FastQCFastQC Report
Wed 25 May 2016
SRR2088708_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088708_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4262094
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT343590.8061530318195703No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT317630.7452440044729187No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT246550.5784715212756921No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138350.3246056985134537No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG71680.16818024191864375No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG64310.15088827229056892No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA59340.13922733754816294No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA57700.13537946370962256No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA53590.1257363164679146No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG52840.12397661806614307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101150.022.5328731
GTATTGG12850.016.9883271
TTGGACC18650.014.780164
GTATCAA155050.014.6401172
GTATTAG10100.014.103961
ATTGGAC15200.013.9967113
GGACCCT18750.013.4186666
ATAACGC1805.169568E-813.3611113
GACCCTC17950.013.0891377
TGGACCC19700.012.9593925
TAAACGT2001.4657417E-812.9500014
AGTGCGC2001.4657417E-812.9500018
TAGGACC8300.012.704824
ACCGTTA2653.274181E-1112.5660388
CCCTCGC17500.012.3685719
CTAATAC11050.012.221723
TTAACGG5150.012.21359235
GGGTAAG5350.012.1028041
TAATACT13950.012.06814
TACACAG9200.012.0652185
Produced by FastQC (version 0.11.5)