FastQCFastQC Report
Wed 25 May 2016
SRR2088706_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088706_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701366
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT82041.1697173800840075No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT73001.040826045174702No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT58390.8325182572294636No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32370.4615279326343164No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG20130.2870113464296815No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG16070.22912430884873236No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA15040.2144386810880482No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA14810.21115936615119638No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA14500.20673941993196135No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG12630.18007716370625323No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA9600.13687575388598822No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA9530.13587770151390288No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9100.12974680837109298No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC8720.12432880977977262No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8320.11862565336785644No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8180.11662954862368578No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA8030.1144908649692172No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA7900.11263733913534446No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG7400.10550839362044925No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGTG400.001930440223.1250027
CGATACC400.001930440223.1250024
ACGTTTA502.7006472E-422.226
GGTATCA24250.021.4371131
GTATTGG2500.019.9800011
ATTGGAC2500.019.2400023
CATATAG609.231924E-418.53
CCGATAC500.00703221518.53
TAGACCA500.00703221518.54
TGCGGTC500.00703221518.512
TGCGGTA500.00703221518.536
CAGGGCG500.00703221518.54
CTAGTCA801.6152058E-518.54
TTAACGG801.6152058E-518.535
TATCGCC500.00703221518.531
TAGTCAG1002.8709474E-718.55
GTATTAG1502.5102054E-1017.2666661
TGGGCTA650.001579103617.07692310
GGACAGT1352.2148015E-816.4444456
GGACCCT3850.016.3376626
Produced by FastQC (version 0.11.5)