##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088709_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3993207
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.61276262412642	31.0	31.0	34.0	30.0	34.0
2	31.819791961698954	31.0	31.0	34.0	30.0	34.0
3	31.8958073047553	31.0	31.0	34.0	30.0	34.0
4	35.60115616345459	37.0	35.0	37.0	33.0	37.0
5	35.485527296731675	37.0	35.0	37.0	33.0	37.0
6	35.539269314112694	37.0	35.0	37.0	33.0	37.0
7	35.40177681748028	37.0	35.0	37.0	33.0	37.0
8	35.43035735437707	37.0	35.0	37.0	33.0	37.0
9	37.04207896059483	39.0	37.0	39.0	33.0	39.0
10	36.856657568716074	39.0	37.0	39.0	32.0	39.0
11	36.953123892650694	39.0	37.0	39.0	33.0	39.0
12	36.80641399256287	39.0	37.0	39.0	32.0	39.0
13	36.8456473706472	39.0	37.0	39.0	32.0	39.0
14	37.84682061310621	40.0	37.0	41.0	32.0	41.0
15	37.8614096389193	40.0	37.0	41.0	33.0	41.0
16	37.74815204921758	40.0	37.0	41.0	32.0	41.0
17	37.77324992167949	40.0	37.0	41.0	32.0	41.0
18	37.732421584956654	39.0	37.0	41.0	32.0	41.0
19	37.77768996197793	40.0	37.0	41.0	32.0	41.0
20	37.6526516156062	39.0	37.0	41.0	32.0	41.0
21	37.58754429710255	39.0	37.0	41.0	32.0	41.0
22	37.45195653518588	39.0	36.0	41.0	32.0	41.0
23	37.27829536510379	39.0	36.0	41.0	31.0	41.0
24	37.12134632639881	39.0	36.0	41.0	31.0	41.0
25	37.29573197682965	39.0	36.0	41.0	31.0	41.0
26	37.185218296972835	39.0	36.0	41.0	31.0	41.0
27	37.07569780379529	39.0	36.0	41.0	31.0	41.0
28	36.91494004693471	39.0	35.0	41.0	30.0	41.0
29	36.79018493156002	39.0	35.0	41.0	30.0	41.0
30	36.59423941709007	39.0	35.0	40.0	30.0	41.0
31	36.43881346496688	39.0	35.0	40.0	30.0	41.0
32	36.26300940572327	38.0	35.0	40.0	30.0	41.0
33	36.12857510266811	38.0	35.0	40.0	30.0	41.0
34	35.94279935901144	38.0	35.0	40.0	29.0	41.0
35	35.80562640504236	38.0	35.0	40.0	28.0	41.0
36	35.605742702544596	38.0	35.0	40.0	27.0	41.0
37	35.53875093377328	38.0	35.0	40.0	26.0	41.0
38	35.35073789062275	38.0	34.0	40.0	25.0	41.0
39	35.25415186340202	38.0	34.0	40.0	25.0	41.0
40	35.0816711981122	38.0	34.0	40.0	24.0	41.0
41	34.937589260962426	38.0	34.0	40.0	24.0	41.0
42	34.6880324511101	38.0	33.0	40.0	23.0	41.0
43	34.332095982001434	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	4.0
13	11.0
14	6.0
15	25.0
16	56.0
17	123.0
18	301.0
19	662.0
20	1465.0
21	3054.0
22	5342.0
23	9571.0
24	15137.0
25	23535.0
26	33932.0
27	47142.0
28	63724.0
29	83470.0
30	106285.0
31	132117.0
32	162728.0
33	200839.0
34	248206.0
35	307421.0
36	397336.0
37	550122.0
38	743322.0
39	857269.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.48870819869844	21.24382732976277	13.76768096419745	23.49978350734134
2	19.03710476316404	23.412059530096986	34.61270602801207	22.9381296787269
3	20.035825841234878	24.326512499852875	32.342951417244336	23.29471024166791
4	13.98735402397121	16.462557538339485	36.095449096428005	33.4546393412613
5	12.902060924965822	38.43662499840354	34.80295411682891	13.858359959801733
6	32.4483804621198	37.22108570880498	13.780978546817133	16.549555282258094
7	26.193983933214582	32.915949511257494	21.566450224093067	19.32361633143486
8	26.833144387456	34.53973210003889	19.155956603301558	19.471166909203554
9	24.864576266644832	14.387408416343053	21.607995778831402	39.14001953818072
10	16.3208669122337	27.84664055732648	33.61794166944013	22.214550860999694
11	33.29496817971119	22.521221664692064	23.264083229344234	20.91972692625251
12	20.866937276229354	27.330864640876367	30.892463125502882	20.90973495739139
13	31.25843463662164	21.131436461971543	25.989060922712	21.621067978694818
14	20.528612716545876	23.037698771939446	27.831589997713618	28.60209851380106
15	23.026905442167163	29.45632420257703	25.273746139381203	22.24302421587461
16	21.86167659227283	28.234699578559287	26.685894320028993	23.217729509138895
17	21.61673562126882	27.017883120008555	27.2416130693951	24.123768189327524
18	22.630106578496935	26.057226685218172	29.29717893412488	22.01548780216002
19	22.81439454553696	25.15992784746696	30.573020632288785	21.452656974707295
20	23.471159897295582	25.01933909261403	30.118924463470087	21.3905765466203
21	22.42899003232239	25.859741305672358	30.64063545916853	21.070633202836717
22	21.361001320492527	26.10165213073101	30.302386027070472	22.234960521705986
23	22.19942517380141	26.215119827246617	30.82600025493294	20.759454744019028
24	22.163038379928715	26.280055103579652	29.011744194578444	22.54516232191319
25	22.032917401977908	26.391243930004126	29.994638394653723	21.58120027336424
26	21.73448559015348	27.156017707071033	29.783354581918744	21.326142120856744
27	20.882088005956113	27.041999074928995	29.72520583080216	22.350707088312728
28	21.369866375572315	26.825055650758898	29.75109980524426	22.053978168424525
29	21.60050806281768	26.70602851292207	29.401556192804428	22.29190723145582
30	21.678265113729392	26.954300140213117	30.512743266251913	20.854691479805577
31	22.290003999291798	26.75791663192016	30.2168908348603	20.735188533927744
32	21.71392567427634	26.661377684652958	29.594609044810348	22.03008759626035
33	21.456538566620765	26.52254190679321	30.07988316157915	21.941036365006873
34	20.85166133386023	26.794328468321325	30.64649541083144	21.707514786987
35	20.5599659621953	28.225108290153756	29.813480743672944	21.401445003978008
36	22.30638181291378	26.632653904493303	29.476358225356208	21.5846060572367
37	21.42718872324926	26.968799764199552	29.72212559979986	21.88188591275133
38	21.307084756688045	25.995847447928444	30.726381076663444	21.970686718720067
39	20.850659632721268	25.812961862482965	31.264294588284553	22.072083916511215
40	19.969137587908666	26.137212521164066	31.226931135801372	22.666718755125892
41	21.000363867938727	25.3869383680836	31.24280809885388	22.369889665123797
42	19.991901246291516	26.90143536260454	30.648173260239204	22.458490130864742
43	20.231407988616667	26.625391571235852	30.200588148823744	22.94261229132374
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1673.0
1	1454.5
2	1236.0
3	2293.5
4	3351.0
5	3351.0
6	5282.0
7	7213.0
8	7658.5
9	8104.0
10	12363.0
11	16622.0
12	16622.0
13	31787.5
14	46953.0
15	70705.5
16	94458.0
17	87025.5
18	79593.0
19	79593.0
20	85691.0
21	91789.0
22	75599.5
23	59410.0
24	66494.0
25	73578.0
26	73578.0
27	81836.5
28	90095.0
29	97647.5
30	105200.0
31	115940.0
32	126680.0
33	126680.0
34	140364.5
35	154049.0
36	167686.5
37	181324.0
38	191073.5
39	200823.0
40	200823.0
41	206997.5
42	213172.0
43	223843.0
44	234514.0
45	247322.5
46	260131.0
47	260131.0
48	322967.0
49	385803.0
50	365751.0
51	345699.0
52	317039.5
53	288380.0
54	288380.0
55	261837.5
56	235295.0
57	207432.0
58	179569.0
59	161853.0
60	144137.0
61	144137.0
62	127085.5
63	110034.0
64	96304.0
65	82574.0
66	70459.0
67	58344.0
68	58344.0
69	50009.5
70	41675.0
71	34910.0
72	28145.0
73	23358.0
74	18571.0
75	18571.0
76	14972.0
77	11373.0
78	8865.0
79	6357.0
80	5002.5
81	3648.0
82	3648.0
83	2711.5
84	1775.0
85	1360.5
86	946.0
87	705.0
88	464.0
89	464.0
90	368.0
91	272.0
92	190.5
93	109.0
94	76.5
95	44.0
96	44.0
97	29.0
98	14.0
99	12.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3993207.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.427665194091404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.85898204829056	34.46096438454334
2	12.69844654048902	11.537215558528068
3	4.50869887969296	6.144589895030003
4	2.114371475736374	3.842038379827559
5	1.1593837809616026	2.633404911649174
6	0.7416532259629709	2.0214944663498198
7	0.48653413063947365	1.5471476714531835
8	0.3507880505569294	1.2748385691830557
9	0.2683396230851726	1.097103830023971
>10	1.573109322247242	13.587184982065567
>50	0.145384758956712	4.59335872941632
>100	0.08375427735294226	7.163284301552599
>500	0.006022898325999263	1.808863356163955
>1k	0.003978428226195164	3.6839444158990973
>5k	3.315356855162637E-4	1.2022608150877145
>10k+	2.2102379034417578E-4	3.402305733226554
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	44525	1.1150185803040014	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	40627	1.0174028043124235	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	32318	0.809324435222116	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18072	0.4525685745817835	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	9498	0.23785393544587097	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	8563	0.21443917132269877	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	8184	0.20494805303105998	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7820	0.19583257266653095	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7160	0.1793045038737035	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6671	0.16705870744992685	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4985	0.12483700444279498	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4941	0.12373513318993982	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4633	0.11602203441995369	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4518	0.11314214364544588	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4418	0.11063789079804778	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4189	0.10490315177750616	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4148	0.10387640811007294	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.5042528473980938E-5	0.0
5	0.0	0.0	0.0	2.5042528473980938E-5	0.0
6	2.5042528473980938E-5	0.0	0.0	2.5042528473980938E-5	0.0
7	2.5042528473980938E-5	0.0	0.0	5.0085056947961875E-5	0.0
8	5.0085056947961875E-5	0.0	0.0	7.512758542194282E-5	0.0
9	7.512758542194282E-5	0.0	0.0	1.5025517084388563E-4	0.0
10	1.7529769931786657E-4	0.0	0.0	2.504252847398094E-4	0.0
11	2.003402277918475E-4	0.0	0.0	4.00680455583695E-4	0.0
12	2.003402277918475E-4	0.0	0.0	5.258930979535997E-4	0.0
13	2.003402277918475E-4	0.0	0.0	5.759781549015616E-4	0.0
14	2.003402277918475E-4	0.0	0.0	7.512758542194282E-4	0.0
15	2.003402277918475E-4	0.0	0.0	9.265735535372948E-4	0.0
16	2.003402277918475E-4	0.0	0.0	0.0015275942369128372	0.0
17	2.003402277918475E-4	0.0	0.0	0.002328955148080227	0.0
18	2.2538275626582844E-4	0.0	0.0	0.0028798907745078077	0.0
19	2.2538275626582844E-4	0.0	0.0	0.0038315068565190837	0.0
20	2.2538275626582844E-4	0.0	0.0	0.00641088728933912	0.0
21	2.2538275626582844E-4	0.0	0.0	0.010192309088910242	0.0
22	2.2538275626582844E-4	0.0	0.0	0.015651580296238088	0.0
23	2.504252847398094E-4	0.0	0.0	0.019082406697173475	0.0
24	2.754678132137903E-4	0.0	0.0	0.02564354915735648	0.0
25	2.754678132137903E-4	0.0	0.0	0.030601969795204707	0.0
26	2.754678132137903E-4	0.0	0.0	0.04054385359937514	0.0
27	2.754678132137903E-4	0.0	0.0	0.09816671161800528	5.0085056947961875E-5
28	2.754678132137903E-4	0.0	0.0	0.21068279205160162	7.512758542194282E-5
29	2.754678132137903E-4	0.0	0.0	0.36201479161986844	1.0017011389592375E-4
30	2.754678132137903E-4	0.0	0.0	0.5654352504140157	1.0017011389592375E-4
31	2.754678132137903E-4	0.0	0.0	0.8221462098007942	1.0017011389592375E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12870	0.0	23.272339	1
GTATTGG	1155	0.0	20.181818	1
TTGGACC	1775	0.0	16.676058	4
TGGACCC	1760	0.0	16.292614	5
ATTGGAC	1445	0.0	16.259516	3
GGACCCT	1775	0.0	15.7380295	6
TGCGACG	215	0.0	15.488372	22
GTATCAA	19925	0.0	15.05069	2
GACCCTC	1755	0.0	14.757834	7
CAATGCG	245	0.0	14.346938	19
GTCGTAC	80	0.0063022217	13.875	1
ATGCGAC	320	0.0	13.875	21
TATTGGA	1605	0.0	13.485982	2
CCCTCGC	2075	0.0	12.838554	9
TATACTG	585	0.0	12.649572	5
TCTATAC	395	0.0	12.64557	3
TACACAG	830	0.0	12.481928	5
ACCCTCG	2145	0.0	12.333334	8
GTATTAG	785	0.0	12.254777	1
CCTCGCA	2165	0.0	12.133948	10
>>END_MODULE