##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088701_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2568361
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.563390816166418	31.0	31.0	34.0	30.0	34.0
2	31.77392002136771	31.0	31.0	34.0	30.0	34.0
3	31.849127128156827	31.0	31.0	34.0	30.0	34.0
4	35.56259614594677	37.0	35.0	37.0	33.0	37.0
5	35.44126234590854	37.0	35.0	37.0	33.0	37.0
6	35.494283708559664	37.0	35.0	37.0	33.0	37.0
7	35.347111251105275	37.0	35.0	37.0	33.0	37.0
8	35.385996361103444	37.0	35.0	37.0	33.0	37.0
9	36.97525581489518	39.0	37.0	39.0	33.0	39.0
10	36.79457560677802	39.0	37.0	39.0	32.0	39.0
11	36.88461785551174	39.0	37.0	39.0	33.0	39.0
12	36.73253331599413	39.0	35.0	39.0	32.0	39.0
13	36.7704318045633	39.0	37.0	39.0	32.0	39.0
14	37.748058392102976	40.0	37.0	41.0	32.0	41.0
15	37.76426911948904	40.0	37.0	41.0	32.0	41.0
16	37.65770894356362	39.0	36.0	41.0	32.0	41.0
17	37.6898450023186	39.0	37.0	41.0	32.0	41.0
18	37.6556539364988	39.0	37.0	41.0	32.0	41.0
19	37.70483588560954	40.0	37.0	41.0	32.0	41.0
20	37.586228337838804	39.0	37.0	41.0	32.0	41.0
21	37.52499551270246	39.0	36.0	41.0	32.0	41.0
22	37.39859427860803	39.0	36.0	41.0	32.0	41.0
23	37.208625656595785	39.0	36.0	41.0	31.0	41.0
24	37.05176375127951	39.0	36.0	41.0	31.0	41.0
25	37.2269821103809	39.0	36.0	41.0	31.0	41.0
26	37.1009383026763	39.0	36.0	41.0	31.0	41.0
27	36.98625543683306	39.0	36.0	41.0	31.0	41.0
28	36.812020584333744	39.0	35.0	41.0	30.0	41.0
29	36.698472683551884	39.0	35.0	41.0	30.0	41.0
30	36.489753192794936	39.0	35.0	40.0	30.0	41.0
31	36.334544481869955	38.0	35.0	40.0	30.0	41.0
32	36.158149107543686	38.0	35.0	40.0	30.0	41.0
33	36.01596348799877	38.0	35.0	40.0	29.0	41.0
34	35.82986581714954	38.0	35.0	40.0	28.0	41.0
35	35.698441924635986	38.0	35.0	40.0	27.0	41.0
36	35.49883641746624	38.0	34.0	40.0	27.0	41.0
37	35.4203906693802	38.0	34.0	40.0	26.0	41.0
38	35.232929483043854	38.0	34.0	40.0	25.0	41.0
39	35.131946404730485	38.0	34.0	40.0	25.0	41.0
40	34.963555356898816	38.0	34.0	40.0	24.0	41.0
41	34.82864714111451	38.0	33.0	40.0	23.0	41.0
42	34.564000932890664	38.0	33.0	40.0	23.0	41.0
43	34.20616377526368	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	2.0
13	1.0
14	5.0
15	11.0
16	30.0
17	70.0
18	176.0
19	414.0
20	966.0
21	1899.0
22	3706.0
23	6383.0
24	10248.0
25	15613.0
26	23346.0
27	32108.0
28	43138.0
29	56600.0
30	71305.0
31	87985.0
32	107754.0
33	132602.0
34	163125.0
35	198458.0
36	254884.0
37	351941.0
38	468781.0
39	536808.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.07604032299198	21.436900809504582	14.147660706575127	23.33939816092831
2	18.92619456532785	23.52667712988945	34.884192681636264	22.662935623146435
3	19.7095346020283	24.45388323526171	32.81232661607928	23.024255546630712
4	13.815736962210531	16.57570723118752	36.0842186904411	33.52433711616085
5	12.615010117347211	38.64507364813591	34.981024863716584	13.758891370800288
6	32.38536171511715	37.33844268776858	13.695582513517376	16.580613083596894
7	25.7987876314895	33.31474819933803	21.565582096909274	19.320882072263206
8	26.845213737476936	34.69960025089931	19.13395352133131	19.32123249029245
9	24.525290642553756	14.567656182289015	21.92573396029608	38.98131921486115
10	16.273919437337664	28.055713351822426	33.61081249871027	22.059554712129643
11	32.92695224697774	22.684934088315465	23.506781172895867	20.881332491810927
12	20.76433180538094	27.537873375277073	31.057666737658764	20.64012808168322
13	31.246464184746614	21.324805975483976	26.297821840465573	21.130907999303837
14	20.258328171156627	23.305680159447988	28.054856774417615	28.381134894977773
15	22.4846896522724	29.916004798390883	25.62073633729838	21.978569212038337
16	21.539339680052766	28.64110613733817	26.889249603151583	22.930304579457484
17	21.385895518581695	27.342534791643384	27.32174332190841	23.94982636786651
18	22.66281881713669	26.0667016825127	29.70641588156805	21.564063618782562
19	22.449569978675115	25.34624221439276	30.890867755739944	21.313320051192182
20	23.279827095957305	25.02370188614451	30.67571108578584	21.020759932112348
21	22.140812759577024	25.86271945415773	31.205192727969315	20.791275058295934
22	21.04030547107669	26.10653253183645	30.717722313958202	22.13543968312866
23	21.96864070120984	26.341117934745157	31.365450573342297	20.32479079070271
24	21.886798623713723	26.376276543679023	29.358684390551016	22.37824044205624
25	21.764385925498793	26.63274360574701	30.355117524366708	21.24775294438749
26	21.464194480448814	27.277045555511865	30.217987268923647	21.040772695115677
27	20.597104534759715	27.248389147787243	30.043595896371265	22.110910421081773
28	21.086327038917037	26.879360027659665	30.11270611880495	21.92160681461835
29	21.24016055375393	26.714040588530974	29.687571178662193	22.358227679052906
30	21.430515414305077	27.033816507881873	30.966207632026805	20.56946044578624
31	22.01438972169411	26.821385311488534	30.727183600747715	20.437041366069643
32	21.525634441575775	26.771119791960707	29.88777667936867	21.815469087094844
33	21.190868417640665	26.65949218197909	30.465031979538704	21.68460742084154
34	20.479130464915173	26.936205619069902	31.132344713223723	21.452319202791195
35	20.196654598010173	28.58671347213262	30.077158156505256	21.139473773351956
36	22.040787879896946	26.61794817784572	29.98020916841519	21.36105477384215
37	21.147182969995264	27.180641662133944	30.054030566575335	21.618144801295454
38	21.026016202550966	25.98365260958253	31.1953031524774	21.795028035389105
39	20.590797010233373	25.714998787164266	31.909494031407576	21.78471017119478
40	19.64696551614045	26.10816781597291	31.674752887152547	22.570113780734093
41	20.791898023681252	25.316145199214596	31.68709538884915	22.204861388255
42	19.692013700566235	27.037164946827957	30.993618108980787	22.27720324362502
43	19.96588485808654	26.8799440577084	30.403630953748323	22.750540130456738
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	910.0
1	870.0
2	830.0
3	1420.0
4	2010.0
5	2010.0
6	3229.0
7	4448.0
8	4840.5
9	5233.0
10	8108.5
11	10984.0
12	10984.0
13	20610.5
14	30237.0
15	46913.5
16	63590.0
17	58851.0
18	54112.0
19	54112.0
20	58400.5
21	62689.0
22	51519.5
23	40350.0
24	45164.5
25	49979.0
26	49979.0
27	55584.0
28	61189.0
29	65807.5
30	70426.0
31	76599.0
32	82772.0
33	82772.0
34	92041.5
35	101311.0
36	109766.0
37	118221.0
38	124351.5
39	130482.0
40	130482.0
41	134758.0
42	139034.0
43	145863.5
44	152693.0
45	161012.5
46	169332.0
47	169332.0
48	215032.5
49	260733.0
50	241803.5
51	222874.0
52	202235.5
53	181597.0
54	181597.0
55	163298.5
56	145000.0
57	125391.5
58	105783.0
59	95507.5
60	85232.0
61	85232.0
62	75187.5
63	65143.0
64	56892.5
65	48642.0
66	41817.5
67	34993.0
68	34993.0
69	29810.5
70	24628.0
71	20893.5
72	17159.0
73	14118.0
74	11077.0
75	11077.0
76	8935.0
77	6793.0
78	5232.0
79	3671.0
80	2861.5
81	2052.0
82	2052.0
83	1568.0
84	1084.0
85	819.5
86	555.0
87	412.5
88	270.0
89	270.0
90	209.5
91	149.0
92	106.5
93	64.0
94	41.5
95	19.0
96	19.0
97	13.5
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2568361.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.18672985634532
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.02975122225325	40.452745264276544
2	11.897814553096934	12.180204388205334
3	3.943456184534565	6.055578792543151
4	1.660802897959175	3.400442771298869
5	0.8856529492760947	2.2666839130535483
6	0.5196819339247244	1.5960491257816782
7	0.34289078330413186	1.228602052665347
8	0.24779454658547345	1.0147034012756966
9	0.1841524598719268	0.8483545994261212
>10	1.103420194782241	10.722971708986465
>50	0.10787236191278059	3.8340008536149552
>100	0.06788134254448165	6.5314467387196835
>500	0.004262068253809484	1.5258437607701383
>1k	0.0038815264454327226	3.5854815626985963
>5k	3.805418083757571E-4	1.1943164443006091
>10k+	3.0443344670060565E-4	3.5625746223832113
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	30543	1.1892019852349418	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	27459	1.0691254072149514	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	21756	0.8470771826857673	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11690	0.45515408464775786	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6790	0.26437093539420664	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6710	0.2612561084676181	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6168	0.24015315603998036	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5690	0.22154206515361355	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5299	0.2063183485499118	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4770	0.18572155549784472	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3730	0.14522880545219305	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3484	0.13565071265293313	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3363	0.13093953692646787	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3124	0.12163399148328448	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2955	0.11505391960086608	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2952	0.114937113591119	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2770	0.10785088233312995	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2728	0.10621559819667095	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	3.893533658235739E-5	0.0	0.0	0.0	0.0
7	3.893533658235739E-5	0.0	0.0	0.0	0.0
8	3.893533658235739E-5	0.0	0.0	0.0	0.0
9	7.787067316471478E-5	0.0	0.0	3.893533658235739E-5	0.0
10	7.787067316471478E-5	0.0	0.0	7.787067316471478E-5	0.0
11	7.787067316471478E-5	0.0	0.0	1.9467668291178693E-4	0.0
12	7.787067316471478E-5	0.0	0.0	4.2828870240593125E-4	0.0
13	7.787067316471478E-5	0.0	0.0	5.450947121530034E-4	0.0
14	7.787067316471478E-5	0.0	0.0	7.787067316471477E-4	0.0
15	7.787067316471478E-5	0.0	0.0	9.733834145589347E-4	0.0
16	1.1680600974707216E-4	0.0	0.0	0.0014406074535472232	0.0
17	1.1680600974707216E-4	0.0	0.0	0.0025307968778532303	0.0
18	1.1680600974707216E-4	0.0	0.0	0.003153762263170948	0.0
19	1.5574134632942956E-4	0.0	0.0	0.004127145677729883	0.0
20	1.9467668291178693E-4	0.0	0.0	0.006463265872671326	0.0
21	1.9467668291178693E-4	0.0	0.0	0.010551476213818852	0.0
22	1.9467668291178693E-4	0.0	0.0	0.016742194730413676	0.0
23	1.9467668291178693E-4	0.0	0.0	0.020246375022825842	0.0
24	1.9467668291178693E-4	0.0	0.0	0.02709899426132074	0.0
25	1.9467668291178693E-4	0.0	0.0	0.032744618065762565	0.0
26	1.9467668291178693E-4	0.0	0.0	0.04306248226008727	0.0
27	1.9467668291178693E-4	0.0	0.0	0.10356799530907065	0.0
28	1.9467668291178693E-4	0.0	0.0	0.2237613793388079	0.0
29	1.9467668291178693E-4	0.0	0.0	0.37420751989303686	0.0
30	1.9467668291178693E-4	0.0	0.0	0.592868370139556	0.0
31	1.9467668291178693E-4	0.0	0.0	0.8631964120308633	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8640	0.0	23.274885	1
GTATTGG	835	0.0	20.826347	1
ATTGGAC	915	0.0	19.005463	3
TTGGACC	1260	0.0	17.765873	4
TGCGACG	105	4.802969E-7	17.619047	22
GGACCCT	1250	0.0	16.28	6
TACCCCG	250	0.0	16.279999	5
CGAATTA	115	1.2435794E-6	16.086956	15
TGGACCC	1325	0.0	16.056604	5
TATACTG	375	0.0	15.786668	5
GTATACT	235	0.0	15.744681	4
GACCCTC	1320	0.0	15.696969	7
TAATACT	665	0.0	15.578947	4
TCTATAC	230	0.0	15.282609	3
CTAATAC	480	0.0	15.031249	3
GTATTAG	485	0.0	14.876288	1
GTATCAA	13540	0.0	14.838256	2
TGTACCG	75	0.004106242	14.799999	5
TATTGGA	1175	0.0	14.32766	2
CCCTCGC	1350	0.0	13.977778	9
>>END_MODULE