FastQCFastQC Report
Wed 25 May 2016
SRR2088702_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088702_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4673690
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT439650.9406914022966864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT409840.8769088236489798No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT320940.6866950953101297No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169630.3629466224760307No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG106170.22716525914213392No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG101650.2174940999510023No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA92650.19823736704830658No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA90400.19342318382263265No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG79700.1705290680383166No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA75290.16109326891599574No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA54170.1159041357043364No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA50500.10805166795401493No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC50470.10798747884433926No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT47440.10150437876709838No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA47100.10077690219077431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121150.024.5241431
GTATTGG13550.018.7047981
ATTGGAC14050.017.5124553
TTGGACC20800.016.543274
GGACCCT20000.016.0956
GTATCAA189050.015.70616152
ACGTTTA2850.014.92982626
ACCGTCG2151.2732926E-1114.62790723
GACCCTC21200.014.4858497
TGGACCC21800.014.4266055
GTATTAG8800.014.0852271
TCACGTT3100.013.12903224
TATTGGA18850.012.8567642
CCGGTCT7350.012.5850339
TACTCCG7400.012.5000015
TATACTG6550.012.4274815
TCCGGTC7750.012.4129038
CCCTCGC22600.012.360629
TAGTACT6500.012.23846154
TCACGTA7450.012.16778525
Produced by FastQC (version 0.11.5)