FastQCFastQC Report
Wed 25 May 2016
SRR2088701_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088701_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2568361
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT305431.1892019852349418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT274591.0691254072149514No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT217560.8470771826857673No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116900.45515408464775786No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG67900.26437093539420664No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG67100.2612561084676181No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA61680.24015315603998036No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA56900.22154206515361355No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG52990.2063183485499118No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA47700.18572155549784472No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA37300.14522880545219305No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC34840.13565071265293313No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT33630.13093953692646787No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA31240.12163399148328448No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA29550.11505391960086608No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29520.114937113591119No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27700.10785088233312995No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA27280.10621559819667095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86400.023.2748851
GTATTGG8350.020.8263471
ATTGGAC9150.019.0054633
TTGGACC12600.017.7658734
TGCGACG1054.802969E-717.61904722
GGACCCT12500.016.286
TACCCCG2500.016.2799995
CGAATTA1151.2435794E-616.08695615
TGGACCC13250.016.0566045
TATACTG3750.015.7866685
GTATACT2350.015.7446814
GACCCTC13200.015.6969697
TAATACT6650.015.5789474
TCTATAC2300.015.2826093
CTAATAC4800.015.0312493
GTATTAG4850.014.8762881
GTATCAA135400.014.8382562
TGTACCG750.00410624214.7999995
TATTGGA11750.014.327662
CCCTCGC13500.013.9777789
Produced by FastQC (version 0.11.5)