##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088706_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	701366
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.570409743272414	31.0	31.0	34.0	30.0	34.0
2	31.781864247767928	31.0	31.0	34.0	30.0	34.0
3	31.86553525548715	31.0	31.0	34.0	30.0	34.0
4	35.573941422880495	37.0	35.0	37.0	33.0	37.0
5	35.455990167758344	37.0	35.0	37.0	33.0	37.0
6	35.49943823909343	37.0	35.0	37.0	33.0	37.0
7	35.3593844012969	37.0	35.0	37.0	33.0	37.0
8	35.396829900508436	37.0	35.0	37.0	33.0	37.0
9	36.98908273283849	39.0	37.0	39.0	33.0	39.0
10	36.81145935217846	39.0	37.0	39.0	32.0	39.0
11	36.901911412871456	39.0	37.0	39.0	33.0	39.0
12	36.764240068666005	39.0	37.0	39.0	32.0	39.0
13	36.7964457929241	39.0	37.0	39.0	32.0	39.0
14	37.7759158556303	40.0	37.0	41.0	32.0	41.0
15	37.798891021235704	40.0	37.0	41.0	32.0	41.0
16	37.68838951417662	39.0	37.0	41.0	32.0	41.0
17	37.71629648428923	39.0	37.0	41.0	32.0	41.0
18	37.67506123764197	39.0	37.0	41.0	32.0	41.0
19	37.734706273186895	40.0	37.0	41.0	32.0	41.0
20	37.61513959901107	39.0	37.0	41.0	32.0	41.0
21	37.551044675675755	39.0	36.0	41.0	32.0	41.0
22	37.423734540881654	39.0	36.0	41.0	32.0	41.0
23	37.23989899709994	39.0	36.0	41.0	31.0	41.0
24	37.07508775731929	39.0	36.0	41.0	31.0	41.0
25	37.26046743069952	39.0	36.0	41.0	31.0	41.0
26	37.143057975436506	39.0	36.0	41.0	31.0	41.0
27	37.02578824750558	39.0	36.0	41.0	31.0	41.0
28	36.855892643783704	39.0	35.0	41.0	30.0	41.0
29	36.74410650074284	39.0	35.0	41.0	30.0	41.0
30	36.54440477582318	39.0	35.0	40.0	30.0	41.0
31	36.38827659167966	38.0	35.0	40.0	30.0	41.0
32	36.20403327221451	38.0	35.0	40.0	30.0	41.0
33	36.051679151826576	38.0	35.0	40.0	30.0	41.0
34	35.86142185392505	38.0	35.0	40.0	29.0	41.0
35	35.720397053749394	38.0	35.0	40.0	27.0	41.0
36	35.51837129259189	38.0	34.0	40.0	27.0	41.0
37	35.43210677449434	38.0	34.0	40.0	26.0	41.0
38	35.238808268436166	38.0	34.0	40.0	25.0	41.0
39	35.1413798216623	38.0	34.0	40.0	25.0	41.0
40	34.97387669205522	38.0	34.0	40.0	24.0	41.0
41	34.8284675903879	38.0	34.0	40.0	23.0	41.0
42	34.55718697513139	38.0	33.0	40.0	22.0	41.0
43	34.20496716407696	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	5.0
16	11.0
17	12.0
18	38.0
19	106.0
20	232.0
21	491.0
22	932.0
23	1635.0
24	2726.0
25	4143.0
26	6023.0
27	8792.0
28	11897.0
29	15294.0
30	19367.0
31	24043.0
32	29304.0
33	35904.0
34	44263.0
35	54280.0
36	69688.0
37	96353.0
38	128120.0
39	147705.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.65485638026365	21.394393226931445	13.756583581183005	23.19416681162189
2	18.76324201629392	23.6019424950739	34.907166871505034	22.72764861712715
3	19.89175409130183	24.812722601323703	32.5690153215297	22.726507985844766
4	13.679163232891245	16.55811658962653	36.64506120912619	33.11765896835603
5	12.666710390865823	38.66597468368869	35.1678011195296	13.499513805915884
6	31.993281681746762	37.53817550323226	13.958190160344241	16.51035265467673
7	25.80350344898384	33.12250665130617	21.968415919790807	19.105573979919185
8	26.849747492749863	34.476578562405365	19.452753626494584	19.220920318350192
9	24.48079889815018	14.640002509388822	22.223489590313758	38.65570900214724
10	16.197391946572832	27.83297165816421	34.00521268496049	21.964423710302466
11	32.51611854580918	22.878354525312034	23.828500383537268	20.77702654534152
12	20.6491332628043	27.419635397210584	31.490405865126053	20.44082547485906
13	30.998793782418883	21.186370596806803	26.63345528582794	21.181380334946375
14	20.264455362820552	23.271444581003355	28.350960839276496	28.11313921689959
15	22.60930812157989	29.85388513272671	25.702700159403218	21.834106586290183
16	21.416350379117322	28.67247628199827	27.13576078680746	22.775412552076947
17	21.096545883319124	27.306855479164945	27.75426809967977	23.842330537836165
18	22.420961381076356	26.22425381327296	29.875271969271395	21.479512836379293
19	22.099873675085476	25.60959613097869	30.997082835495306	21.293447358440527
20	23.202864125150064	25.262986800044484	30.69980580752417	20.834343267281277
21	21.87716541720015	26.098071477659314	31.274826552755624	20.749936552384916
22	20.831206531254722	26.372678458893073	30.74785490029457	22.048260109557635
23	21.59742559519566	26.620195447170236	31.503808282694056	20.278570674940045
24	21.73430134908165	26.681504378598337	29.462506023958962	22.121688248361053
25	21.579032915767232	26.890810218915657	30.394259202755762	21.135897662561344
26	21.449999001947628	27.460983281196977	30.32724711491575	20.761770601939645
27	20.34444213149768	27.64248623400621	30.1197948004323	21.893276834063812
28	20.89265804159312	27.154153466235886	30.31983301158026	21.633355480590733
29	21.13689571493343	27.140465890847288	29.7861601503352	21.93647824388408
30	21.225579797138728	27.500192481528902	30.97156121055198	20.302666510780394
31	21.781494968390255	27.144315521425334	30.782358996586662	20.29183051359775
32	21.366447760513054	27.078444064867703	29.906354171716337	21.648754002902905
33	21.051348368754688	26.962384831885206	30.584887205824064	21.401379593536042
34	20.334176449956228	27.427334658366675	31.103874439308438	21.134614452368663
35	19.992842538703044	29.203582722857966	30.03068298149611	20.77289175694288
36	21.917515248814457	27.2826170644143	29.90136390985591	20.898503776915334
37	21.092125937099887	27.769524043081645	29.807832144700484	21.330517875117984
38	20.829067847600253	26.597240242612273	30.969279947987218	21.604411961800256
39	20.24691815685391	26.427713918268065	31.697573021788912	21.627794903089114
40	19.559687809218012	26.940570258609625	31.29906496750627	22.200676964666094
41	20.97435575719382	25.846990016624698	31.20981056965978	21.968843656521702
42	19.760296336007162	27.58958945828569	30.67699318187651	21.97312102383064
43	20.076393780137618	27.35832646578249	30.130060481973747	22.435219272106146
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	213.0
1	204.0
2	195.0
3	416.0
4	637.0
5	637.0
6	964.0
7	1291.0
8	1423.5
9	1556.0
10	2369.5
11	3183.0
12	3183.0
13	5893.0
14	8603.0
15	13066.5
16	17530.0
17	16221.0
18	14912.0
19	14912.0
20	16142.5
21	17373.0
22	14346.5
23	11320.0
24	12810.0
25	14300.0
26	14300.0
27	15732.5
28	17165.0
29	18500.0
30	19835.0
31	21856.5
32	23878.0
33	23878.0
34	26143.0
35	28408.0
36	30715.0
37	33022.0
38	34543.5
39	36065.0
40	36065.0
41	37022.5
42	37980.0
43	39659.5
44	41339.0
45	43982.5
46	46626.0
47	46626.0
48	58278.5
49	69931.0
50	65362.0
51	60793.0
52	55233.5
53	49674.0
54	49674.0
55	44691.0
56	39708.0
57	34003.0
58	28298.0
59	25253.0
60	22208.0
61	22208.0
62	19696.5
63	17185.0
64	14788.5
65	12392.0
66	10726.0
67	9060.0
68	9060.0
69	7651.0
70	6242.0
71	5203.0
72	4164.0
73	3417.0
74	2670.0
75	2670.0
76	2161.0
77	1652.0
78	1278.0
79	904.0
80	701.0
81	498.0
82	498.0
83	383.5
84	269.0
85	207.5
86	146.0
87	108.0
88	70.0
89	70.0
90	56.5
91	43.0
92	28.0
93	13.0
94	11.0
95	9.0
96	9.0
97	6.5
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	701366.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.15808920687679
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.20118240003117	55.07460056966303
2	7.705454127089638	9.733235182764485
3	1.9139692884170956	3.6264792917120823
4	0.8515872429142627	2.1513849222166903
5	0.4977948974151177	1.571988726883604
6	0.32218938558026405	1.220931957359228
7	0.24282539968270836	1.0735471778399204
8	0.16816184738051082	0.8496624766441202
9	0.13919271186655938	0.7912031141713731
>10	0.8447706518138131	9.896576630673971
>50	0.06989482346301967	3.00823426124791
>100	0.037096130488232903	4.4989792237240565
>500	0.003619134681642366	1.6604736287395097
>1k	0.001583371423218535	1.7936200988406217
>5k	6.785877528079435E-4	3.049082737519346
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8204	1.1697173800840075	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7300	1.040826045174702	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5839	0.8325182572294636	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3237	0.4615279326343164	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2013	0.2870113464296815	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1607	0.22912430884873236	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1504	0.2144386810880482	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1481	0.21115936615119638	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1450	0.20673941993196135	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1263	0.18007716370625323	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	960	0.13687575388598822	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	953	0.13587770151390288	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	910	0.12974680837109298	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	872	0.12432880977977262	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	832	0.11862565336785644	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	818	0.11662954862368578	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	803	0.1144908649692172	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	790	0.11263733913534446	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	740	0.10550839362044925	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.4257891029790439E-4	0.0
10	0.0	0.0	0.0	1.4257891029790439E-4	0.0
11	0.0	0.0	0.0	1.4257891029790439E-4	0.0
12	0.0	0.0	0.0	2.8515782059580877E-4	0.0
13	0.0	0.0	0.0	4.2773673089371313E-4	0.0
14	0.0	0.0	0.0	5.703156411916175E-4	0.0
15	0.0	0.0	0.0	8.554734617874263E-4	0.0
16	0.0	0.0	0.0	0.0021386836544685654	0.0
17	0.0	0.0	0.0	0.0029941571162559917	0.0
18	0.0	0.0	0.0	0.003421893847149705	0.0
19	0.0	0.0	0.0	0.004419946219235036	0.0
20	0.0	0.0	0.0	0.007556682245788932	0.0
21	0.0	0.0	0.0	0.013117259747407203	1.4257891029790439E-4
22	0.0	0.0	0.0	0.01996104744170661	1.4257891029790439E-4
23	0.0	0.0	0.0	0.02409583584034584	1.4257891029790439E-4
24	0.0	0.0	0.0	0.03279314936851801	1.4257891029790439E-4
25	0.0	0.0	0.0	0.0392092003319237	1.4257891029790439E-4
26	0.0	0.0	0.0	0.050615513155756055	1.4257891029790439E-4
27	0.0	0.0	0.0	0.09709623791287288	1.4257891029790439E-4
28	0.0	0.0	0.0	0.20873552467613202	1.4257891029790439E-4
29	0.0	0.0	0.0	0.3409061745222894	1.4257891029790439E-4
30	0.0	0.0	0.0	0.5202704436770531	1.4257891029790439E-4
31	0.0	0.0	0.0	0.7334259145724201	1.4257891029790439E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCGTG	40	0.0019304402	23.125002	7
CGATACC	40	0.0019304402	23.125002	4
ACGTTTA	50	2.7006472E-4	22.2	26
GGTATCA	2425	0.0	21.437113	1
GTATTGG	250	0.0	19.980001	1
ATTGGAC	250	0.0	19.240002	3
CATATAG	60	9.231924E-4	18.5	3
CCGATAC	50	0.007032215	18.5	3
TAGACCA	50	0.007032215	18.5	4
TGCGGTC	50	0.007032215	18.5	12
TGCGGTA	50	0.007032215	18.5	36
CAGGGCG	50	0.007032215	18.5	4
CTAGTCA	80	1.6152058E-5	18.5	4
TTAACGG	80	1.6152058E-5	18.5	35
TATCGCC	50	0.007032215	18.5	31
TAGTCAG	100	2.8709474E-7	18.5	5
GTATTAG	150	2.5102054E-10	17.266666	1
TGGGCTA	65	0.0015791036	17.076923	10
GGACAGT	135	2.2148015E-8	16.444445	6
GGACCCT	385	0.0	16.337662	6
>>END_MODULE