FastQCFastQC Report
Wed 25 May 2016
SRR2088704_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088704_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3879384
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT301240.7765150343456589No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT288030.7424632364313509No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT222150.5726424607618117No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125660.32391740544375086No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG77100.19874289320160107No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG63430.1635053400230552No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA59620.15368419316056364No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA55550.14319283680089415No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA53690.13839826116723686No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG51780.1334747990918146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94000.022.3771271
GTATTGG9450.015.6613761
GTATTAG10200.015.0539221
CTAATAC10200.014.6911763
GTATCAA143950.014.6252182
TCTAATA9450.014.4867732
TAATACT12100.014.21900754
CTCTAAT9850.014.0862951
TATACCG1907.1413524E-913.6315795
TCTATAC4500.013.5666673
GTACTAT4500.013.1555561
TTAACGG4100.013.08536535
ATACGAC1901.0479562E-712.65789519
AATACTG13100.012.5687035
ATTAGAG7550.012.4966893
GGACCCT15700.012.4904466
TGGACCC15850.012.4889585
TACGACT1951.4701982E-712.33333420
TTGGACC17150.012.1895054
CGCCTAT2756.184564E-1112.10909236
Produced by FastQC (version 0.11.5)