0	35.0	40.0	30.0	41.0
32	36.280364252616295	38.0	35.0	40.0	30.0	41.0
33	36.125691482718835	38.0	35.0	40.0	30.0	41.0
34	35.9296827834149	38.0	35.0	40.0	29.0	41.0
35	35.76497870145871	38.0	35.0	40.0	28.0	41.0
36	35.543925531784105	38.0	35.0	40.0	27.0	41.0
37	35.446319781598554	38.0	34.0	40.0	26.0	41.0
38	35.23963172256442	38.0	34.0	40.0	25.0	41.0
39	35.120187445281545	38.0	34.0	40.0	25.0	41.0
40	34.92637557258231	38.0	34.0	40.0	24.0	41.0
41	34.75233352163276	38.0	34.0	40.0	23.0	41.0
42	34.46554088200485	38.0	33.0	40.0	21.0	41.0
43	34.08432999376865	38.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	5.0
13	4.0
14	5.0
15	16.0
16	37.0
17	105.0
18	195.0
19	506.0
20	1131.0
21	2192.0
22	4053.0
23	7146.0
24	11638.0
25	17772.0
26	26425.0
27	36658.0
28	50081.0
29	65189.0
30	82047.0
31	101497.0
32	125698.0
33	155320.0
34	192770.0
35	237177.0
36	308111.0
37	433945.0
38	566908.0
39	664188.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.58427758052712	21.386026112147512	14.41072957290973	22.618966734415636
2	18.894488262345746	24.083140342601418	34.75230860916611	22.270062785886733
3	20.0261936792219	24.415187933824722	33.274772859776455	22.28384552717691
4	13.744434328473137	16.537477732460815	36.06791332532252	33.65017461374353
5	12.571930703223932	38.91424352486167	34.573230033952136	13.940595737962264
6	32.024846464791565	37.17606513736476	13.506730571996705	17.292357825846974
7	25.382082824568997	33.820032340911254	21.419544703641943	19.378340130877806
8	27.28833947732998	34.23228513321052	19.05654871099015	19.422826678469352
9	24.420299842566152	14.55467186398958	22.240944318372264	38.784083975072
10	16.47141116505577	28.040501847081455	33.248857423688584	22.239229564174188
11	32.41263974437868	22.731590495991032	23.783673081141522	21.072096678488762
12	20.944007775277903	27.751840772454706	31.036112723411442	20.26803872885595
13	31.66345392908424	21.10733002418127	26.660771794687626	20.568444252046866
14	20.11109018895297	23.7064444345226	28.413994723733687	27.768470652790743
15	22.449270776511877	29.87350937711716	26.0446573759343	21.632562470436667
16	21.090344251464497	28.7707283046387	27.289795400705703	22.8491320431911
17	21.22153912454357	27.22291998698081	27.59411574008468	23.961425148390948
18	22.578653833834494	25.817695098585425	30.212415985132758	21.391235082447324
19	22.25469470580965	25.070482868311405	31.708652261437248	20.966170164441692
20	23.29367398057863	24.623902638197865	31.30963219493067	20.77279118629284
21	21.832282803733115	25.827336546717994	31.992880858231242	20.34749979131765
22	20.575465038103133	26.074617043621405	31.547271243701513	21.802646674573946
23	21.59597026292682	26.22347712032592	32.25653240464834	19.924020212098917
24	21.52993604937457	26.218365211584494	30.107427732816706	22.144271006224233
25	21.446042509080108	26.675881043942358	31.07901393221609	20.799062514761445
26	21.120206857593224	27.373138925502666	30.935039287283452	20.57161492962066
27	19.97588343812746	27.448393987101166	30.78640568754849	21.78931688722288
28	20.511986778921596	26.97178938159493	30.816688893763537	21.699534945719943
29	20.63473729642147	26.889610595498542	30.354222921928216	22.121429186151776
30	20.896641734787703	27.18788076440507	31.90821082546973	20.007266675337497
31	21.56565470285898	26.998675433266623	31.383819579386973	20.051850284487426
32	21.232118834407157	26.83684146159177	30.534692712812323	21.396346991188754
33	20.6703588883475	26.70787900435548	31.18891997015681	21.432842137140216
34	19.89363994432549	27.221334648193913	31.831823379023444	21.053202028457154
35	19.599446360870992	29.143735873499022	30.67527020320161	20.581547562428376
36	21.658186721849397	26.863759867116254	30.608103604801574	20.869949806232775
37	20.81135697882311	27.423093274216377	30.648448859235504	21.117100887725012
38	20.498689345423323	26.07490822829655	31.931343830217333	21.495058596062794
39	20.00710490607353	25.82439234611375	32.707998066533754	21.460504681278962
40	18.975308186624787	26.365542887943725	32.35631168666458	22.302837238766905
41	20.332455249768508	25.477106090224545	32.35071447013125	21.839724189875703
42	19.12190349363373	27.36935352472579	31.621685105127373	21.887057876513108
43	19.359607250110162	27.356250214344275	30.848622146471065	22.435520389074494
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1196.0
1	1095.0
2	994.0
3	1934.0
4	2874.0
5	2874.0
6	4566.5
7	6259.0
8	6927.0
9	7595.0
10	11780.5
11	15966.0
12	15966.0
13	29671.5
14	43377.0
15	67406.5
16	91436.0
17	84010.5
18	76585.0
19	76585.0
20	81880.5
21	87176.0
22	69731.0
23	52286.0
24	58007.5
25	63729.0
26	63729.0
27	68120.0
28	72511.0
29	76514.5
30	80518.0
31	87037.5
32	93557.0
33	93557.0
34	102969.0
35	112381.0
36	122517.0
37	132653.0
38	139399.5
39	146146.0
40	146146.0
41	150742.0
42	155338.0
43	165465.5
44	175593.0
45	191459.5
46	207326.0
47	207326.0
48	270439.5
49	333553.0
50	307297.5
51	281042.0
52	252826.5
53	224611.0
54	224611.0
55	198293.0
56	171975.0
57	147062.0
58	122149.0
59	109236.5
60	96324.0
61	96324.0
62	84311.0
63	72298.0
64	62732.0
65	53166.0
66	45354.0
67	37542.0
68	37542.0
69	32251.5
70	26961.0
71	22815.5
72	18670.0
73	15247.5
74	11825.0
75	11825.0
76	9381.5
77	6938.0
78	5400.5
79	3863.0
80	2973.5
81	2084.0
82	2084.0
83	1574.0
84	1064.0
85	822.0
86	580.0
87	440.5
88	301.0
89	301.0
90	271.5
91	242.0
92	161.5
93	81.0
94	59.0
95	37.0
96	37.0
97	24.0
98	11.0
99	10.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3090822.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.31271934821234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.27818617436178	35.016847618577586
2	12.50398970353672	11.331795523385924
3	4.139460488688957	5.627106341309298
4	1.931852989838256	3.5015004940218315
5	0.9478089072895947	2.14738995058746
6	0.5999377662842686	1.631088697801948
7	0.4010440778479854	1.2720678422051868
8	0.3009806502846109	1.0910601388471215
9	0.22141025090164765	0.9029430503931273
>10	1.425905691088703	12.324155526038062
>50	0.1442557293224893	4.5397038796863765
>100	0.09251114571969281	7.949809340569043
>500	0.006721491155603441	2.0190659439465874
>1k	0.00514837620425484	4.620279971231966
>5k	5.005365754136651E-4	1.7377607922823342
>10k+	2.8602090023638E-4	4.287424889116171
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	44414	1.4369640179861538	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	39591	1.2809213859614044	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	31541	1.020472870970894	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16778	0.542832942175253	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	9592	0.3103381559986308	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	9001	0.2912170289974641	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	8143	0.2634574233003389	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7735	0.2502570513604471	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7488	0.24226564972036563	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6631	0.21453839787603426	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	5043	0.1631604796393969	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4900	0.15853387868987603	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4797	0.15520143185210925	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4328	0.14002747489179254	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4240	0.13718033584593353	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4129	0.13358905818581596	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4033	0.13048308831760613	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	3598	0.11640916235228041	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3572	0.11556796217964023	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.235385279385225E-5	0.0
5	0.0	0.0	0.0	3.235385279385225E-5	0.0
6	0.0	0.0	0.0	3.235385279385225E-5	0.0
7	0.0	0.0	0.0	3.235385279385225E-5	0.0
8	6.47077055877045E-5	0.0	0.0	6.47077055877045E-5	0.0
9	6.47077055877045E-5	0.0	0.0	1.29415411175409E-4	0.0
10	6.47077055877045E-5	0.0	0.0	2.2647696955696575E-4	0.0
11	6.47077055877045E-5	0.0	0.0	6.47077055877045E-4	0.0
12	6.47077055877045E-5	0.0	0.0	9.05907878227863E-4	0.0
13	6.47077055877045E-5	0.0	0.0	0.001164738700578681	0.0
14	6.47077055877045E-5	0.0	0.0	0.0013588618173417946	0.0
15	6.47077055877045E-5	0.0	0.0	0.0017471080508680214	0.0
16	9.706155838155676E-5	0.0	0.0	0.0026530159290958843	0.0
17	9.706155838155676E-5	0.0	0.0	0.00388246233526227	0.0
18	9.706155838155676E-5	0.0	0.0	0.004497185538345463	0.0
19	9.706155838155676E-5	0.0	0.0	0.005500154974954883	0.0
20	9.706155838155676E-5	0.0	0.0	0.008670832548752403	0.0
21	9.706155838155676E-5	0.0	0.0	0.01481806457958433	0.0
22	9.706155838155676E-5	0.0	0.0	0.023359481717161325	0.0
23	1.29415411175409E-4	0.0	3.235385279385225E-5	0.0294743598951994	0.0
24	1.29415411175409E-4	0.0	3.235385279385225E-5	0.03765988465204402	0.0
25	1.29415411175409E-4	0.0	3.235385279385225E-5	0.044324778327577585	0.0
26	1.29415411175409E-4	0.0	3.235385279385225E-5	0.056748657800416845	0.0
27	1.6176926396926125E-4	0.0	3.235385279385225E-5	0.1250799949010328	0.0
28	1.6176926396926125E-4	0.0	3.235385279385225E-5	0.26394273109224664	0.0
29	1.6176926396926125E-4	0.0	3.235385279385225E-5	0.4461919838800164	0.0
30	1.6176926396926125E-4	0.0	3.235385279385225E-5	0.7077081760127242	0.0
31	1.941231167631135E-4	0.0	3.235385279385225E-5	1.020666994087657	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12640	0.0	22.495647	1
GTATTGG	1020	0.0	21.583336	1
TTAACGG	240	0.0	18.5	35
ATTGGAC	1170	0.0	16.918804	3
TTGGACC	1600	0.0	16.88125	4
GGACCCT	1530	0.0	16.80719	6
TGGACCC	1670	0.0	15.508982	5
TATTGGA	1455	0.0	14.876288	2
ATACGAC	100	1.0943133E-4	14.8	19
GACCCTC	1720	0.0	14.735465	7
GTATTAG	560	0.0	14.535715	1
GTATCAA	19680	0.0	14.467225	2
CCGTTTA	185	4.9076334E-9	14.0	27
CGTTTAG	160	1.7863022E-7	13.875	26
CGTTTAC	215	1.9826984E-10	13.767442	28
TATACCG	205	1.4260877E-9	13.536585	5
TACGTTA	205	1.4260877E-9	13.536585	19
GAACCGT	110	2.4584652E-4	13.454545	6
TGTTACG	125	4.849559E-5	13.32	16
TAACGGC	335	0.0	13.253732	36
>>END_MODULE