FastQCFastQC Report
Wed 25 May 2016
SRR2088707_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088707_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1664417
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT184131.1062732476296504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT168391.0117056002191758No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT134450.8077903554217484No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69810.41942614140566936No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG44640.26820201908536145No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG40430.2429078770524454No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA37200.2235016825711345No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA34330.20625840759857655No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG33250.1997696490723178No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA31220.18757318628685No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA21020.12629046687218407No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC20330.12214487114707431No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19620.11787911322703386No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA19300.11595651810814236No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA19280.11583635591321166No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG17020.10225802788604059No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16740.10057575715701055No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16700.1003354327671491No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA16660.10009510837728766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50800.024.0354331
ACGTTTA1103.8546204E-818.526
TATACCG902.153396E-618.55
CGTTTAG701.2196139E-418.526
GTATTGG4450.017.4606721
TTGGACC7350.015.6054434
GTATCAA78500.015.5070082
GTAAGAC855.365945E-415.2352943
GGACCCT7000.014.5357156
TACCGAC2600.014.2307697
TAATTCG1051.6563345E-414.0952385
ATTGGAC5700.013.956143
TACACTA1601.7846105E-713.8755
CTTACTC1601.7846105E-713.8753
TTATGCG1907.1304385E-913.6315794
ATACCGA3550.013.5492956
TGGACCC7400.013.55
GACCCTC7450.013.4093957
CGTTTAT1551.888764E-613.12903227
ACAGTCC2305.7661964E-1012.8695658
Produced by FastQC (version 0.11.5)