FastQCFastQC Report
Wed 25 May 2016
SRR2088705_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088705_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2598838
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT250320.9631997069459504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT224370.8633473883327856No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT177810.684190395861535No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98930.3806701302659111No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG55010.21167152396571082No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG47990.18465945164723618No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA44180.1699990534231068No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA42960.16530464769254566No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA38680.14883574890008536No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG38610.14856639775161054No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC26620.10243039389142379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68250.022.850551
GTATTGG8000.019.88751
GGACCCT10550.017.360196
TGGACCC11750.017.1617035
TTGGACC11900.016.945384
ATTGGAC9400.016.9255333
GACCCTC10500.016.2095247
TTAACGG2500.015.54000135
GGACCGT2500.015.5400016
AGTACCG2400.015.4166665
GTATTAG5950.015.2352941
AGACGCG750.004106257614.814
GTCGTAG1001.09420645E-414.81
GTATCAA105700.014.7019862
TGTTACG1304.448868E-614.23076916
TAGTACC2252.7284841E-1113.97777754
GTCGAGT800.006301440813.8751
ACCGTTA1907.1377144E-913.6315798
TAACGCC2051.4260877E-913.5365864
TATTGGA10700.013.4859812
Produced by FastQC (version 0.11.5)