##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088707_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1664417
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.579988668705017	31.0	31.0	34.0	30.0	34.0
2	31.78960080316411	31.0	31.0	34.0	30.0	34.0
3	31.868701172843103	31.0	31.0	34.0	30.0	34.0
4	35.57917997713314	37.0	35.0	37.0	33.0	37.0
5	35.46401292464569	37.0	35.0	37.0	33.0	37.0
6	35.5110317907111	37.0	35.0	37.0	33.0	37.0
7	35.38148072267947	37.0	35.0	37.0	33.0	37.0
8	35.40744897462595	37.0	35.0	37.0	33.0	37.0
9	37.00757262152453	39.0	37.0	39.0	33.0	39.0
10	36.823343549122605	39.0	37.0	39.0	32.0	39.0
11	36.913518667497385	39.0	37.0	39.0	33.0	39.0
12	36.769624439067854	39.0	37.0	39.0	32.0	39.0
13	36.80229593905854	39.0	37.0	39.0	32.0	39.0
14	37.79692288651221	40.0	37.0	41.0	32.0	41.0
15	37.80474003810343	40.0	37.0	41.0	32.0	41.0
16	37.70395820278212	39.0	37.0	41.0	32.0	41.0
17	37.733080712345526	39.0	37.0	41.0	32.0	41.0
18	37.687093438723586	39.0	37.0	41.0	32.0	41.0
19	37.739282884036875	40.0	37.0	41.0	32.0	41.0
20	37.61801760015669	39.0	37.0	41.0	32.0	41.0
21	37.547814640201345	39.0	36.0	41.0	32.0	41.0
22	37.41792050910319	39.0	36.0	41.0	32.0	41.0
23	37.247258349319914	39.0	36.0	41.0	31.0	41.0
24	37.0879701420978	39.0	36.0	41.0	31.0	41.0
25	37.269717264363436	39.0	36.0	41.0	31.0	41.0
26	37.164051436629165	39.0	36.0	41.0	31.0	41.0
27	37.05938115267989	39.0	36.0	41.0	31.0	41.0
28	36.89920795089211	39.0	36.0	41.0	30.0	41.0
29	36.786384061205815	39.0	35.0	41.0	30.0	41.0
30	36.58561946915947	39.0	35.0	40.0	30.0	41.0
31	36.434714377466705	39.0	35.0	40.0	30.0	41.0
32	36.26617488285688	38.0	35.0	40.0	30.0	41.0
33	36.12806225843644	38.0	35.0	40.0	30.0	41.0
34	35.951811955777906	38.0	35.0	40.0	29.0	41.0
35	35.81764726027191	38.0	35.0	40.0	28.0	41.0
36	35.62694024394127	38.0	35.0	40.0	27.0	41.0
37	35.56731095632885	38.0	35.0	40.0	27.0	41.0
38	35.3900206498732	38.0	34.0	40.0	26.0	41.0
39	35.29984192663257	38.0	34.0	40.0	25.0	41.0
40	35.13628015094775	38.0	34.0	40.0	24.0	41.0
41	35.01807239411758	38.0	34.0	40.0	24.0	41.0
42	34.75665052688118	38.0	33.0	40.0	23.0	41.0
43	34.40715698049227	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	6.0
15	14.0
16	26.0
17	55.0
18	116.0
19	265.0
20	567.0
21	1157.0
22	2162.0
23	3917.0
24	6209.0
25	9804.0
26	14119.0
27	19713.0
28	27204.0
29	35110.0
30	44227.0
31	55223.0
32	68414.0
33	84135.0
34	103931.0
35	128713.0
36	166337.0
37	229093.0
38	310629.0
39	353269.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.338438624455286	21.463130934134895	13.731474744610276	23.46695569679954
2	18.858134710231873	23.548846232644824	34.84409255613227	22.748926500991036
3	19.664663362606845	24.67506640463297	32.60342810725918	23.056842125501
4	13.652287858150933	16.591515227253748	36.6800507324787	33.076146182116624
5	12.628686200633615	38.55944754229259	35.17754264706501	13.634323610008789
6	32.06023490507487	37.63077401877054	13.996192060042645	16.31279901611195
7	25.947103400169546	33.046646363261125	21.903585459653442	19.10266477691588
8	26.75615545863807	34.60575084248719	19.410820725815707	19.227272973059033
9	24.65776304856295	14.367072674696304	21.777655479366047	39.197508797374695
10	16.003561607457748	27.89661485072551	34.062377397010486	22.03744614480626
11	33.022133275495264	22.687223213894114	23.600395814270104	20.69024769634052
12	20.802959835185533	27.2568112438169	31.34815373791544	20.592075183082123
13	31.07286214932917	21.223347274150647	26.322430016035646	21.38136056048454
14	20.403180212651037	23.014184546300598	27.971415817069882	28.611219423978483
15	22.678571535859103	29.785864960523718	25.415926417478314	22.119637086138873
16	21.654909797244322	28.608275450202687	26.77514108543712	22.96167366711587
17	21.436815413445068	27.253146296871517	27.304095067522145	24.005943222161275
18	22.604791948171645	26.097846873710136	29.54475951639523	21.752601661722995
19	22.478261156909596	25.29672552010704	30.706427535887943	21.51858578709542
20	23.448751124267535	25.08896508507183	30.283156204244488	21.179127586416143
21	22.26863820785296	25.87915167893623	30.837524490557357	21.01468562265346
22	21.166871042533213	26.199804496108847	30.489775098427856	22.14354936293008
23	22.008787461315283	26.279051463665652	31.07196093286718	20.640200142151876
24	21.982592102820387	26.314258986780352	29.242010866267286	22.46113804413197
25	22.016958490570573	26.495763982223203	30.089634989308568	21.397642537897653
26	21.6098489741453	27.215114962175946	29.958778359029015	21.216257704649735
27	20.77490196266921	27.257351973694092	29.803648965373462	22.164097098263234
28	21.240530468025742	26.84789929446767	29.880492689031655	22.031077548474933
29	21.459526068286973	26.79815214576636	29.405852019055324	22.33646976689135
30	21.533966548046553	26.99798187593614	30.623575702483212	20.844475873534098
31	22.10828175871792	26.88172495234067	30.34594095109579	20.66405233784562
32	21.713909434955305	26.732483506236722	29.621663321150887	21.93194373765709
33	21.366941097092855	26.690306575816038	30.217848051299644	21.724904275791463
34	20.66615517625691	27.024177234431036	30.78921928819521	21.520448301116847
35	20.475397691804396	28.628703023340908	29.788808934299517	21.10709035055518
36	22.256381663970025	26.852525538972504	29.589700177299317	21.30139261975815
37	21.34825587578113	27.372647599730115	29.673092740581236	21.606003783907518
38	21.242573225339566	26.291127764256196	30.631506407348642	21.834792603055604
39	20.846999279627642	26.039748452461133	31.428962814006344	21.68428945390488
40	19.990543235258954	26.466083920075317	31.05195392741122	22.49141891725451
41	21.149687848658118	25.65048302198307	31.007493915286855	22.192335214071953
42	20.090998830221032	27.201656796343705	30.502151804505722	22.20519256892954
43	20.356977848700176	26.986205980832928	29.892388746329797	22.7644274241371
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	571.0
1	515.0
2	459.0
3	829.5
4	1200.0
5	1200.0
6	1941.0
7	2682.0
8	2869.0
9	3056.0
10	4743.5
11	6431.0
12	6431.0
13	12034.0
14	17637.0
15	27525.5
16	37414.0
17	35228.0
18	33042.0
19	33042.0
20	36097.0
21	39152.0
22	32420.5
23	25689.0
24	28846.5
25	32004.0
26	32004.0
27	35801.5
28	39599.0
29	43225.0
30	46851.0
31	51458.5
32	56066.0
33	56066.0
34	61746.5
35	67427.0
36	73181.5
37	78936.0
38	82933.5
39	86931.0
40	86931.0
41	89600.0
42	92269.0
43	96112.5
44	99956.0
45	104676.0
46	109396.0
47	109396.0
48	136285.0
49	163174.0
50	153014.5
51	142855.0
52	130441.5
53	118028.0
54	118028.0
55	106826.5
56	95625.0
57	83106.5
58	70588.0
59	63392.0
60	56196.0
61	56196.0
62	49630.0
63	43064.0
64	37556.0
65	32048.0
66	27544.5
67	23041.0
68	23041.0
69	19671.0
70	16301.0
71	13609.0
72	10917.0
73	8877.0
74	6837.0
75	6837.0
76	5489.5
77	4142.0
78	3211.5
79	2281.0
80	1756.0
81	1231.0
82	1231.0
83	946.0
84	661.0
85	486.5
86	312.0
87	250.5
88	189.0
89	189.0
90	142.5
91	96.0
92	67.5
93	39.0
94	29.0
95	19.0
96	19.0
97	11.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1664417.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.52634768770922
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.2543402994383	46.49537438589202
2	10.601677819801242	11.985482530303205
3	2.9798844648390674	5.053259559860893
4	1.2430702862668506	2.8106489280712093
5	0.6879469466963373	1.944356414982756
6	0.42189961816005567	1.430906670325624
7	0.28548630996979285	1.1296248892203546
8	0.21037278342666013	0.9513284080005233
9	0.1595621020437671	0.8117516572116946
>10	1.01259301954169	10.828786384124518
>50	0.0853942327603694	3.3405544400476264
>100	0.05043118806938085	5.1312770977781845
>500	0.0041492207315138125	1.6343850158860478
>1k	0.0027661471543425414	3.1038701637381125
>5k	1.0639027516702083E-4	0.4198271257276557
>10k+	3.1917082550106244E-4	2.92856632882963
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	18413	1.1062732476296504	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16839	1.0117056002191758	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13445	0.8077903554217484	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6981	0.41942614140566936	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	4464	0.26820201908536145	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4043	0.2429078770524454	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3720	0.2235016825711345	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3433	0.20625840759857655	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3325	0.1997696490723178	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3122	0.18757318628685	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2102	0.12629046687218407	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2033	0.12214487114707431	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1962	0.11787911322703386	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1930	0.11595651810814236	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1928	0.11583635591321166	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1702	0.10225802788604059	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1674	0.10057575715701055	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1670	0.1003354327671491	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1666	0.10009510837728766	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.0081097465358744E-5	0.0
10	0.0	0.0	0.0	6.0081097465358744E-5	0.0
11	0.0	0.0	0.0	2.4032438986143498E-4	0.0
12	0.0	0.0	0.0	7.209731695843049E-4	0.0
13	0.0	0.0	0.0	8.411353645150223E-4	0.0
14	0.0	0.0	0.0	9.612975594457399E-4	0.0
15	0.0	0.0	0.0	0.0015020274366339685	0.0
16	0.0	0.0	0.0	0.0018625140214261209	0.0
17	0.0	0.0	0.0	0.0029439737758025783	0.0
18	0.0	0.0	0.0	0.003244379263129372	0.0
19	0.0	0.0	0.0	0.003965352432713677	6.0081097465358744E-5
20	0.0	0.0	0.0	0.0057077042592090805	6.0081097465358744E-5
21	0.0	0.0	0.0	0.009492813399526682	1.8024329239607623E-4
22	0.0	0.0	0.0	0.015621085340993273	1.8024329239607623E-4
23	0.0	0.0	0.0	0.018504978019330494	1.8024329239607623E-4
24	0.0	0.0	0.0	0.024993736545589236	1.8024329239607623E-4
25	0.0	0.0	0.0	0.02901917007576827	1.8024329239607623E-4
26	0.0	0.0	0.0	0.03773092920824529	1.8024329239607623E-4
27	0.0	0.0	0.0	0.08603613157039372	1.8024329239607623E-4
28	0.0	0.0	0.0	0.18312718507441345	1.8024329239607623E-4
29	0.0	0.0	0.0	0.30941765194659754	1.8024329239607623E-4
30	0.0	0.0	0.0	0.48617624068968296	1.8024329239607623E-4
31	0.0	0.0	0.0	0.7003653531536869	2.4032438986143498E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5080	0.0	24.035433	1
ACGTTTA	110	3.8546204E-8	18.5	26
TATACCG	90	2.153396E-6	18.5	5
CGTTTAG	70	1.2196139E-4	18.5	26
GTATTGG	445	0.0	17.460672	1
TTGGACC	735	0.0	15.605443	4
GTATCAA	7850	0.0	15.507008	2
GTAAGAC	85	5.365945E-4	15.235294	3
GGACCCT	700	0.0	14.535715	6
TACCGAC	260	0.0	14.230769	7
TAATTCG	105	1.6563345E-4	14.095238	5
ATTGGAC	570	0.0	13.95614	3
TACACTA	160	1.7846105E-7	13.875	5
CTTACTC	160	1.7846105E-7	13.875	3
TTATGCG	190	7.1304385E-9	13.631579	4
ATACCGA	355	0.0	13.549295	6
TGGACCC	740	0.0	13.5	5
GACCCTC	745	0.0	13.409395	7
CGTTTAT	155	1.888764E-6	13.129032	27
ACAGTCC	230	5.7661964E-10	12.869565	8
>>END_MODULE