FastQCFastQC Report
Wed 25 May 2016
SRR2088703_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088703_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3125888
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT363051.161429968060276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT335931.0746706215961672No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT255300.8167279185946521No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138670.4436179415257361No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG77280.2472257483313542No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG76330.24418661193235328No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA71730.22947079357929653No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA60910.19485662954014987No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG59410.19005799312067484No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA53740.17191914745505915No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA42930.1373369743253757No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT40530.12965915605421563No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC39170.12530839236722494No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37930.12134151959379223No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA35040.11209614675893698No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33450.10700959215429343No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA32040.1024988739199869No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA31800.1017310920928709No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103800.024.22111
GTATTGG7250.016.5862081
GTATCAA158200.015.8922252
TTGGACC13250.014.9396234
TGGACCC13650.014.5018315
TATCGGC1152.2121392E-514.478262
GGACCCT13200.014.4356076
CGCGATA1458.923307E-714.03448314
ATTGGAC9100.014.02747253
ACGTTTA2151.9826984E-1013.76744226
TAGGACC4050.013.7037044
GTATTAG4600.013.6739121
CTAATAC5850.013.5982913
TTAACGG2900.013.39655135
TATACCG1805.1679308E-813.361115
GACCCTC14000.013.3464287
CGACGAA2651.8189894E-1213.26415210
TATACAC15800.012.99683537
TACACCG2152.904926E-912.9069775
TCTATAC2753.6379788E-1212.7818173
Produced by FastQC (version 0.11.5)