##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088703_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3125888
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53707394506777	31.0	31.0	34.0	30.0	34.0
2	31.746092950227265	31.0	31.0	34.0	30.0	34.0
3	31.81818734388436	31.0	31.0	34.0	30.0	34.0
4	35.539774297735555	37.0	35.0	37.0	33.0	37.0
5	35.413313272900375	37.0	35.0	37.0	33.0	37.0
6	35.46481479822694	37.0	35.0	37.0	33.0	37.0
7	35.32571064606282	37.0	35.0	37.0	32.0	37.0
8	35.36096782738217	37.0	35.0	37.0	33.0	37.0
9	36.94896458222431	39.0	37.0	39.0	33.0	39.0
10	36.764965667355966	39.0	37.0	39.0	32.0	39.0
11	36.852346597190945	39.0	37.0	39.0	32.0	39.0
12	36.712108367286355	39.0	35.0	39.0	32.0	39.0
13	36.752874383215264	39.0	35.0	39.0	32.0	39.0
14	37.7337076696286	40.0	37.0	41.0	32.0	41.0
15	37.743509044469924	40.0	37.0	41.0	32.0	41.0
16	37.6327469186356	39.0	36.0	41.0	32.0	41.0
17	37.66153553806151	39.0	37.0	41.0	32.0	41.0
18	37.6217877927808	39.0	36.0	41.0	32.0	41.0
19	37.673752866385485	39.0	37.0	41.0	32.0	41.0
20	37.54836641619917	39.0	36.0	41.0	32.0	41.0
21	37.49344986128742	39.0	36.0	41.0	32.0	41.0
22	37.36094543374555	39.0	36.0	41.0	31.0	41.0
23	37.189060516563615	39.0	36.0	41.0	31.0	41.0
24	37.026498390217434	39.0	36.0	41.0	31.0	41.0
25	37.204961918021375	39.0	36.0	41.0	31.0	41.0
26	37.08412777425167	39.0	36.0	41.0	31.0	41.0
27	36.96875671809099	39.0	36.0	41.0	31.0	41.0
28	36.79560048216699	39.0	35.0	41.0	30.0	41.0
29	36.6867850031735	39.0	35.0	40.0	30.0	41.0
30	36.48937837824004	39.0	35.0	40.0	30.0	41.0
31	36.3399238872282	38.0	35.0	40.0	30.0	41.0
32	36.1686391195078	38.0	35.0	40.0	30.0	41.0
33	36.03442093894599	38.0	35.0	40.0	29.0	41.0
34	35.85398549148274	38.0	35.0	40.0	28.0	41.0
35	35.7274681626469	38.0	35.0	40.0	27.0	41.0
36	35.53475716340445	38.0	34.0	40.0	27.0	41.0
37	35.46890003736538	38.0	34.0	40.0	26.0	41.0
38	35.27452966964907	38.0	34.0	40.0	25.0	41.0
39	35.18763884054707	38.0	34.0	40.0	25.0	41.0
40	35.021191738155686	38.0	34.0	40.0	24.0	41.0
41	34.891596563920395	38.0	34.0	40.0	24.0	41.0
42	34.642294925473976	38.0	33.0	40.0	23.0	41.0
43	34.283275664387205	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	6.0
14	7.0
15	15.0
16	40.0
17	89.0
18	206.0
19	518.0
20	1119.0
21	2414.0
22	4468.0
23	7704.0
24	12481.0
25	19371.0
26	28480.0
27	39257.0
28	53245.0
29	68906.0
30	87192.0
31	107412.0
32	131958.0
33	161214.0
34	196773.0
35	241221.0
36	310198.0
37	423896.0
38	575433.0
39	652262.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.725515437533275	21.731200861963064	14.269097293313132	23.274186407190534
2	18.96101203881905	23.54690891036403	34.8802324331518	22.611846617665122
3	19.731129202325867	24.593875404365097	32.67148407108636	23.00351132222268
4	13.980315353589123	16.812502559272758	35.9853584005569	33.22182368658122
5	12.670799465623848	38.68260795012489	34.92220450636747	13.72438807788379
6	32.23883901150649	37.31189345235658	13.898162698087713	16.55110483804922
7	25.72344242660006	33.26043671430327	21.743517362106385	19.272603496990296
8	26.63675090086401	34.89059748781786	19.197296896114	19.275354715204127
9	24.527814176323655	14.593709051635887	22.016431810736663	38.862044961303795
10	16.30259945333934	28.08629099954957	33.72372906514885	21.887380481962246
11	32.97677332009336	22.75475001023709	23.49124472789812	20.777231941771426
12	20.69379964989149	27.470465992383602	31.151851889767002	20.683882467957908
13	31.09593178002539	21.363561330412352	26.344130052004424	21.19637683755784
14	20.24282379918922	23.30083483477335	28.09249723598542	28.363844130052
15	22.66079270914377	29.86165851111748	25.582298534048565	21.895250245690185
16	21.53858359608534	28.546960095819173	26.967121022890133	22.947335285205355
17	21.348877502968758	27.443913537529173	27.45034371033127	23.756865249170794
18	22.51158710740756	26.195660241185863	29.655285154170592	21.637467497235985
19	22.460209706809714	25.41844109577822	30.94291926006306	21.178429937349
20	23.2300069612219	25.003710945497726	30.695373602637076	21.070908490643298
21	22.1184508210147	25.893090219483234	31.169734808156914	20.81872415134515
22	21.03475876294992	26.169427695426066	30.758203748822734	22.037609792801277
23	21.924649891486837	26.293552424143158	31.32607438270341	20.4557233016666
24	21.79390304451087	26.458209635150077	29.412954014987104	22.33493330535195
25	21.703144834363865	26.638318455427708	30.49251924573113	21.166017464477292
26	21.45579112239466	27.276025244666474	30.249132406535356	21.019051226403505
27	20.583846894066582	27.232229689611398	30.073630277220424	22.110293139101593
28	21.01626801728021	26.81698768477949	30.213334578846073	21.95340971909422
29	21.28892653863478	26.72571762008108	29.67972620900045	22.30562963228369
30	21.380612485156217	26.949014168133985	31.02561576102535	20.644757585684452
31	22.01691167437861	26.68281781049097	30.683728911592485	20.61654160353794
32	21.46740382252979	26.67427623766431	29.979960894312274	21.878359045493635
33	21.208533383153842	26.47954757176201	30.516128536914948	21.795790508169198
34	20.48169992015069	26.84270837598788	31.219512663281602	21.45607904057983
35	20.27423887228205	28.27033470169117	30.22114036075509	21.234286065271693
36	22.108437666352728	26.413102452807006	30.115282441341467	21.36317743949879
37	21.16397004627165	27.00416649604848	30.185758414888824	21.646105042791042
38	21.032167499283403	25.78470501822202	31.35636977396503	21.826757708529545
39	20.591556703247203	25.56236819745301	31.96253352647312	21.883541572826665
40	19.646417274067403	26.03640949387822	31.731015314688175	22.5861579173662
41	20.748152205069406	25.27345829409115	31.70871765079235	22.26967185004709
42	19.701217701977804	26.946230959010688	31.09468413455632	22.25786720445518
43	19.982257841611727	26.726165492813564	30.55007089185537	22.74150577371934
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1309.0
1	1146.5
2	984.0
3	1791.5
4	2599.0
5	2599.0
6	3929.5
7	5260.0
8	5844.0
9	6428.0
10	9889.0
11	13350.0
12	13350.0
13	24908.0
14	36466.0
15	56045.5
16	75625.0
17	69770.0
18	63915.0
19	63915.0
20	69216.0
21	74517.0
22	60764.5
23	47012.0
24	52592.0
25	58172.0
26	58172.0
27	65098.0
28	72024.0
29	78061.5
30	84099.0
31	93191.0
32	102283.0
33	102283.0
34	113828.5
35	125374.0
36	136094.0
37	146814.0
38	155140.5
39	163467.0
40	163467.0
41	168661.5
42	173856.0
43	182450.0
44	191044.0
45	202282.0
46	213520.0
47	213520.0
48	265179.5
49	316839.0
50	293216.0
51	269593.0
52	244220.5
53	218848.0
54	218848.0
55	197496.5
56	176145.0
57	153248.5
58	130352.0
59	117199.5
60	104047.0
61	104047.0
62	91923.0
63	79799.0
64	69032.5
65	58266.0
66	49207.5
67	40149.0
68	40149.0
69	34007.5
70	27866.0
71	23206.0
72	18546.0
73	15243.0
74	11940.0
75	11940.0
76	9499.5
77	7059.0
78	5462.0
79	3865.0
80	3042.5
81	2220.0
82	2220.0
83	1678.0
84	1136.0
85	853.0
86	570.0
87	432.0
88	294.0
89	294.0
90	214.0
91	134.0
92	101.0
93	68.0
94	44.0
95	20.0
96	20.0
97	13.5
98	7.0
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3125888.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.261888642741134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.4953797876237	38.175687694248516
2	12.417148720913348	12.233843950999777
3	4.294709748091593	6.346965400901487
4	1.8945165496778702	3.733098532082456
5	1.0035927530719606	2.471943722224646
6	0.6099870899960428	1.8029469660536863
7	0.40424875367111046	1.3939839961119191
8	0.28140580550223837	1.109006516325771
9	0.2106333540416296	0.9338577148121243
>10	1.194001819084375	11.18564216290991
>50	0.11346132777835893	3.902870365725724
>100	0.07155820295866487	6.774364601862749
>500	0.004807989455668185	1.5888837200501917
>1k	0.0038983698287337358	3.570363381108311
>5k	3.8983698287337363E-4	1.2783533253381079
>10k+	2.598913219155824E-4	3.498187949244579
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	36305	1.161429968060276	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	33593	1.0746706215961672	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	25530	0.8167279185946521	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13867	0.4436179415257361	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7728	0.2472257483313542	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7633	0.24418661193235328	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7173	0.22947079357929653	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6091	0.19485662954014987	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5941	0.19005799312067484	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5374	0.17191914745505915	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4293	0.1373369743253757	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4053	0.12965915605421563	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3917	0.12530839236722494	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3793	0.12134151959379223	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3504	0.11209614675893698	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3345	0.10700959215429343	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3204	0.1024988739199869	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3180	0.1017310920928709	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.199090946316694E-5	0.0
3	0.0	0.0	0.0	3.199090946316694E-5	0.0
4	0.0	0.0	0.0	3.199090946316694E-5	0.0
5	3.199090946316694E-5	0.0	0.0	6.398181892633389E-5	0.0
6	3.199090946316694E-5	0.0	0.0	6.398181892633389E-5	0.0
7	3.199090946316694E-5	0.0	0.0	9.597272838950084E-5	0.0
8	6.398181892633389E-5	0.0	0.0	9.597272838950084E-5	0.0
9	6.398181892633389E-5	0.0	0.0	3.5190000409483644E-4	0.0
10	6.398181892633389E-5	0.0	0.0	4.478727324843373E-4	0.0
11	6.398181892633389E-5	0.0	0.0	6.39818189263339E-4	0.0
12	6.398181892633389E-5	0.0	0.0	8.637545555055076E-4	0.0
13	9.597272838950084E-5	0.0	0.0	8.637545555055076E-4	0.0
14	9.597272838950084E-5	0.0	0.0	0.0010237091028213422	0.0
15	9.597272838950084E-5	0.0	0.0	0.0011836636501371771	0.0
16	1.5995454731583474E-4	0.0	0.0	0.0016635272920846812	0.0
17	1.5995454731583474E-4	0.0	0.0	0.002431309119200688	0.0
18	1.5995454731583474E-4	0.0	0.0	0.0028152000327586915	0.0
19	1.5995454731583474E-4	0.0	0.0	0.0037109454977273656	0.0
20	1.5995454731583474E-4	0.0	0.0	0.00575836370337005	6.398181892633389E-5
21	1.5995454731583474E-4	0.0	0.0	0.009085418287539412	6.398181892633389E-5
22	1.5995454731583474E-4	0.0	0.0	0.014747809262519963	6.398181892633389E-5
23	1.5995454731583474E-4	0.0	0.0	0.018522736579173662	6.398181892633389E-5
24	1.5995454731583474E-4	0.0	0.0	0.02472897301502805	6.398181892633389E-5
25	1.5995454731583474E-4	0.0	0.0	0.030327382171082266	6.398181892633389E-5
26	1.5995454731583474E-4	0.0	0.0	0.040916373203390526	6.398181892633389E-5
27	1.5995454731583474E-4	0.0	0.0	0.09942774661152287	6.398181892633389E-5
28	1.5995454731583474E-4	0.0	0.0	0.21942564800786207	6.398181892633389E-5
29	1.5995454731583474E-4	0.0	0.0	0.36968694975635724	6.398181892633389E-5
30	1.5995454731583474E-4	0.0	0.0	0.5933673887228206	6.398181892633389E-5
31	1.5995454731583474E-4	0.0	0.0	0.8851564739363662	6.398181892633389E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10380	0.0	24.2211	1
GTATTGG	725	0.0	16.586208	1
GTATCAA	15820	0.0	15.892225	2
TTGGACC	1325	0.0	14.939623	4
TGGACCC	1365	0.0	14.501831	5
TATCGGC	115	2.2121392E-5	14.47826	2
GGACCCT	1320	0.0	14.435607	6
CGCGATA	145	8.923307E-7	14.034483	14
ATTGGAC	910	0.0	14.0274725	3
ACGTTTA	215	1.9826984E-10	13.767442	26
TAGGACC	405	0.0	13.703704	4
GTATTAG	460	0.0	13.673912	1
CTAATAC	585	0.0	13.598291	3
TTAACGG	290	0.0	13.396551	35
TATACCG	180	5.1679308E-8	13.36111	5
GACCCTC	1400	0.0	13.346428	7
CGACGAA	265	1.8189894E-12	13.264152	10
TATACAC	1580	0.0	12.996835	37
TACACCG	215	2.904926E-9	12.906977	5
TCTATAC	275	3.6379788E-12	12.781817	3
>>END_MODULE