##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088702_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4673690
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.566812518588097	31.0	31.0	34.0	30.0	34.0
2	31.776615051490364	31.0	31.0	34.0	30.0	34.0
3	31.857638183105855	31.0	31.0	34.0	30.0	34.0
4	35.576873733602355	37.0	35.0	37.0	33.0	37.0
5	35.44472205045692	37.0	35.0	37.0	33.0	37.0
6	35.48362856757723	37.0	35.0	37.0	33.0	37.0
7	35.34866925277458	37.0	35.0	37.0	33.0	37.0
8	35.372287207752336	37.0	35.0	37.0	33.0	37.0
9	36.9728875898915	39.0	37.0	39.0	33.0	39.0
10	36.78068057573352	39.0	37.0	39.0	32.0	39.0
11	36.872171881318614	39.0	37.0	39.0	33.0	39.0
12	36.727618006329045	39.0	35.0	39.0	32.0	39.0
13	36.76832395815726	39.0	37.0	39.0	32.0	39.0
14	37.7656042655803	40.0	37.0	41.0	32.0	41.0
15	37.77846006046614	40.0	37.0	41.0	32.0	41.0
16	37.66729671843875	39.0	36.0	41.0	32.0	41.0
17	37.67618990562061	39.0	37.0	41.0	32.0	41.0
18	37.63050694419185	39.0	36.0	41.0	32.0	41.0
19	37.67243334495869	39.0	37.0	41.0	32.0	41.0
20	37.54295043102987	39.0	36.0	41.0	32.0	41.0
21	37.48048458498531	39.0	36.0	41.0	32.0	41.0
22	37.34457184794028	39.0	36.0	41.0	31.0	41.0
23	37.16846774176293	39.0	36.0	41.0	31.0	41.0
24	37.00697115127448	39.0	36.0	41.0	31.0	41.0
25	37.1999007208437	39.0	36.0	41.0	31.0	41.0
26	37.08545645945709	39.0	36.0	41.0	31.0	41.0
27	36.97524418607139	39.0	36.0	41.0	31.0	41.0
28	36.81007961589237	39.0	35.0	41.0	30.0	41.0
29	36.7065511405335	39.0	35.0	41.0	30.0	41.0
30	36.525639270041445	39.0	35.0	40.0	30.0	41.0
31	36.395042461096054	39.0	35.0	40.0	30.0	41.0
32	36.227236509053874	38.0	35.0	40.0	30.0	41.0
33	36.10179258786954	38.0	35.0	40.0	29.0	41.0
34	35.925993593926854	38.0	35.0	40.0	29.0	41.0
35	35.80332734948189	38.0	35.0	40.0	28.0	41.0
36	35.62252994956876	38.0	34.0	40.0	27.0	41.0
37	35.570419946551866	38.0	34.0	40.0	26.0	41.0
38	35.39646168231098	38.0	34.0	40.0	26.0	41.0
39	35.31853524730994	38.0	34.0	40.0	25.0	41.0
40	35.17219156597891	38.0	34.0	40.0	25.0	41.0
41	35.060985003284344	38.0	34.0	40.0	24.0	41.0
42	34.82747400875967	38.0	33.0	40.0	23.0	41.0
43	34.49444379066647	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	2.0
14	12.0
15	25.0
16	49.0
17	130.0
18	319.0
19	788.0
20	1706.0
21	3470.0
22	6538.0
23	11294.0
24	18207.0
25	28072.0
26	40959.0
27	57419.0
28	77006.0
29	100293.0
30	128521.0
31	159209.0
32	194525.0
33	238317.0
34	293265.0
35	360172.0
36	460634.0
37	627825.0
38	863490.0
39	1001440.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.31183283444131	21.12356617576262	13.545571058414229	24.01902993138184
2	19.00314312673712	23.10007724089531	34.690084280301	23.20669535206657
3	19.5855737115641	24.364131981368043	32.28857712000582	23.761717187062043
4	13.915385915625556	16.473471710789546	36.20629523994959	33.40484713363531
5	12.880229540256199	38.30669128675629	35.126655811575006	13.686423361412503
6	32.67953159067032	37.34779157368161	14.000564864165147	15.972111971482919
7	26.452567457405173	32.71543469934891	21.702209603118735	19.12978824012718
8	26.82835190181634	34.66586358958339	19.21201448962169	19.293770018978577
9	24.89559641311255	14.365565538150799	21.439248217147476	39.299589831589174
10	16.142940588699723	27.84848802552159	34.08538007441658	21.923191311362114
11	33.5221848261224	22.490537455415314	23.26975045413795	20.717527264324335
12	20.791152173122306	26.997768358620277	31.085373655505606	21.125705812751807
13	31.10867430231787	21.125427659943213	25.985762855473943	21.780135182264974
14	20.669749170355757	22.740404263012735	27.51384880041252	29.07599776621898
15	22.98263256655876	29.644456521506562	24.926492771236433	22.44641814069825
16	22.255027612015347	28.33690296104363	26.313255693039117	23.094813733901905
17	21.763766959297687	27.216610429874464	26.993724444710708	24.025898166117138
18	22.866129332497447	26.117650079487515	28.986368372741882	22.029852215273156
19	22.816489754348275	25.385915625554965	29.97832547729952	21.819269142797232
20	23.65790200034662	25.143901285707866	29.598967839116412	21.599228874829095
21	22.606484383859435	25.843091861034857	30.074202610785054	21.476221144320654
22	21.6727681981475	26.090048762326983	29.76470412029895	22.472478919226564
23	22.444449674668196	26.264386384206055	30.292552565531732	20.998611375594017
24	22.382100652803246	26.21887630544602	28.559981513536414	22.839041528214324
25	22.441603957472577	26.33529395402776	29.45250968720647	21.770592401293197
26	22.06430037079909	26.983111845244334	29.33587807492581	21.616709709030765
27	21.216597592052533	27.033307729010698	29.154265687283498	22.595828991653278
28	21.773331136639356	26.62502219873376	29.236021216640385	22.365625447986496
29	21.85957990367354	26.60850420117723	28.820139975051834	22.711775920097395
30	22.0497722356425	26.828437487295904	29.89089990992128	21.23089036714031
31	22.574774963679662	26.608333030218095	29.634635587726187	21.18225641837606
32	22.149843057626843	26.490481824853596	29.098891882003297	22.260783235516264
33	21.821815310814365	26.434936848614264	29.559256176597078	22.18399166397429
34	21.281321610975482	26.688269868134174	30.120119220573038	21.91028930031731
35	21.0136744199979	28.141917842218888	29.28354255417026	21.560865183612947
36	22.616925812366674	26.486994216561214	29.080555193005953	21.815524778066152
37	21.891289323853318	26.750661682738908	29.27720922868226	22.080839764725514
38	21.696218619549008	25.839754027331725	30.25868211199288	22.20534524112639
39	21.208552556973185	25.6874332700714	30.90780518177286	22.196208991182555
40	20.48090480968999	25.94819082994379	30.748552000667566	22.82235235969865
41	21.448277485241853	25.149999251127053	30.75007114292989	22.6516521207012
42	20.29434986060265	26.73217521915232	30.318527758580476	22.654947161664552
43	20.647004829160682	26.447111383082746	29.821768238800605	23.084115548955964
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1743.0
1	1523.0
2	1303.0
3	2370.0
4	3437.0
5	3437.0
6	5076.0
7	6715.0
8	7373.0
9	8031.0
10	12162.5
11	16294.0
12	16294.0
13	30407.5
14	44521.0
15	68668.5
16	92816.0
17	87409.5
18	82003.0
19	82003.0
20	89967.0
21	97931.0
22	82945.5
23	67960.0
24	77615.0
25	87270.0
26	87270.0
27	97723.0
28	108176.0
29	116914.0
30	125652.0
31	138627.5
32	151603.0
33	151603.0
34	168739.0
35	185875.0
36	201928.5
37	217982.0
38	230365.0
39	242748.0
40	242748.0
41	251467.0
42	260186.0
43	271692.0
44	283198.0
45	297437.5
46	311677.0
47	311677.0
48	379619.5
49	447562.0
50	421625.0
51	395688.0
52	363754.0
53	331820.0
54	331820.0
55	302711.0
56	273602.0
57	240296.0
58	206990.0
59	188841.5
60	170693.0
61	170693.0
62	151980.5
63	133268.0
64	117075.0
65	100882.0
66	87105.5
67	73329.0
68	73329.0
69	62970.5
70	52612.0
71	44555.5
72	36499.0
73	29955.5
74	23412.0
75	23412.0
76	18682.0
77	13952.0
78	10791.5
79	7631.0
80	5973.5
81	4316.0
82	4316.0
83	3253.5
84	2191.0
85	1633.0
86	1075.0
87	817.5
88	560.0
89	560.0
90	420.0
91	280.0
92	202.5
93	125.0
94	90.5
95	56.0
96	56.0
97	35.5
98	15.0
99	13.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4673690.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.42268404655493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.7929327216027	35.943043014183594
2	13.141006949904863	12.463636412778415
3	4.63651585267132	6.596280790742257
4	2.139526752885487	4.058484048449602
5	1.1435489624652866	2.7115080569378494
6	0.6710528427913093	1.9093876165340855
7	0.443248408419098	1.4714020458618053
8	0.2977532234912779	1.1296205633176104
9	0.23489709527360053	1.0025505659352123
>10	1.27437613973765	11.402890753073153
>50	0.1306157543505758	4.342910362794986
>100	0.08476864150939876	7.524549530946787
>500	0.005736551995897724	1.8454776052954578
>1k	0.0034328968594948906	3.2261689146770904
>5k	3.1618786863767915E-4	1.0564251944362435
>10k+	2.710181731180107E-4	3.3156645240357934
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	43965	0.9406914022966864	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	40984	0.8769088236489798	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	32094	0.6866950953101297	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16963	0.3629466224760307	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	10617	0.22716525914213392	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	10165	0.2174940999510023	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	9265	0.19823736704830658	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9040	0.19342318382263265	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7970	0.1705290680383166	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7529	0.16109326891599574	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	5417	0.1159041357043364	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5050	0.10805166795401493	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	5047	0.10798747884433926	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4744	0.10150437876709838	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4710	0.10077690219077431	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.1396369891884144E-5	0.0
3	0.0	0.0	0.0	2.1396369891884144E-5	0.0
4	0.0	0.0	0.0	2.1396369891884144E-5	0.0
5	0.0	2.1396369891884144E-5	0.0	2.1396369891884144E-5	0.0
6	4.279273978376829E-5	2.1396369891884144E-5	0.0	2.1396369891884144E-5	0.0
7	4.279273978376829E-5	2.1396369891884144E-5	0.0	8.558547956753658E-5	0.0
8	6.418910967565244E-5	2.1396369891884144E-5	0.0	8.558547956753658E-5	0.0
9	1.0698184945942072E-4	2.1396369891884144E-5	0.0	1.7117095913507315E-4	0.0
10	1.2837821935130487E-4	2.1396369891884144E-5	0.0	1.7117095913507315E-4	0.0
11	1.49774589243189E-4	4.279273978376829E-5	0.0	2.5675643870260974E-4	0.0
12	1.49774589243189E-4	4.279273978376829E-5	0.0	2.99549178486378E-4	0.0
13	1.49774589243189E-4	4.279273978376829E-5	0.0	3.209455483782621E-4	0.0
14	2.1396369891884143E-4	4.279273978376829E-5	0.0	3.8513465805391456E-4	0.0
15	2.1396369891884143E-4	4.279273978376829E-5	0.0	5.777019870808718E-4	0.0
16	2.1396369891884143E-4	4.279273978376829E-5	0.0	8.98647535459134E-4	0.0
17	2.3536006881072556E-4	4.279273978376829E-5	0.0	0.0013051785634049327	0.0
18	2.3536006881072556E-4	4.279273978376829E-5	0.0	0.0018186914408101522	0.0
19	2.3536006881072556E-4	4.279273978376829E-5	0.0	0.0023322043182153716	0.0
20	2.3536006881072556E-4	4.279273978376829E-5	0.0	0.004129499389133639	0.0
21	2.5675643870260974E-4	4.279273978376829E-5	0.0	0.0069752165847542305	0.0
22	2.5675643870260974E-4	4.279273978376829E-5	0.0	0.010527013986806999	0.0
23	2.5675643870260974E-4	6.418910967565244E-5	0.0	0.013265749332968169	0.0
24	2.5675643870260974E-4	6.418910967565244E-5	0.0	0.01793015796939891	0.0
25	2.5675643870260974E-4	6.418910967565244E-5	0.0	0.021524748111235448	0.0
26	2.7815280859449387E-4	6.418910967565244E-5	0.0	0.029420008601340697	0.0
27	2.7815280859449387E-4	6.418910967565244E-5	0.0	0.0763636441441345	0.0
28	2.7815280859449387E-4	6.418910967565244E-5	0.0	0.1714919046834514	0.0
29	2.7815280859449387E-4	8.558547956753658E-5	0.0	0.29079806320059737	0.0
30	2.7815280859449387E-4	8.558547956753658E-5	0.0	0.461883436856103	0.0
31	2.7815280859449387E-4	8.558547956753658E-5	0.0	0.6806613190006183	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12115	0.0	24.524143	1
GTATTGG	1355	0.0	18.704798	1
ATTGGAC	1405	0.0	17.512455	3
TTGGACC	2080	0.0	16.54327	4
GGACCCT	2000	0.0	16.095	6
GTATCAA	18905	0.0	15.7061615	2
ACGTTTA	285	0.0	14.929826	26
ACCGTCG	215	1.2732926E-11	14.627907	23
GACCCTC	2120	0.0	14.485849	7
TGGACCC	2180	0.0	14.426605	5
GTATTAG	880	0.0	14.085227	1
TCACGTT	310	0.0	13.129032	24
TATTGGA	1885	0.0	12.856764	2
CCGGTCT	735	0.0	12.585033	9
TACTCCG	740	0.0	12.500001	5
TATACTG	655	0.0	12.427481	5
TCCGGTC	775	0.0	12.412903	8
CCCTCGC	2260	0.0	12.36062	9
TAGTACT	650	0.0	12.2384615	4
TCACGTA	745	0.0	12.167785	25
>>END_MODULE