FastQCFastQC Report
Wed 25 May 2016
SRR2088709_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088709_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3993207
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT445251.1150185803040014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT406271.0174028043124235No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT323180.809324435222116No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180720.4525685745817835No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG94980.23785393544587097No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG85630.21443917132269877No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA81840.20494805303105998No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA78200.19583257266653095No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG71600.1793045038737035No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA66710.16705870744992685No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT49850.12483700444279498No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA49410.12373513318993982No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46330.11602203441995369No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC45180.11314214364544588No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44180.11063789079804778No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA41890.10490315177750616No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA41480.10387640811007294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128700.023.2723391
GTATTGG11550.020.1818181
TTGGACC17750.016.6760584
TGGACCC17600.016.2926145
ATTGGAC14450.016.2595163
GGACCCT17750.015.73802956
TGCGACG2150.015.48837222
GTATCAA199250.015.050692
GACCCTC17550.014.7578347
CAATGCG2450.014.34693819
GTCGTAC800.006302221713.8751
ATGCGAC3200.013.87521
TATTGGA16050.013.4859822
CCCTCGC20750.012.8385549
TATACTG5850.012.6495725
TCTATAC3950.012.645573
TACACAG8300.012.4819285
ACCCTCG21450.012.3333348
GTATTAG7850.012.2547771
CCTCGCA21650.012.13394810
Produced by FastQC (version 0.11.5)