##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088704_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3879384
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.565967689715688	31.0	31.0	34.0	30.0	34.0
2	31.778679295475776	31.0	31.0	34.0	30.0	34.0
3	31.86760630038171	31.0	31.0	34.0	30.0	34.0
4	35.58282191193241	37.0	35.0	37.0	33.0	37.0
5	35.447694530884284	37.0	35.0	37.0	33.0	37.0
6	35.4834115416262	37.0	35.0	37.0	33.0	37.0
7	35.35556830672086	37.0	35.0	37.0	33.0	37.0
8	35.36947231828558	37.0	35.0	37.0	33.0	37.0
9	36.97885282818097	39.0	37.0	39.0	33.0	39.0
10	36.78046488823999	39.0	37.0	39.0	32.0	39.0
11	36.870380194381376	39.0	37.0	39.0	33.0	39.0
12	36.731233876306135	39.0	35.0	39.0	32.0	39.0
13	36.76885763306752	39.0	37.0	39.0	32.0	39.0
14	37.78814574685053	40.0	37.0	41.0	32.0	41.0
15	37.799293908517434	40.0	37.0	41.0	32.0	41.0
16	37.69156134066645	39.0	37.0	41.0	32.0	41.0
17	37.68609114230507	39.0	37.0	41.0	32.0	41.0
18	37.63703438484048	39.0	36.0	41.0	32.0	41.0
19	37.6711673812131	40.0	37.0	41.0	32.0	41.0
20	37.543299400110946	39.0	36.0	41.0	32.0	41.0
21	37.4793281613782	39.0	36.0	41.0	32.0	41.0
22	37.34615392546858	39.0	36.0	41.0	31.0	41.0
23	37.169765612272464	39.0	36.0	41.0	31.0	41.0
24	37.01834363393776	39.0	36.0	41.0	31.0	41.0
25	37.20672405722146	39.0	36.0	41.0	31.0	41.0
26	37.09389403059867	39.0	36.0	41.0	31.0	41.0
27	36.9934061696393	39.0	36.0	41.0	31.0	41.0
28	36.8352369860782	39.0	36.0	41.0	30.0	41.0
29	36.737269370601105	39.0	35.0	41.0	30.0	41.0
30	36.564785285498935	39.0	35.0	40.0	30.0	41.0
31	36.451334026226846	39.0	35.0	40.0	30.0	41.0
32	36.29016513962011	38.0	35.0	40.0	30.0	41.0
33	36.173842805971255	38.0	35.0	40.0	30.0	41.0
34	36.003075230500514	38.0	35.0	40.0	29.0	41.0
35	35.89225454350485	38.0	35.0	40.0	28.0	41.0
36	35.72102426570817	38.0	35.0	40.0	27.0	41.0
37	35.68370674313241	38.0	35.0	40.0	27.0	41.0
38	35.52036766661924	38.0	34.0	40.0	26.0	41.0
39	35.453710692212994	38.0	34.0	40.0	26.0	41.0
40	35.324680155406114	38.0	34.0	40.0	25.0	41.0
41	35.23466947329782	38.0	34.0	40.0	25.0	41.0
42	35.02538830907175	38.0	34.0	40.0	24.0	41.0
43	34.69786259880435	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	1.0
13	5.0
14	13.0
15	20.0
16	38.0
17	117.0
18	285.0
19	629.0
20	1441.0
21	2863.0
22	5249.0
23	9395.0
24	15223.0
25	22755.0
26	33400.0
27	46635.0
28	62538.0
29	82466.0
30	104959.0
31	129523.0
32	159608.0
33	195184.0
34	240157.0
35	295199.0
36	378569.0
37	514538.0
38	725925.0
39	852646.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.38793684770572	21.072443460095727	13.203230203558093	24.336389488640464
2	18.915863961907352	22.926758475056864	34.64488176473378	23.512495798301998
3	19.55264031609142	24.640097500015468	31.673224408823668	24.134037775069444
4	13.904011564722646	16.55087508738501	36.3613913961598	33.183721951732544
5	13.09329006873256	38.13435328907889	35.088998665767555	13.683357976420998
6	32.77690478694556	37.27738733778352	14.314746877339287	15.63096099793163
7	26.819129016359298	32.19792111324891	22.050717330380287	18.932232540011505
8	26.61791150347581	34.76541636507239	19.48399539720739	19.132676734244406
9	25.019590739148278	14.386407738960619	21.21512075112956	39.37888077076154
10	16.00957780926044	27.619616928873242	34.38234008285851	21.9884651790078
11	33.6930553922994	22.426653303720386	23.264956498248175	20.615334805732044
12	20.65531537996754	26.803018211138674	31.254085700203948	21.28758070868983
13	30.706498763721253	21.141990584072108	25.889806216657078	22.26170443554956
14	20.74888693668892	22.36081810926683	27.410382679311972	29.47991227473228
15	23.23023449083669	29.313158996376742	24.805510359376644	22.651096153409924
16	22.39445747056749	28.19256871709529	26.23205127411981	23.18092253821741
17	21.965420283220222	27.06442569232641	27.049397533216613	23.92075649123675
18	22.845792012340105	26.302655266918666	28.67151073469396	22.180041986047268
19	22.876106103443227	25.551041093122002	29.578793952854372	21.9940588505804
20	23.662493839228084	25.262026136108208	29.235672467587637	21.83980755707607
21	22.580672601629537	26.07679466636971	29.55760502182821	21.784927710172543
22	21.98140220199908	26.214728936346592	29.229331254652802	22.574537607001524
23	22.45562697582915	26.355885367367605	29.7571212336804	21.431366423122846
24	22.595829647181098	26.422210330299862	28.104358836351338	22.877601186167702
25	22.615832822943023	26.375424551939176	28.875512194719573	22.13323043039823
26	22.298050412127285	26.917701367021156	28.874145998436866	21.910102222414693
27	21.646529449005307	26.98206725603859	28.637175386607773	22.734227908348338
28	21.91868605943624	26.698130424830335	28.926783221253682	22.456400294479742
29	22.228348624420786	26.598501205346004	28.568350026705268	22.604800143527942
30	22.248583795777886	26.687999950507606	29.43160563635876	21.631810617355747
31	22.63851683669366	26.581874854358322	29.23062011907045	21.54898818987757
32	22.221878525044183	26.42857732052305	28.763664540555922	22.58587961387684
33	21.966322488312578	26.384704375746253	29.235492026569165	22.413481109372
34	21.710018910218736	26.573110576318303	29.615243038585508	22.101627474877457
35	21.461989841686204	27.676352740538192	28.911007520781652	21.95064989699396
36	22.778848394487373	26.343331827939693	28.760725929683684	22.11709384788925
37	22.00411199303807	26.49351030988425	28.973105008424017	22.529272688653663
38	21.907447161714334	25.66314652016918	29.906088183072367	22.52331813504412
39	21.42347857288683	25.541838601179983	30.398382836037886	22.636299989895303
40	20.90868034718914	25.845443503401572	30.32185006691784	22.924026082491448
41	21.741415647432685	25.000283550171883	30.307930331207224	22.950370471188208
42	20.480880469682816	26.47353291141068	30.041341615060535	23.004245003845973
43	21.068679975996186	25.988017685281996	29.613335519247386	23.329966819474432
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1352.0
1	1220.0
2	1088.0
3	1925.0
4	2762.0
5	2762.0
6	4184.0
7	5606.0
8	6079.0
9	6552.0
10	9667.5
11	12783.0
12	12783.0
13	23183.0
14	33583.0
15	49707.0
16	65831.0
17	62915.0
18	59999.0
19	59999.0
20	66354.5
21	72710.0
22	63380.0
23	54050.0
24	62100.0
25	70150.0
26	70150.0
27	80140.5
28	90131.0
29	98603.5
30	107076.0
31	119429.5
32	131783.0
33	131783.0
34	146507.5
35	161232.0
36	173673.5
37	186115.0
38	197092.5
39	208070.0
40	208070.0
41	213940.0
42	219810.0
43	228836.0
44	237862.0
45	247325.0
46	256788.0
47	256788.0
48	303123.0
49	349458.0
50	332524.5
51	315591.0
52	294049.5
53	272508.0
54	272508.0
55	253475.0
56	234442.0
57	206636.5
58	178831.0
59	163608.0
60	148385.0
61	148385.0
62	132442.0
63	116499.0
64	102251.0
65	88003.0
66	76365.5
67	64728.0
68	64728.0
69	55166.0
70	45604.0
71	39051.5
72	32499.0
73	26539.0
74	20579.0
75	20579.0
76	16512.0
77	12445.0
78	9639.5
79	6834.0
80	5332.5
81	3831.0
82	3831.0
83	2875.0
84	1919.0
85	1440.0
86	961.0
87	741.5
88	522.0
89	522.0
90	384.5
91	247.0
92	179.5
93	112.0
94	73.5
95	35.0
96	35.0
97	22.0
98	9.0
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3879384.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.46242511222117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.55823474438596	39.13776612624646
2	12.448945749558842	12.564079852264342
3	4.179627022876154	6.327423469167115
4	1.8766931629710517	3.7880995278017666
5	0.9966032228836009	2.514550775068099
6	0.5940132907162409	1.7985210719059417
7	0.408506789157617	1.4429970278990119
8	0.28124974298278105	1.135403527448003
9	0.20739593704198664	0.9419131747404167
>10	1.2518490993370828	11.982826500279511
>50	0.12075154075830245	4.199405032589491
>100	0.06763240390894447	6.500202460226095
>500	0.005118851473495842	1.7965695071161643
>1k	0.0028665568043812124	2.516736821744117
>5k	3.071310861837013E-4	0.9329369891608655
>10k+	2.0475405745580086E-4	2.4205681363426192
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	30124	0.7765150343456589	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	28803	0.7424632364313509	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	22215	0.5726424607618117	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12566	0.32391740544375086	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7710	0.19874289320160107	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6343	0.1635053400230552	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5962	0.15368419316056364	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5555	0.14319283680089415	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5369	0.13839826116723686	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5178	0.1334747990918146	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.577728835299625E-5	0.0	0.0	0.0	0.0
6	2.577728835299625E-5	0.0	0.0	0.0	0.0
7	2.577728835299625E-5	0.0	0.0	2.577728835299625E-5	0.0
8	2.577728835299625E-5	0.0	0.0	5.15545767059925E-5	0.0
9	1.2888644176498124E-4	0.0	0.0	1.8044101847097374E-4	0.0
10	1.546637301179775E-4	0.0	0.0	2.577728835299625E-4	0.0
11	2.0621830682397E-4	2.577728835299625E-5	0.0	3.8665932529494373E-4	0.0
12	2.0621830682397E-4	2.577728835299625E-5	0.0	5.15545767059925E-4	0.0
13	2.0621830682397E-4	2.577728835299625E-5	0.0	6.1865492047191E-4	0.0
14	2.0621830682397E-4	2.577728835299625E-5	0.0	6.444322088249063E-4	0.0
15	2.0621830682397E-4	2.577728835299625E-5	0.0	7.733186505898875E-4	0.0
16	2.3199559517696623E-4	2.577728835299625E-5	0.0	0.0013404189943558048	0.0
17	2.3199559517696623E-4	2.577728835299625E-5	0.0	0.001881742049768726	0.0
18	2.3199559517696623E-4	2.577728835299625E-5	0.0	0.0023972878168286513	0.0
19	3.351047485889512E-4	2.577728835299625E-5	0.0	0.003015942737300561	0.0
20	3.351047485889512E-4	2.577728835299625E-5	0.0	0.004768798345304306	0.0
21	3.351047485889512E-4	2.577728835299625E-5	0.0	0.007681631929192882	0.0
22	3.351047485889512E-4	2.577728835299625E-5	0.0	0.012244211967673219	0.0
23	3.351047485889512E-4	2.577728835299625E-5	0.0	0.015337486570032768	0.0
24	3.6088203694194747E-4	2.577728835299625E-5	0.0	0.020776494412514977	0.0
25	3.6088203694194747E-4	2.577728835299625E-5	0.0	0.02557107004617228	0.0
26	3.6088203694194747E-4	2.577728835299625E-5	0.0	0.03351047485889512	0.0
27	3.8665932529494373E-4	2.577728835299625E-5	0.0	0.08346685968700185	0.0
28	3.8665932529494373E-4	2.577728835299625E-5	0.0	0.18508093037451306	0.0
29	3.8665932529494373E-4	2.577728835299625E-5	0.0	0.30133650084652613	0.0
30	3.8665932529494373E-4	2.577728835299625E-5	0.0	0.4639911903539325	0.0
31	3.8665932529494373E-4	2.577728835299625E-5	0.0	0.6828661457592237	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9400	0.0	22.377127	1
GTATTGG	945	0.0	15.661376	1
GTATTAG	1020	0.0	15.053922	1
CTAATAC	1020	0.0	14.691176	3
GTATCAA	14395	0.0	14.625218	2
TCTAATA	945	0.0	14.486773	2
TAATACT	1210	0.0	14.2190075	4
CTCTAAT	985	0.0	14.086295	1
TATACCG	190	7.1413524E-9	13.631579	5
TCTATAC	450	0.0	13.566667	3
GTACTAT	450	0.0	13.155556	1
TTAACGG	410	0.0	13.085365	35
ATACGAC	190	1.0479562E-7	12.657895	19
AATACTG	1310	0.0	12.568703	5
ATTAGAG	755	0.0	12.496689	3
GGACCCT	1570	0.0	12.490446	6
TGGACCC	1585	0.0	12.488958	5
TACGACT	195	1.4701982E-7	12.333334	20
TTGGACC	1715	0.0	12.189505	4
CGCCTAT	275	6.184564E-11	12.109092	36
>>END_MODULE